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Flavier A.B., Ganova-Raeva L.M., Schell M.A., Denny T.P. Hierarchical autoinduction in Ralstonia solanacearum: control of acyl-homoserine lactone production by a novel autoregulatory system responsive to 3-hydroxy-palmitic acid methyl ester. J Bacteriol. 179:1997;7089-7097.
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0031595961
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s) homologue regulates acylhomoserine lactone-dependent autoinduction in Ralstonia solanacearum
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Transposon mutagenesis was used to identify genes required for homoserine latone (HSL) production in R. solanacearum. The rpoS homologue was identified, and shown to be required for expression of the luxR homologue solR. SolR, in turn, is necessary for expression of the luxI homologue solI. RpoS exerts its effect on SolR-SolI independently of the LysR homologue PhcA. These results demonstrate that multiple regulatory pathways converge to control quorum sensing in R. solanacearum.
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s) homologue regulates acylhomoserine lactone-dependent autoinduction in Ralstonia solanacearum. Mol Microbiol. 28:1998;475-486. Transposon mutagenesis was used to identify genes required for homoserine latone (HSL) production in R. solanacearum. The rpoS homologue was identified, and shown to be required for expression of the luxR homologue solR. SolR, in turn, is necessary for expression of the luxI homologue solI. RpoS exerts its effect on SolR-SolI independently of the LysR homologue PhcA. These results demonstrate that multiple regulatory pathways converge to control quorum sensing in R. solanacearum.
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Flavier, A.B.1
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19
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Octopine-type Ti plasmids code for a mannopine-inducible dominant-negative allele of traR, the quorum-sensing activator that regulates Ti plasmid conjugal transfer
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Oger P., Kim K.-S., Sackett R.L., Piper K.R., Farrand S.K. Octopine-type Ti plasmids code for a mannopine-inducible dominant-negative allele of traR, the quorum-sensing activator that regulates Ti plasmid conjugal transfer. Mol Microbiol. 27:1998;277-288.
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20
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0031963471
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Activity of the quorum-sensing regulator TraR of Agrobacterium tumefaciens is inhibited by a truncated, dominant defective TraR-like protein
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This report shows that a TraR-like protein called TraS is encoded on the A. tumefaciens virulence plasmid. TraS lacks a DNA-binding domain and acts as a dominant negative inhibitor of TraR function presumably by forming inactive mixed TraR-TraS oligomers. Expression of traS is induced by the plant hormone mannopine, which results in inhibition of TraR-mediated conjugation.
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Zhu J., Winans S.C. Activity of the quorum-sensing regulator TraR of Agrobacterium tumefaciens is inhibited by a truncated, dominant defective TraR-like protein. Mol Microbiol. 27:1998;289-297. This report shows that a TraR-like protein called TraS is encoded on the A. tumefaciens virulence plasmid. TraS lacks a DNA-binding domain and acts as a dominant negative inhibitor of TraR function presumably by forming inactive mixed TraR-TraS oligomers. Expression of traS is induced by the plant hormone mannopine, which results in inhibition of TraR-mediated conjugation.
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Zhu, J.1
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G.M. Dunny, & S.C. Winans. Washington DC: ASM Press
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Winans S.C., Zhu J., More M.I. Cell density-dependent gene expression by Agrobacterium tumefaciens during colonization of crown gall tumors. Dunny G.M., Winans S.C. Cell-Cell Signaling in Bacteria. 1999;117-128 ASM Press, Washington DC.
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Winans, S.C.1
Zhu, J.2
More, M.I.3
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22
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Integration of the quorum-sensing system in the regulatory networks controlling virulence factor synthesis in Erwinia chrysanthemi
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Reverchon S., Bouillant M.L., Salmond G., Nasser W. Integration of the quorum-sensing system in the regulatory networks controlling virulence factor synthesis in Erwinia chrysanthemi. Mol Microbiol. 29:1998;1407-1418.
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Evans K., Passador L., Srikumar R., Tsang E., Nezezon J., Poole K. Influence of the MexAB-OprM multidrug efflux system on quorum sensing in Pseudomonas aeruginosa. J Bacteriol. 180:1998;5443-5447.
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24
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Production of acyl-homoserine lactone quorum-sensing signals by Gram-negative plant-associated bacteria
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Cha C., Gao P., Chen Y.-C., Shaw P.D., Farrand S.K. Production of acyl-homoserine lactone quorum-sensing signals by Gram-negative plant-associated bacteria. Mol Plant-Microbe Interactions. 11:1998;1119-1129.
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25
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0002030947
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Cell-density sensing during early development in Myxococcus xanthus
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G.M. Dunny, & S.C. Winans. Washington DC: ASM Press
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Plamann L., Kaplan H.B. Cell-density sensing during early development in Myxococcus xanthus. Dunny G.M., Winans S.C. Cell-Cell Signaling in Bacteria. 1999;67-82 ASM Press, Washington DC.
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Plamann, L.1
Kaplan, H.B.2
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26
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0002471490
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Cell contact-dependent C signaling in Myxococcus xanthus
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G.M. Dunny, & S.C. Winans. Washington DC: ASM Press
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Shimkets L.J., Kaiser D. Cell contact-dependent C signaling in Myxococcus xanthus. Dunny G.M., Winans S.C. Cell-Cell Signaling in Bacteria. 1999;83-100 ASM Press, Washington DC.
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Shimkets, L.J.1
Kaiser, D.2
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27
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0030814729
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Quorum sensing by peptide pheromones and two-component signal-transduction systems in Gram-positive bacteria
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Kleerebezem M., Quadri L.E.N., Kuipers O.P., de Vos W.M. Quorum sensing by peptide pheromones and two-component signal-transduction systems in Gram-positive bacteria. Mol Microbiol. 24:1997;895-904.
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28
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Virulence gene regulation by peptides in staphylococci and other Gram-positive bacteria
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Novick R.P., Muir T.W. Virulence gene regulation by peptides in staphylococci and other Gram-positive bacteria. Curr Opin Microbiol. 2:1999;40-45.
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Muir, T.W.2
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30
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0032538886
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Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor
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This study presents both in vivo and in vitro evidence that accumulation of the B. subtilis competence regulator ComK is controlled by MecA/ClpP driven proteolysis. The experiments show that the ComS protein, which is synthesised in response to the ComX and CSF quorum sensing peptides, allows ComK to dissociate from a complex with MecA and ClpP and escape degradation. The results demonstrate that this is a critical step in competence development.
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Turgay K., Hahn J., Burghoorn J., Dubnau D. Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor. EMBO J. 17:1998;6730-6738. This study presents both in vivo and in vitro evidence that accumulation of the B. subtilis competence regulator ComK is controlled by MecA/ClpP driven proteolysis. The experiments show that the ComS protein, which is synthesised in response to the ComX and CSF quorum sensing peptides, allows ComK to dissociate from a complex with MecA and ClpP and escape degradation. The results demonstrate that this is a critical step in competence development.
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EMBO J
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Turgay, K.1
Hahn, J.2
Burghoorn, J.3
Dubnau, D.4
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31
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0002590055
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Sex pheromone systems in Enterococci
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G.M. Dunny, & S.C. Winans. Washington DC: ASM Press
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Clewell D.B. Sex pheromone systems in Enterococci. Dunny G.M., Winans S.C. Cell-Cell Signaling in Bacteria. 1999;47-66 ASM Press, Washington DC.
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Clewell, D.B.1
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32
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0032568644
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Regulation of the pAD1 sex pheromone response of Enterococcus faecalis by direct interaction between the cAD1 peptide mating signal and the negatively regulating DNA-binding TraA protein
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Fujimoto S., Clewell D.B. Regulation of the pAD1 sex pheromone response of Enterococcus faecalis by direct interaction between the cAD1 peptide mating signal and the negatively regulating DNA-binding TraA protein. Proc Natl Acad Sci USA. 95:1998;6430-6435.
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33
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0345007912
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Regulation of the Enterococcus faecalis pAD1-related sex pheromone response: Analyses of traD expression and its role in controlling conjugation functions
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do Carmo de Freire Bastos M., Tomita H., Tanimoto K., Clewell D.B. Regulation of the Enterococcus faecalis pAD1-related sex pheromone response: analyses of traD expression and its role in controlling conjugation functions. Mol Microbiol. 30:1998;381-392.
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Do Carmo De Freire Bastos, M.1
Tomita, H.2
Tanimoto, K.3
Clewell, D.B.4
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34
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0032103979
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Cloning and characterization of an accessory gene regulator (agr)-like locus from Staphylococcus epidermidis
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Van Wamel W.J.B., van Rossum G., Verhoef J., Vandenbroucke-Grauls C.M.J.E., Fluit A.C. Cloning and characterization of an accessory gene regulator (agr)-like locus from Staphylococcus epidermidis. FEMS Mircobiol Lett. 163:1998;1-9.
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Van Wamel, W.J.B.1
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Vandenbroucke-Grauls, C.M.J.E.4
Fluit, A.C.5
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35
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Structure of the pheromone peptide of the Staphylococcus epidermidis agr system
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Otto M., Sussmuth R., Jung G., Gotz F. Structure of the pheromone peptide of the Staphylococcus epidermidis agr system. FEBS Lett. 424:1998;89-94.
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36
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0031959577
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Transmembrane topology and histidine protein kinase activity of AgrC, the agr signal receptor in Staphylococcus aureus
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Lina G., Jarraud S., Ji G., Greenland T., Pedraza A., Etienne J., Novick R.P., Vandenesch F. Transmembrane topology and histidine protein kinase activity of AgrC, the agr signal receptor in Staphylococcus aureus. Mol Microbiol. 28:1998;655-662.
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Lina, G.1
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Etienne, J.6
Novick, R.P.7
Vandenesch, F.8
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37
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0027247722
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Intercellular signalling in Vibrio harveyi: Sequence and function of genes regulating expression of luminescence
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Bassler B.L., Wright M., Showalter M.E., Silverman M.R. Intercellular signalling in Vibrio harveyi: sequence and function of genes regulating expression of luminescence. Mol Microbiol. 9:1993;773-786.
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Bassler, B.L.1
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Silverman, M.R.4
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38
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0028228175
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Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: Sequence and function of genes encoding a second sensory pathway
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Bassler B.L., Wright M., Silverman M.R. Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathway. Mol Microbiol. 13:1994;273-286.
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Mol Microbiol
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Bassler, B.L.1
Wright, M.2
Silverman, M.R.3
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39
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0028242863
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Sequence and function of luxO, a negative regulator of luminescence in Vibrio harveyi
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Bassler B.L., Wright M., Silverman M.R. Sequence and function of luxO, a negative regulator of luminescence in Vibrio harveyi. Mol Microbiol. 12:1994;403-412.
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Mol Microbiol
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Bassler, B.L.1
Wright, M.2
Silverman, M.R.3
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40
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0000827274
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A multichannel two-component signaling relay controls quorum sensing in Vibrio harveyi
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G.M. Dunny, & S.C. Winans. Washington DC: ASM Press
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Bassler B.L. A multichannel two-component signaling relay controls quorum sensing in Vibrio harveyi. Dunny G.M., Winans S.C. Cell-Cell Signaling in Bacteria. 1999;259-273 ASM Press, Washington DC.
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Bassler, B.L.1
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41
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0032927738
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A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi
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5-fold range.
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5-fold range.
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Mol Microbiol
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Freeman, J.A.1
Bassler, B.L.2
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42
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0032917463
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Sequence and function of LuxU: A two component phosphorelay protein that regulates quorum sensing in Vibrio harveyi
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In this work, a new member of the V. harveyi Lux signal relay, LuxU, is identified and analysed. This paper presents the cloning, sequencing and mutagenesis of the LuxU phosphorelay protein. Importantly, the results demonstrate that the function of LuxU is to couple signals emanating from the two autoinducer sensors (LuxN and LuxQ) to the response regulator LuxO.
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Freeman J.A., Bassler B.L. Sequence and function of LuxU: a two component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. J Bacteriol. 181:1999;899-906. In this work, a new member of the V. harveyi Lux signal relay, LuxU, is identified and analysed. This paper presents the cloning, sequencing and mutagenesis of the LuxU phosphorelay protein. Importantly, the results demonstrate that the function of LuxU is to couple signals emanating from the two autoinducer sensors (LuxN and LuxQ) to the response regulator LuxO.
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(1999)
J Bacteriol
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Freeman, J.A.1
Bassler, B.L.2
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43
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0033574064
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Quorum sensing in Escherichia coli, Salmonella typhimurium and Vibrio harveyi: A new family of genes involved in autoinducer production
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This important work presents the identification and analysis of the luxS gene and demonstrates that it is responsible for AI-2 production in V. harveyi, E. coli and S. typhimurium. The paper further shows that very highly conserved luxS homologues are widespread in both Gram-negative and Gram-positive bacteria. These results imply that AI-2 signalling could be a universal cell-cell communication system.
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Surette M.G., Miller M.B., Bassler B.L. Quorum sensing in Escherichia coli, Salmonella typhimurium and Vibrio harveyi: a new family of genes involved in autoinducer production. Proc Natl Acad Sci USA. 96:1999;1639-1644. This important work presents the identification and analysis of the luxS gene and demonstrates that it is responsible for AI-2 production in V. harveyi, E. coli and S. typhimurium. The paper further shows that very highly conserved luxS homologues are widespread in both Gram-negative and Gram-positive bacteria. These results imply that AI-2 signalling could be a universal cell-cell communication system.
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Proc Natl Acad Sci USA
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Surette, M.G.1
Miller, M.B.2
Bassler, B.L.3
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44
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Quorum sensing in Escherichia coli and Salmonella typhimurium
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This paper presents the first convincing evidence for quorum sensing in E. coli and S. typhimurium. V. harveyi reporter strains that specifically respond to either AI-1 or AI-2 are used to show that E. coli and S. typhimurium produce an AI-2-like activity. Furthermore, this report shows that signal production in E. coli and S. typhimurium is highly regulated, responsive to a number of environmental cues, and autoinducer activity is degraded as the cells enter stationary phase. This is the first report of degradation of an autoinducer.
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Surette M.G., Bassler B.L. Quorum sensing in Escherichia coli and Salmonella typhimurium. Proc Natl Acad Sci USA. 95:1998;7046-7050. This paper presents the first convincing evidence for quorum sensing in E. coli and S. typhimurium. V. harveyi reporter strains that specifically respond to either AI-1 or AI-2 are used to show that E. coli and S. typhimurium produce an AI-2-like activity. Furthermore, this report shows that signal production in E. coli and S. typhimurium is highly regulated, responsive to a number of environmental cues, and autoinducer activity is degraded as the cells enter stationary phase. This is the first report of degradation of an autoinducer.
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(1998)
Proc Natl Acad Sci USA
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Surette, M.G.1
Bassler, B.L.2
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Regulation of autoinducer production in Salmonella typhimurium
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Surette M.G., Bassler B.L. Regulation of autoinducer production in Salmonella typhimurium. Mol Microbiol. 31:1999;585-595.
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Mol Microbiol
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Cross-species induction of luminescence in the quorum sensing bacterium Vibrio harveyi
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Bassler B.L., Greenberg E.P., Stevens A.M. Cross-species induction of luminescence in the quorum sensing bacterium Vibrio harveyi. J Bacteriol. 179:1997;4043-4045.
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Stevens, A.M.3
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Staphylococcus aureus Agr and Sar global regulators influence internalization and induction of apoptosis
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Wesson C.A., Liou L.E., Todd K.M., Bohach G.A., Trumble W.R., Bayles K.W. Staphylococcus aureus Agr and Sar global regulators influence internalization and induction of apoptosis. Infect Immun. 66:1998;5238-5243.
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Wesson, C.A.1
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Bayles, K.W.6
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Ji G., Beavis R., Novick R.P. Bacterial interference caused by autoinducing peptide variants. Science. 276:1997;2027-2030.
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Ji, G.1
Beavis, R.2
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49
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Structure-activity analysis of synthetic autoinducing thiolactone peptides from Staphylococcus aureus responsible for virulence
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The structure of the S. aureus autoinducer is reported. The autoinducers are shown to be cyclic thiolactone peptides. In a continuation of the studies begun in [48], the present work offers evidence that the thiolactone is required for induction but not inhibition of agr expression.
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Mayville P., Ji G., Beavis R., Yang H., Goger M., Novick R.P., Muir T.W. Structure-activity analysis of synthetic autoinducing thiolactone peptides from Staphylococcus aureus responsible for virulence. Proc Natl Acad Sci USA. 96:1999;1218-1223. The structure of the S. aureus autoinducer is reported. The autoinducers are shown to be cyclic thiolactone peptides. In a continuation of the studies begun in [48], the present work offers evidence that the thiolactone is required for induction but not inhibition of agr expression.
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Proc Natl Acad Sci USA
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Mayville, P.1
Ji, G.2
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Yang, H.4
Goger, M.5
Novick, R.P.6
Muir, T.W.7
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Allison D.G., Ruiz B., SanJose C., Jaspe A., Gilbert P. Extracellular products as mediators of the formation and detachment of Pseudomonas fluorescens biofilms. FEMS Microbiol Lett. 167:1998;179-184.
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Ruiz, B.2
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Gilbert, P.5
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52
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Costerton J.W., Lewandowski Z., Caldwell D.E., Korber D.R., Lappin-Scott H.M. Microbial biofilms. Ornston L.N., Balows A., Greenberg E.P. Annual Review of Microbiology. 1995;711-745 Annual Reviews, Palo Alto CA.
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Lewandowski, Z.2
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53
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Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: A genetic analysis
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O'Toole G.A., Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol. 28:1998;449-461.
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Interpopulation signaling via N-acyl-homoserine lactones among bacteria in the wheat rhizosphere
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Naturally occurring wheat-rhizosphere associated bacteria are shown to restore phenazine production to a P. aureofaciens autoinducer mutant. This result implies that cross-feeding of quorum sensing autoinducers occurs in natural soil environments.
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Pierson E.A., Wood D.W., Cannon J.A., Blachere F.M., Pierson L.S.I.I.I. Interpopulation signaling via N-acyl-homoserine lactones among bacteria in the wheat rhizosphere. Mol Plant-Microbe Interactions. 11:1998;1078-1084. Naturally occurring wheat-rhizosphere associated bacteria are shown to restore phenazine production to a P. aureofaciens autoinducer mutant. This result implies that cross-feeding of quorum sensing autoinducers occurs in natural soil environments.
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Mol Plant-Microbe Interactions
, vol.11
, pp. 1078-1084
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Pierson, E.A.1
Wood, D.W.2
Cannon, J.A.3
Blachere, F.M.4
Pierson, L.S.I.I.I.5
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55
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N-acyl-L-homoserine lactone autoinducers control production of an extracellular lipopeptide biosurfactant required for swarming motility of Serratia liquefaciens MG1
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Lindum P.W., Anthoni U., Christophersen C., Eberl L., Molin S., Givskov M. N-acyl-L-homoserine lactone autoinducers control production of an extracellular lipopeptide biosurfactant required for swarming motility of Serratia liquefaciens MG1. J Bacteriol. 180:1998;6384-6388.
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J Bacteriol
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Lindum, P.W.1
Anthoni, U.2
Christophersen, C.3
Eberl, L.4
Molin, S.5
Givskov, M.6
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56
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Two separate regulatory systems participate in control of swarming motility of Serratia liquefaciens MG1
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Givskov M., Ostling J., Eberl L., Lindum P.W., Beck Christensen A., Christiansen G., Molin S., Kjelleberg S. Two separate regulatory systems participate in control of swarming motility of Serratia liquefaciens MG1. J Bacteriol. 180:1998;742-745.
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J Bacteriol
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Givskov, M.1
Ostling, J.2
Eberl, L.3
Lindum, P.W.4
Beck Christensen, A.5
Christiansen, G.6
Molin, S.7
Kjelleberg, S.8
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57
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0029854171
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Eukaryotic interference with homoserine lactone-mediated prokaryotic signalling
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Givskov M., de Nys R., Manefield M., Gram L., Maximilien R., Eberl L., Molin S., Steinberg P.D., Kjelleberg S. Eukaryotic interference with homoserine lactone-mediated prokaryotic signalling. J Bacteriol. 178:1996;6618-6622.
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Givskov, M.1
De Nys, R.2
Manefield, M.3
Gram, L.4
Maximilien, R.5
Eberl, L.6
Molin, S.7
Steinberg, P.D.8
Kjelleberg, S.9
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58
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0033060727
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Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein
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In an extension of the work presented in [57], this paper presents evidence that the halogenated furanones produced by D. pulchra inhibit quorum sensing by competing for the homoserine-lactone (HSL) autoinducer-binding site in LuxR. Furanone-mediated inhibition of light production was monitored in an E. coli strain harbouring the V. fischeri luxR and luxCDABE genes. Furanones were further tested for the ability to displace radiolabelled V. fischeri autoinducer from E. coli cells overproducing LuxR. This important work, along with [57], suggests that one defensive strategy employed by eukaryotes to avoid colonisation by bacteria is to specifically target and inhibit quorum sensing controlled functions.
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Manefield M., de Nys R., Kumar N., Read R., Givskov M., Steinberg P., Kjelleberg S. Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein. Microbiology. 145:1999;283-291. In an extension of the work presented in [57], this paper presents evidence that the halogenated furanones produced by D. pulchra inhibit quorum sensing by competing for the homoserine-lactone (HSL) autoinducer-binding site in LuxR. Furanone-mediated inhibition of light production was monitored in an E. coli strain harbouring the V. fischeri luxR and luxCDABE genes. Furanones were further tested for the ability to displace radiolabelled V. fischeri autoinducer from E. coli cells overproducing LuxR. This important work, along with [57], suggests that one defensive strategy employed by eukaryotes to avoid colonisation by bacteria is to specifically target and inhibit quorum sensing controlled functions.
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(1999)
Microbiology
, vol.145
, pp. 283-291
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Manefield, M.1
De Nys, R.2
Kumar, N.3
Read, R.4
Givskov, M.5
Steinberg, P.6
Kjelleberg, S.7
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