Minimal biofilm eradication concentration (MBEC) assay: Susceptibility testing for biofilms

Biofilms, Infection, and Antimicrobial Therapy

Volumn , Issue , 2005, Pages 257-269

  Ceri, Howard ^a   Olson, Merle E ^a   Morck, Douglas W ^a   Storey, Douglas G ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 85057693750     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (65)

1. Parsek, M.R., and Sing, P.K., Bacterial biofilms: an emerging link to disease pathogens. Annu. Rev. Microbiol. 57, 677-701, 2003.
2. Olson, M.E., Ceri, H., and Morck, D.W., Interaction of biofilms with tissues. In: Jass, J., Surman, S., and Walker, J., eds., Medical Biofilms Detection, Prevention and Control. Chichester: Wiley, 2003:125-148.
3. Donlan, R.M., and Costerton, J.W., Biofilms: survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev. 15, 167-193, 2002.
4. Costerton, J.W., Stewart, P.S., and Greenberg, E.P., Bacterial biofilms: a common cause of persistent infections. Science 284, 1318-1322, 1999.
5. Jass, J., Shurman, S., and Walker, J.T., Microbial biofilms in medicine. In: Jass, J., Surman, S., Walker, J., eds., Medical Biofilms Detection, Prevention and Control. Chichester: Wiley, 2003:1-28.
6. Ofek, I., and Beachey, E.H., General concepts and principals of bacterial adherence in animals and man. In: Beachey, E.H., ed., Bacteria Adherence. London: Chapman and Hall, 1980:1-29.
7. Buret, A., Ward, K.H., Olson, M.E., and Costerton, J.W., An in vitro model to study the pathobiology of infectious biofilms on biomaterial surfaces. J. Biomed. Mater. Res. 25, 865-874, 1991.
8. Veenstra, G.J., Cremers, F.F., van Dijk, H., and Fleer, A., Ultrastructural organization and regulation of a biomaterial adhesin in Staphylococal epidermidis. J. Bacteriol. 178, 537-541, 1996.
9. Herrmann, M., Vaudaux, P.E., Pittet, D., Auckenthaler, R., Lew P.D., Schumacher-Perdeau, F., Peters, G., and Waldvogel, F.A., Fibronectin, fibrinogen and laminin act as mediators of adherence of clinical staphylococcal isolates to foreign material. J. Infect. Dis. 158, 693-701, 1988.
10. Cheung, A.I., Projan, S.J., Edelstein, R.E., and Fischetti, V.A., Cloning, expression, and nucleotide sequence of a Staphylococcus aureus gene (fbpA) encoding a fibrogenbinding protein. Infect. Immun. 63, 1914-1920, 1995.
11. Donlan, R.M., Problems of biofilms associated with medical devices and implants. In: Jass, J., Surman, S., and Walker, J., eds., Medical Biofilms Detection, Prevention and Control. Chichester: Wiley, 2003:31-49.
12. Rogers, J., Norkett, D.I., Bracegirdle, P., Dowsett, A.B., Walker, J.T., Brooks, T., and Keevil, C.W., Examination of biofilm formation and risk of infection associated with the use of urinary catheters with leg bags. J. Hosp. Infect. 32, 105-115, 1996.
13. Adair, C.G., Gorman, S.P., Feron, B.M., Byers, L.M., Jones, D.S., Goldsmith, C.E., Moore, J.E., Kerr, J.R., Curran, M.D., Hogg, G., Webb, C.H., McCarthy, G.J., and Milligan, K.R., Implications of endotracheal tube biofilm for ventilator-associated pneumonia. Int. Care. Med. 25, 1072-1076, 1999.
14. Riofolo, C., Devys, C., Meunier, G., Perraud, M., and Goullet, D., Quantitative determination of endotoxins released by biofilms. J. Hosp. Infect. 43, 203-209, 1999.
15. Shiau, A-L., and Wu, C-L., The inhibitory effect of Staphylococcus epidermidis slime on the phagocytosis of murine peritoneal macrophages is interferon independent. Microbiol. Immun. 42, 33-30, 1998.
16. Yasuda, H., Akiki, Y., Aoyama, J., and Yokota, T., Interaction between human polymorphonuclear leucocyte and bacteria released from in vitro bacterial biofilm models. J. Med. Microbiol. 41, 359-367, 1994.
17. Ward, K.H., Olson, M.E., Lam, K., and Costerton, J.W., Mechanism of persistent infection associated with peritoneal implants. J. Med. Microbiol. 36, 406-413, 1992.
18. Ceri, H., Schmidt, S., Olson, M.E., Nickel, J.C., and Benediktsson, H., Specific mucosal immunity in the pathophysiology of bacterial prostatitis in a rat model. Can. J. Microbiol. 45, 849-855, 1999.
19. Graham, L.L., Ceri, H., and Costerton, J.W., Lectin-like proteins from uroepithelial cells which inhibit in vitro adherence of three urethral bacterial isolates to uroepithelial cells. Microbial Ecol. in Health and Disease 5, 77-86, 1992.
20. Lang, M.D., Nickel, J.C., Olson, M.E., Howard, S., and Ceri, H., A rat model of experimentally induced abacterial prostatitis. Prostate 45, 201-206, 2000.
21. Rippere-Lampe, K.E., Lang, M.D., Ceri, H., Olson, M.E., Lockman, H.A., and O’Brien, A.D., Cytotoxic Necrotizing Factor Type 1 causes increased inflammation and tissue damage to the prostate in a rat prostatitis model. Infect. Immun. 69, 6515-6519, 2001.
22. Lam, J., Chan, R., Lam, K., and Costerton, J.W., Production of mucoid microcolonies by Pseudomonas aeruginosa within infected lungs in cystic fibrosis. Infect. Immun. 28, 546-556, 1980.
23. Singh, P.K., Schaefer, A.L., Parsek, M.R., Moninger, T.O., Welsh, M.J., and Greenberg, E.P., Quorum-sensing signals indicate the cystic fibrosis lungs are infected with bacterial biofilms. Nature 407, 762-764, 2000.
24. Hoiby, N., Krough, J.H., Moser, C., Song, Z., Ciofu, O., and Kharazmi, A., Pseudomonas aeruginosa and the in vitro and in vitro biofilm mode of growth. Microbes. Infect. 3, 23-35, 2001.
25. Ceri, H., Olson, M.E., Stremick, C., Read, R.R., Morck, D., and Buret, A., The Calgary Biofilm Device: A new technology for the rapid determination of antibiotic susceptibility of bacterial biofilms. J. Clin. Microbiol. 37, 1771-1776, 1999.
26. Ceri, H., Olson, M.E., and Nickel, J.C., Prostatitis: role of the animal model. In: Nickel, J.C., ed., Textbook of Prostatitis. Oxford: Isis Medical Media, 1999:109-114.
27. Anderson, G.G., Palermo J.J., Schilling J.D., Roth R., and Heuser J., Hultgren S.J., Intracellular bacterial biofilm-like pods in urinary tract infections. Science 301, 105-107, 2003.
28. Justice, S.S., Hung, C., Theriot, J.A., Fletcher, D.A., Anderson, G.G., Footer M.J., and Hultgren, S.J., Differentiation and developmental pathways of uropathogenic Escherichia coli in urinary tract pathogenesis. Proc. Natl. Acad. Sci. USA 101, 1333-1338, 2004.
29. Stewart, P.S., Mechanisms of antibiotic resistance in bacterial biofilms. Int. J. Med. Microbiol. 292, 107-113, 2002.
30. Gilbert, P., Allison, D.G., and McBain, A.J., Biofilms in vitro and in vitro: do singular mechanisms imply cross-resistance? J. Appl. Microbiol. 92, Suppl, 98S-110S, 2002.
31. Lewis, K., Riddle of biofilm resistance. Antimicrob. Agents Chemother. 45, 999-1007, 2001.
32. Costerton, J.W., Cheng, K.J., Geesey, G.G., Ladd, T.I., Nickel, J.C., Dasgupta, M., and Marrie, T.J., Bacterial biofilms in nature and disease. Annu. Rev. Microbiol. 41, 435-464, 1987.
33. Stewart, P.S., Diffusion in biofilms. J. Bacteriol. 185, 1485-1491, 2003.
34. Zheng, Z., and Stewart, P.S., Penetration of rifampin through Staphylococcus epidermidis biofilms. Antimicrob. Agents Chemother. 46, 900-903, 2002.
35. Anderl, J.N., Franklin, M.J., and Stewart, P.S., Role of antibiotic penetration limitation in Klebsiella pneumoniae biofilm resistance to ampicillin and ciprofloxacin. Antimicrob. Agents Chemother. 44, 1818-1824, 2000.
36. Brown, M.R., Allison, D.G., and Gilbert, P., Resistance of bacterial biofilms to antibiotics: a growth-rate related effect? J. Antimicrob. Chemother. 22, 777-780, 1988.
37. Walters, M.C. 3rd, Roe, F., Bugnicourt, A., Franklin, M.J., and Stewart, P.S., Contributions of antibiotic penetration, oxygen limitation, and low metabolic activity to tolerance of Pseudomonas aeruginosa biofilms to ciprofloxacin and tobramycin. Antimicrob. Agents. Chemother. 47, 317-323, 2003.
38. Bassler, B.L., How bacteria talk to each other: regulation of gene expression by quorum sensing. Curr. Opin. Microbiol. 2, 582-587, 1999.
39. Aguilar, C., Friscina, A., Devescovi, G., Kojic, M., and Venturi, V., Identification of quorum-sensing-regulated genes of Burkholderia cepacia. J. Bacteriol. 185, 6456-6462, 2003.
40. Parkins, M.D., Altebaeumer, M., Ceri, H., and Storey, D.G., Subtractive hybridization based identification of genes uniquely or hyperexpressed during biofilm growth. In: Doyle, R.J., ed., Microbial Growth in Biofilms. Methods Enzymol. Vol 336, Part A. San Diego: Academic Press, 2001: 76-84.
41. Beloin, C., Valle, J., Latour-Lambert, P., Faure, P., Kzreminski, M., Balestrino, D., Haagensen, J.A., Molin, S., Prensier, G., Arbeille, B., and Ghigo, J.M., Global impact of mature biofilm lifestyle on Escherichia coli K-12 gene expression. Mol. Microbiol. 51, 659-674, 2004.
42. Whiteley, M., Bangera, M.G., Bumgarner, R.E., Parsek, M.R., Teitzel, G.M., Lory, S., and Greenberg, E.P., Gene expression in Pseudomonas aeruginosa biofilms. Nature 413, 860-864, 2001.
43. Ren, D., Bedzyk, L.A., Thomas, S.M., Ye, R.W., and Wood, T.K., Gene expression in Escherichia coli biofilms. Appl. Microbiol. Biotechnol. 64, 515-524, 2004.
44. Arevalo-Ferro, C., Hentzer, M., Reil, G., Gorg, A., Kjelleberg, S., Givskov, M., Riedel, K., and Eberl, L., Identification of quorum-sensing regulated proteins in the opportunistic pathogen Pseudomonas aeruginosa by proteomics. Environ. Microbiol. 5, 1350-1369, 2003.
45. Sauer, K., The genomics and proteomics of biofilm formation. Genome Biol. 4, 219-229, 2003.
46. Ghigo, J.M., Are there biofilm-specific physiological pathways beyond a reasonable doubt? Res. Microbiol. 154, 1-8, 2003.
47. De Kievit, T.R., Parkins, M.D., Gillis, R.J., Ceri, H., Poole, K., Iglewski, B.H., and Storey, D.G., The contribution of multidrug efflux pumps to the antibiotic resistance of Pseudomonas aeruginosa biofilms. Antimicrob. Agents Chem. 45, 1761-1770, 2001.
48. Spoering, A.L., and Lewis, K., Biofilms and planktonic cells of Pseudomonas aeruginosa have similar resistance to killing by antimicrobials. J. Bacteriol. 183, 6746-6751, 2001.
49. Lewis, K., Riddle of biofilm resistance. Antimicrob. Agents Chemother. 45, 999-1007, 2001.
50. Keren, I., Kaldalu, N., Spoering, A., Wang, Y., and Lewis, K., Persister cells and tolerance to antimicrobials. FEMS Microbiol. Lett. 230, 13-18, 2004.
51. Methods Enzymology, Vol. 336, Microbial Growth in Biofilms, Part A.
52. Wilson, M., Susceptibility of oral bacterial biofilms to antimicrobial agents. J. Med. Microbiol. 44, 79-87, 1996.
53. Lamfon, H., Porter, S.R., McCullough, M., and Pratten, J., Susceptibility of Candida albicans biofilms grown in a constant depth film fermentor to chlorhexidine, fluconazole and miconazole: a longitudinal study. J. Antimicrob. Chemother. 53, 383-385, 2004.
54. Raad, I., Darouiche, R., Hachem, R., Sacilowski, M., and Bodey, G.P., Antibiotics and prevention of microbial colonization of catheters. Antimicrob. Agents Chemother. 39, 2397-2400, 1995.
55. Kumon, H., Ono, N., Iida, M., and Nickel, J.C., Combination effect of fosfomycin and ofloxacin against Pseudomonas aeruginosa growing in a biofilm. Antimicrob. Agents Chemother. 39, 1038-1044, 1995.
56. Ceri, H., Olson, M.E., Morck, D., Storey, D., Read, R.R., Buret, A., and Olson, B., The MBECTTAssay System: multiple equivalent biofilms for antibiotic and biocide susceptibility testing. In: Doyle R.J., ed., Methods Enzymol. Vol. 337, Microbial Growth in Biofilms. Part B. San Diego: Academic Press, 2001:377-385.
57. Bardouniotis, E., Ceri, H., and Olson, M.E., Biofilm formation and biocide susceptibility testing of Mycobacterium fortuitum and Mycobacterium marinum. Cur. Microbiol. 46, 28-32, 2003.
58. Olson, M.E., Ceri, H., Morck, D.W., Buret, A.G., and Read, R.R., Biofilm bacteria: formation and comparative susceptibility to antibiotics. Can. J. Vet. Res. 66, 86-92, 2002.
59. Smith, A.L., Fiel, S.B., Mayer-Hamblett, N., Ramsey, B., and Burns, J.L., Susceptibility testing of Pseudomonas aeruginosa isolates and clinical response to parenteral antibiotic administration Lack of association in cystic fibrosis. Chest 123, 1495-1502, 2003.
60. Sepandj, F., Ceri, H., Gibb, A.P., Read, R.R., and Olson, M.E., Minimum inhibitory concentration (MIC) versus minimum biofilm eliminating concentration (MBEC) in evaluation of antibiotic sensitivity of Gram-negative bacilli causing peritonitis. Peritoneal. Dial. Int. 24, 1-3, 2004.
61. Konig, C., Schwank, S., and Blaser, J., Factors compromising antibiotic activity against biofilms of Staphylococcus epidermidis. Eur. J. Clin. Microbiol. Infect. Dis. 20, 20-26, 2001.
62. Anwar, H., Strap, J.L., and Costerton, J.W., Establishment of aging biofilms: possible mechanism of bacterial resistance to antimicrobial therapy. Antimicrob. Agents Chemother. 36, 1347-1351, 1992.
63. Morck, D.W., Lam, K., McKay, S.G., Olson, M.E., Prosser, B., Ellis, B.D., Cleeland R., and Costerton, J.W., Comparative evaluation of fleroxacin, ampicillin, trimethoprimsulfamethoxazole, and gentamicin as treatments of catheter-associated urinary tract infections in a rabbit model. Int. J. Antimicrobial. Agents 2, S21-S27, 1994.
64. Monzon, M., Garcia-Alvarez, F., Lacleriga, A., Gracia, E., Leiva, J., Oteiza, C., and Amorena, B., A simple infection model using pre-colonized implants to reproduce rat chronic Staphylococcus aureus osteomyelitis and study antibiotic treatment. J. Orthop. Res.19, 820-826, 2001.
65. Nalepa, C.J., Howarth, J.N., Liimatta, E., Ceri, H., Stremick, C.A., Stout, J.E., and Lin, Y.E., The control of bacteria on surfaces: effectiveness of bromine-based biocides toward microbial biofilms and biofilm-associated Legionella pneumophila. CTI J. 24, 12-21, 2003.