A modified serine cycle in Escherichia coli coverts methanol and CO2 to two-carbon compounds

Nature Communications

Volumn 9, Issue 1, 2018, Pages

  Yu, Hong ^a,b   Liao, James C ^c  

^a University of California   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c ACADEMIA SINICA   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI;

ALCOHOL; CARBON DIOXIDE; ESCHERICHIA COLI PROTEIN; METHANOL; SERINE;

ESCHERICHIA COLI; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

CARBON DIOXIDE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; ETHANOL; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; METHANOL; SERINE;

EID: 85054089047     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/s41467-018-06496-4     Document Type: Article

References (26)

1. Sawatdeenarunat, C. et al. Anaerobic biorefinery: current status, challenges, and opportunities. Bioresour. Technol. 215, 304–313 (2016)
2. Caballero, A. & Pérez, P. J. Methane as raw material in synthetic chemistry: the final frontier. Chem. Soc. Rev. 42, 8809–8820 (2013)
3. 2 to higher alcohols. Science 335, 1596 (2012)
4. Jönsson, L. J. & Martín, C. Pretreatment of lignocellulose: formation of inhibitory by-products and strategies for minimizing their effects. Bioresour. Technol. 199, 103–112 (2016)
5. Kozlowski, J. T. & Davis, R. J. Heterogeneous catalysts for the Guerbet coupling of alcohols. ACS Catal. 3, 1588–1600 (2013)
6. Smejkalová, H., Erb, T. J. & Fuchs, G. Methanol assimilation in Methylobacterium extorquens AM1: demonstration of all enzymes and their regulation. PLoS ONE 5, pii: e13001 (2010)
7. Whitaker, W. B. et al. Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli. Metab. Eng. 39, 49–59 (2017)
8. Meyer, F. et al. Methanol-essential growth of Escherichia coli. Nat. Commun. 9, 1508 (2018)
9. Woolston, B. M., King, J. R., Reiter, M., Van Hove, B. & Stephanopoulos, G. Improving formaldehyde consumption drives methanol assimilation in engineered E. coli. Nat. Commun. 9, 2387 (2018)
10. Bogorad, I. W. et al. Building carbon-carbon bonds using a biocatalytic methanol condensation cycle. Proc. Natl. Acad. Sci. USA 111, 15928–15933 (2014)
11. Crowther, G. J., Kosály, G. & Lidstrom, M. E. Formate as the main branch point for methylotrophic metabolism in Methylobacterium extorquens AM1. J. Bacteriol. 190, 5057–5062 (2008)
12. Kalyuzhnaya, M. G. & Lidstrom, M. E. QscR-mediated transcriptional activation of serine cycle genes in Methylobacterium extorquens AM1. J. Bacteriol. 187, 7511–7517 (2005)
13. Ravnikar, P. D. & Somerville, R. L. Genetic characterization of a highly efficient alternate pathway of serine biosynthesis in Escherichia coli. J. Bacteriol. 169, 2611–2617 (1987)
14. Huo, Y. X. et al. Conversion of proteins into biofuels by engineering nitrogen flux. Nat. Biotechnol. 29, 346–351 (2011)
15. Nuñez, M. F., Pellicer, M. T., Badia, J., Aguilar, J. & Baldoma, L. Biochemical characterization of the 2-ketoacid reductases encoded by ycdW and yiaE genes in Escherichia coli. Biochem. J. 354, 707–715 (2001)
16. Mainguet, S. E., Gronenberg, L. S., Wong, S. S. & Liao, J. C. A reverse glyoxylate shunt to build a non-native route from C4 to C2 in Escherichia coli. Metab. Eng. 19, 116–1127 (2013)
17. Giffin, M. M., Modesti, L., Raab, R. W., Wayne, L. G. & Sohaskey, C. D. ald of Mycobacterium tuberculosis encodes both the alanine dehydrogenase and the putative glycine dehydrogenase. J. Bacteriol. 194, 1045–1054 (2012)
18. Grabowski, R., Hofmeister, A. E. & Buckel, W. Bacterial L-serine dehydratases: a new family of enzymes containing iron-sulfur clusters. Trends Biochem. Sci. 18, 297–300 (1993)
19. Okamura-Ikeda, K., Ohmura, Y., Fujiwara, K. & Motokawa, Y. Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system. Eur. J. Biochem. 216, 539–548 (1993)
20. Yishai, O., Goldbach, L., Tenenboim, H., Lindner, S. N. & Bar-Even, A. Engineered assimilation of exogenous and endogenous formate in Escherichia coli. ACS Synth. Biol. 6, 1722–1731 (2017)
21. Wu, T. Y. et al. Characterization and evolution of an activator-independent methanol dehydrogenase from Cupriavidus necator N-1. Appl. Microbiol. Biotechnol. 100, 4969–4983 (2016)
22. Peters-Wendisch, P. G. et al. Pyruvate carboxylase from Corynebacterium glutamicum: characterization, expression and inactivation of the pyc gene. Microbiology 144, 915–927 (1998)
23. Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343–345 (2009)
24. Petrarulo, Michele et al. High-performance liquid chromatographic determination of glyoxylic acid in urine. J. Chromatogr. B Biomed. Sci. Appl. 432, 37–46 (1998)
25. Han, J., Gagnon, S., Eckle, T. & Borchers, C. H. Metabolomic analysis of key central carbon metabolism carboxylic acids as their 3-nitrophenylhydrazones by UPLC/ESI-MS. Electrophoresis 34, 2891–2900 (2013)
26. Lin, P. P. et al. Construction and evolution of an Escherichia coli strain relying on nonoxidative glycolysis for sugar catabolism. Proc. Natl. Acad. Sci. USA 115, 3538–3546 (2018)