High-yield isolation and subcellular proteomic characterization of nuclear and subnuclear structures from trypanosomes

Methods in Molecular Biology

Volumn 463, Issue , 2008, Pages 77-92

  Degrasse, Jeffrey A ^a   Chait, Brian T ^a   Field, Mark C ^b   Rout, Michael P ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Nuclear envelope; Nuclear isolation; Nuclear pore complex; Nucleolus; Proteomics; Subcellular fractionation; Sucrose density gradient centrifugation; Trypanosoma brucei

Indexed keywords

ANIMALIA; EXCAVATA; FUNGI/METAZOA GROUP; GIARDIA; KINETOPLASTIDA; PROTISTA; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

HYDROXYAPATITE;

ANIMAL; BIOCHEMISTRY; BIOLOGICAL MODEL; CELL FRACTIONATION; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; NUCLEAR PORE; NUCLEOLUS; PROCEDURES; PROTEOMICS; TRYPANOSOMA;

ANIMALS; BIOCHEMISTRY; CELL NUCLEOLUS; CELL NUCLEUS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DURAPATITE; MASS SPECTROMETRY; MODELS, BIOLOGICAL; NUCLEAR ENVELOPE; NUCLEAR PORE; PROTEOMICS; SUBCELLULAR FRACTIONS; TRYPANOSOMA;

EID: 85052549634     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-406-3_6     Document Type: Article

References (16)

1. Barrett, M. P., Burchmore, R. J. S., Stich, A., Lazzari, J. O., Frasch, A. C., Cazzulo, J. J., and Krishna, S. (2003) The trypanosomiases. Lancet 362, 1469-1480.
2. The World Health Report 2004-Changing History. (2004) World Health Organization. Geneva.
3. Simpson, A. G., Stevens, J. R., and Lukes, J. (2006) The evolution and diversity of kinetoplastid flagellates. Trends Parasitol. 22, 168-174.
4. Bapteste, E., Charlebois, R. L., Macleod, D., and Brochier, C. (2005) The two tempos of nuclear pore complex evolution: highly adapting proteins in an ancient frozen structure. Genome Biol. 6, R85.
5. Mans, B. J., Anantharaman, V., Aravind, L., and Koonin, E. V. (2004) Comparative genomics, evolution and origins of the nuclear envelope and nuclear pore complex. Cell Cycle 3, 1612-1637.
6. El-Sayed, N. M., Myler, P. J., Blandin, G., Berriman, M., Crabtree, J., Aggarwal, G., Caler, E., Renauld, H., Worthey, E. A., Hertz-Fowler, C., Ghedin, E., Peacock, C., Bartholomeu, D. C., Haas, B. J., Tran, A. N., Wortman, J. R., Alsmark, U. C. M., Angiuoli, S., Anupama, A., Badger, J., Bringaud, F., Cadag, E., Carlton, J. M., Cerqueira, G. C., Creasy, T., Delcher, A. L., Djikeng, A., Embley, T. M., Hauser, C., Ivens, A. C., Kummerfeld, S. K., Pereira-Leal, J. B., Nilsson, D., Peterson, J., Salzberg, S. L., Shallom, J., Silva, J. C., Sundaram, J., Westenberger, S., White, O., Metville, S. E., Donelson, J. E., Andersson, B., Stuart, K. D., and Hall, N. (2005) Comparative genomics of trypanosomatid parasitic protozoa. Science 309, 404-409.
7. Subramaniam, C., Veazey, P., Seth, R., Hayes-Sinclair, J., Chambers, E., Carrington, M., Gull, K., Matthews, K., Horn, D., and Field, M. C. (2006) Chromosome-wide analysis of gene function by RNA interference in the African trypanosome. Eukaryot. Cell 5, 1539-1549.
8. Clayton, C. E. (2002) Life without transcriptional control? From fly to man and back again. EMBO J. 21, 1881-1888.
9. Field, M. C., Horn, D., and Carrington, M. (2008) Analysis of small GTPase function in trypanosomes. In: Small GTPases in disease (Balch, W., Der, C., Hall A., eds.). Academic Press, San Diego, CA.
10. Ullu, E., Tschudi, C., and Chakraborty, T. (2004) RNA interference in protozoan parasites. Cell. Microbiol. 6, 509-519.
11. Berriman, M., Ghedin, E., Hertz-Fowler, C., Blandin, G., Renauld, H., Bartholomeu, D. C., Lennard, N. J., Caler, E., Hamlin, N. E., Haas, B., Bohme, W., Hannick, L., Aslett, M. A., Shallom, J., Marcello, L., Hou, L. H., Wickstead, B., Alsmark, U. C. M., Arrowsmith, C., Atkin, R. J., Barron, A. J., Bringaud, F., Brooks, K., Carrington, M., Cherevach, I., Chillingworth, T. J., Churcher, C., Clark, L. N., Corton, C. H., Cronin, A., Davies, R. M., Doggett, J., Djikeng, A., Feldblyum, T., Field, M. C., Fraser, A., Goodhead, I., Hance, Z., Harper, D., Harris, B. R., Hauser, H., Hostetter, J., Ivens, A., Jagels, K., Johnson, D., Johnson, J., Jones, K., Kerhornou, A. X., Koo, H., Larke, N., Landfear, S., Larkin, C., Leech, V., Line, A., Lord, A., MacLeod, A., Mooney, P. J., Moule, S., Martin, D. M. A., Morgan, G. W., Mungall, K., Norbertczak, H., Ormond, D., Pai, G., Peacock, C. S., Peterson, J., Quail, M. A., Rabbinowitsch, E., Rajandream, M. A., Reitter, C., Salzberg, S. L., Sanders, M., Schobel, S., Sharp, S., Simmonds, M., Simpson, A. J., Talton, L., Turner, C. M. R., Tait, A., Tivey, A. R., Van Aken, S., Walker, D., Wanless, D., Wang, S. L., White, B., White, O., Whitehead, S., Woodward, J., Wortman, J., Adams, M. D., Embley, T. M., Gull, K., Ullu, E., Barry, J. D., Fairlamb, A. H., Opperdoes, F., Barret, B. G., Donelson, J. E., Hall, N., Fraser, C. M., Melville, S. E., and El-Sayed, N. M. (2005) The genome of the African trypanosome Trypanosoma brucei. Science 309, 416-422.
12. Rout, M. P., and Field, M. C. (2001) Isolation and characterization of subnuclear compartments from Trypanosoma brucei-Identification of a major repetitive nuclear lamina component. J. Biol. Chem. 276, 38261-38271.
13. Dreger, M., Bengtsson, L., Schoneberg, T., Otto, H., and Hucho, F. (2001) Nuclear envelope proteomics: Novel integral membrane proteins of the inner nuclear membrane. Proc. Natl. Acad. Sci. USA 98, 11943-11948.
14. Dreger, M. (2003) Proteome analysis at the level of subcellular structures. Eur. J. Biochem. 270, 589-599.
15. Tackett, A. J., Dilworth, D. J., Davey, M. J., O'Donnell, M., Aitchison, J. D., Rout, M. P., and Chait, B. T. (2005) Proteomic and genomic characterization of chromatin complexes at a boundary. J. Cell Biol. 169, 35-47.
16. Archambault, V., Li, C. H. X., Tackett, A. J., Wasch, R., Chait, B. T., Rout, M. P., and Cross, F. R. (2003) Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit. Mol. Biol. Cell 14, 4592-4604.