A reverse glyoxylate shunt to build a non-native route from C4 to C2 in Escherichia coli

Metabolic Engineering

Volumn 19, Issue , 2013, Pages 116-127

  Mainguet, Samuel E ^a   Gronenberg, Luisa S ^a   Wong, Sio Si ^a   Liao, James C ^a,b  

^a University of California   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Acetyl coA biosynthesis; Central metabolism; Glyoxylate shunt; Non native pathways; Primary metabolism; Reversibility

Indexed keywords

ACETYL-COA; CENTRAL METABOLISMS; GLYOXYLATE; NON-NATIVE; REVERSIBILITY;

BIOCHEMISTRY; CARBON; CARBON DIOXIDE; CARBOXYLATION; ESCHERICHIA COLI; MOLECULES; PHYSIOLOGY;

METABOLISM;

ACETYL COENZYME A; ADENOSINE TRIPHOSPHATE; CARBON; CITRIC ACID; GLYOXYLIC ACID; ISOCITRATE LYASE; MALATE SYNTHASE; MALIC ACID; OXALOACETIC ACID; SUCCINIC ACID;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; CARBON SOURCE; CITRIC ACID CYCLE; CONTROLLED STUDY; ESCHERICHIA COLI; MOLECULE; NONHUMAN; PRIORITY JOURNAL; THERMODYNAMICS;

ESCHERICHIA COLI;

ACETYL-COA BIOSYNTHESIS; CENTRAL METABOLISM; GLYOXYLATE SHUNT; NON-NATIVE PATHWAYS; PRIMARY METABOLISM; REVERSIBILITY;

ACETYL COENZYME A; CITRIC ACID CYCLE; ESCHERICHIA COLI; GLUCOSE; GLYOXYLATES; MALATES; METABOLIC ENGINEERING; OXALOACETIC ACID; SUCCINIC ACID;

EID: 84883554005     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2013.06.004     Document Type: Article

References (27)

1. Alberty R.A. Biochemical thermodynamics: applications of mathematica. Methods Biochem. Anal. 2006, 48:1-458.
2. Ashworth J.M., Kornberg H.L. The anaplerotic fixation of carbon dioxide by Escherichia coli. Proc. R. Soc. London B. Biol. Sci. 1966, 165:179-188.
3. Atsumi S., Cann A.F., Connor M.R., Shen C.R., Smith K.M., Brynildsen M.P., Chou K.J., Hanai T., Liao J.C. Metabolic engineering of Escherichia coli for 1-butanol production. Metab. Eng. 2008, 10:305-311.
4. Baba T., Ara T., Hasegawa M., Takai Y., Okumura Y., Baba M., Datsenko K.A., Tomita M., Wanner B.L., Mori H. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2006, 2:0008. 2006.
5. Bar-Even A., Noor E., Milo R. A survey of carbon fixation pathways through a quantitative lens. J. Exp. Bot. 2012, 63:2325-2342.
6. Berg I.A. Ecological aspects of the distribution of different autotrophic CO2 fixation pathways. Appl. Environ. Microbiol. 2011, 77:1925-1936.
7. Chistoserdova L., Lidstrom M.E. Molecular and mutational analysis of a DNA region separating two methylotrophy gene clusters in Methylobacterium extorquens AM1. Microbiology 1997, 143(Pt 5):1729-1736.
8. Chistoserdova L.V., Lidstrom M.E. Genetics of the serine cycle in Methylobacterium extorquens AM1: identification of sgaA and mtdA and sequences of sgaA, hprA, and mtdA. J. Bacteriol. 1994, 176:1957-1968.
9. Cozzone A.J. Regulation of acetate metabolism by protein phosphorylation in enteric bacteria. Annu. Rev. Microbiol. 1998, 52:127-164.
10. Davies S.J., Golby P., Omrani D., Broad S.A., Harrington V.L., Guest J.R., Kelly D.J., Andrews S.C. Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli. J. Bacteriol. 1999, 181:5624-5635.
11. Erb T.J., Frerichs-Revermann L., Fuchs G., Alber B.E. The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and (3S)- Malyl-CoA thioesterase. J. Bacteriol. 2010, 192:1249-1258.
12. Fatland B.L., Ke J., Anderson M.D., Mentzen W.I., Cui L.W., Allred C.C., Johnston J.L., Nikolau B.J., Wurtele E.S. Molecular characterization of a heteromeric ATP-citrate lyase that generates cytosolic acetyl-coenzyme A in Arabidopsis. Plant Physiol. 2002, 130:740-756.
13. Gibson D.G., Young L., Chuang R.Y., Venter J.C., Hutchison C.A., Smith H.O. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 2009, 6:343-345.
14. Groeneveld M., Weme R.G., Duurkens R.H., Slotboom D.J. Biochemical characterization of the C4-dicarboxylate transporter DctA from Bacillus subtilis. J. Bacteriol. 2010, 192:2900-2907.
15. Gruer M.J., Guest J.R. Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli. Microbiology 1994, 140(Pt 10):2531-2541.
16. Hanson R.S., Hanson T.E. Methanotrophic bacteria. Microbiol. Rev. 1996, 60:439-+.
17. Houston B., Nimmo H.G. Purification and some kinetic properties of rat liver ATP citrate lyase. Biochem. J. 1984, 224:437-443.
18. 2 fixation via the reductive tricarboxylic acid cycle in different lineages within the phylum Aquificae: evidence for two ways of citrate cleavage. Environ. Microbiol. 2007, 9:81-92.
19. Huo Y.X., Cho K.M., Rivera J.G., Monte E., Shen C.R., Yan Y., Liao J.C. Conversion of proteins into biofuels by engineering nitrogen flux. Nat. Biotechnol. 2011, 29:346-351.
20. Iuchi S., Kuritzkes D.R., Lin E.C. Three classes of Escherichia coli mutants selected for aerobic expression of fumarate reductase. J. Bacteriol. 1986, 168:1415-1421.
21. Jones H.M., Gunsalus R.P. Transcription of the Escherichia coli fumarate reductase genes (frdABCD) and their coordinate regulation by oxygen, nitrate, and fumarate. J. Bacteriol. 1985, 164:1100-1109.
22. Kim W., Tabita F.R. Both subunits of ATP-citrate lyase from Chlorobium tepidum contribute to catalytic activity. J. Bacteriol. 2006, 188:6544-6552.
23. Kornberg H.L., Krebs H.A. Synthesis of cell constituents from C2-units by a modified tricarboxylic acid cycle. Nature 1957, 179:988-991.
24. Maloy S.R., Nunn W.D. Genetic regulation of the glyoxylate shunt in Escherichia coli K-12. J. Bacteriol. 1982, 149:173-180.
25. Shimamoto T., Izawa H., Daimon H., Ishiguro N., Shinagawa M., Sakano Y., Tsuda M., Tsuchiya T. Cloning and nucleotide sequence of the gene (citA) encoding a citrate carrier from Salmonella typhimurium. J. Biochem. 1991, 110:22-28.
26. Tseng C.P., Yu C.C., Lin H.H., Chang C.Y., Kuo J.T. Oxygen- and growth rate-dependent regulation of Escherichia coli fumarase (FumA, FumB, and FumC) activity. J. Bacteriol. 2001, 183:461-467.
27. Ward N., Larsen O., Sakwa J., Bruseth L., Khouri H., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M., Nelson K.E., Methe B., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D., Ravel J., Tettelin H., Ren Q., Read T., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I., Eidhammer I., Jonasen I., Vanaken S., Utterback T., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A. Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath). PLoS Biology 2004, 2:e303.