Testing implications of varying targets for Bordetella pertussis: Comparison of the FilmArray Respiratory Panel and the Focus B. pertussis PCR assay

Journal of Clinical Pathology

Volumn 68, Issue 5, 2015, Pages 394-396

  Jerris, Robert C ^a,b   Williams, Sally R ^a   MacDonald, Heather J ^a   Ingebrigtsen, Danielle R ^a   Westblade, Lars F ^a,b   Rogers, Beverly B ^a,b  

^a CHILDREN'S HEALTHCARE OF ATLANTA   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PERTUSSIS TOXIN; BACTERIAL DNA; GENETIC MARKER;

ADOLESCENT; ADULT; ARTICLE; BACTERIAL GENE; BORDETELLA PERTUSSIS; CHILD; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; GENE INSERTION SEQUENCE; HUMAN; INFANT; INTERMETHOD COMPARISON; MULTIPLEX POLYMERASE CHAIN REACTION; PERTUSSIS; POLYMERASE CHAIN REACTION SYSTEM; PRIORITY JOURNAL; PROMOTER REGION; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; TISSUE SECTION; COMPARATIVE STUDY; EVALUATION STUDY; GENETIC MARKER; GENETICS; MICROBIOLOGY; PATHOGENICITY; PREDICTIVE VALUE; PRESCHOOL CHILD; REPRODUCIBILITY; WHOOPING COUGH;

ADOLESCENT; BORDETELLA PERTUSSIS; CHILD; CHILD, PRESCHOOL; DNA, BACTERIAL; GENETIC MARKERS; HUMANS; INFANT; MULTIPLEX POLYMERASE CHAIN REACTION; PERTUSSIS TOXIN; PREDICTIVE VALUE OF TESTS; PROMOTER REGIONS, GENETIC; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; WHOOPING COUGH;

EID: 84928158828     PISSN: 00219746     EISSN: 14724146     Source Type: Journal    
DOI: 10.1136/jclinpath-2014-202833     Document Type: Article

References (12)

1. Rodgers L, Martin SW, Cohn A, et al. Epidemiologic and laboratory features of a large outbreak of pertussis-like illnesses associated with cocirculating Bordetella holmesii and Bordetella pertussis-Ohio, 2010-2011. Clin Infect Dis 2013;56:322-31.
2. Tatti KM, Sparks KN, Boney KO, et al. Novel multitarget real-time PCR assay for rapid detection of Bordetella species in clinical specimens. J Clin Microbiol 2011;49:4059-66.
3. Tizolova A, Guiso N, Guillot S. Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome. Eur J Clin Microbiol Infect Dis 2013;32:89-96.
4. Bottero D, Griffith MM, Lara C, et al. Bordetella holmesii in children suspected of pertussis in Argentina. Epidemiol Infection 2013;141:714-17.
5. Reischl U, Lehn N, Sanden GN, et al. Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii. J Clin Microbiol, 2001;39:1963-6.
6. Loeffelholz MJ, Thompson CJ, Long KS, et al. Detection of Bordetella holmesii using Bordetella pertussis IS481 PCR assay. J Clin Microbiol 2000;38:467.
7. Xu Y, Xu Y, Hou Q, et al. Triplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis. APMIS, 2010;118:685-91.
8. Poritz MA, Blaschke AJ, Byington CL, et al. FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection. PLoS ONE 2011;6:e26047.
9. Couturier MR, Barney T, Alger G, et al. Evaluation of the FilmArray (R) Respiratory Panel for clinical use in a large children's hospital. J Clin Lab Anal 2013;27:148-54.
10. Pierce VM, Elkan M, Leet M, et al. Comparison of the Idaho Technology FilmArray system to real-time PCR for detection of respiratory pathogens in children. J Clin Microbiol 2012;50:364-71.
11. Glare EM, Paton JC, Premier RR, et al. Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction. J Clin Microbiol 1990;28:1982-7.
12. Qin X, Galanakis E, Martin ET, et al. Multitarget PCR for Diagnosis of Pertussis and Its Clinical Implications. J Clin Microbiol 2007;45:506-11. doi:10.1128/JCM. 02042-06