Artificial sweetener saccharin disrupts intestinal epithelial cells' barrier function in vitro

Food and Function

Volumn 9, Issue 7, 2018, Pages 3815-3822

  Santos, P S ^a   Caria, C R P ^a   Gotardo, E M F ^a   Ribeiro, M L ^a   Pedrazzoli, J ^a   Gambero, A ^a  

^a Universidade Sao Francisco Centro de Ciencias Biologicas e da Saude   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CYTOLOGY; MONOLAYERS; PEPTIDES; SUGARS;

ARTIFICIAL SWEETENERS; BIOLOGICAL INTERACTIONS; EQUIMOLAR CONCENTRATIONS; INTESTINAL EPITHELIAL CELLS; INTESTINAL PERMEABILITIES; NUCLEAR FACTOR KAPPAB; PARACELLULAR PERMEABILITY; TRANSEPITHELIAL ELECTRICAL RESISTANCES;

SUGAR SUBSTITUTES;

CLAUDIN 1; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; SACCHARIN; SWEETENING AGENT;

CACO-2 CELL LINE; DRUG EFFECT; EPITHELIUM CELL; GENETICS; HUMAN; INTESTINE MUCOSA; METABOLISM; PERMEABILITY; TIGHT JUNCTION;

CACO-2 CELLS; CLAUDIN-1; EPITHELIAL CELLS; HUMANS; INTESTINAL MUCOSA; NF-KAPPA B; PERMEABILITY; SACCHARIN; SWEETENING AGENTS; TIGHT JUNCTIONS;

EID: 85050540349     PISSN: 20426496     EISSN: 2042650X     Source Type: Journal    
DOI: 10.1039/c8fo00883c     Document Type: Article

References (38)

1. M. B. Azad A. M. Abou-Setta B. F. Chauhan R. Rabbani J. Lys L. Copstein A. Mann M. M. Jeyaraman A. E. Reid M. Fiander D. S. MacKay J. McGavock B. Wicklow R. Zarychanski Nonnutritive sweeteners and cardiometabolic health: a systematic review and meta-analysis of randomized controlled trials and prospective cohort studies Can. Med. Assoc. J. 2017 189 E929-E939
2. S. S. Gul A. R. Hamilton A. R. Munoz T. Phupitakphol W. Liu S. K. Hyoju K. P. Economopoulos S. Morrison D. Hu W. Zhang M. H. Gharedaghi H. Huo S. R. Hamarneh R. A. Hodin Inhibition of the gut enzyme intestinal alkaline phosphatase may explain how aspartame promotes glucose intolerance and obesity in mice Appl. Physiol., Nutr., Metab. 2017 42 77-83
3. W. N. Cong R. Wang H. Cai C. M. Daimon M. Scheibye-Knudsen V. A. Bohr R. Turkin W. H. Wood, 3rd K. G. Becker R. Moaddel S. Maudsley B. Martin Long-term artificial sweetener acesulfame potassium treatment alters neurometabolic functions in C57BL/6J mice PLoS One 2013 8 e70257
4. J. Suez T. Korem D. Zeevi G. Zilberman-Schapira C. A. Thaiss O. Maza D. Israeli N. Zmora S. Gilad A. Weinberger Y. Kuperman A. Harmelin I. Kolodkin-Gal H. Shapiro Z. Halpern E. Segal E. Elinav Artificial sweeteners induce glucose intolerance by altering the gut microbiota Nature 2014 514 181-186
5. A. C. Meyer-Gerspach B. Wolnerhanssen C. Beglinger Functional roles of low calorie sweeteners on gut function Physiol. Behav. 2016 164 479-481
6. M. Y. Pepino Metabolic effects of non-nutritive sweeteners Physiol. Behav. 2015 152 450-455
7. C. Belloir F. Neiers L. Briand Sweeteners and sweetness enhancers Curr. Opin. Clin. Nutr. Metabol. Care 2017 20 279-285
8. A. N. d. V. S. ANVISA, Regulamento Técnico que autoriza o uso de aditivos edulcorantes em alimentos, com seus respectivos limites máximos, 2008
9. W. A. Colburn I. Bekersky H. P. Blumenthal Dietary saccharin kinetics Clin. Pharmacol. Ther. 1981 30 558-563
10. A. Roberts A. G. Renwick J. Sims D. J. Snodin Sucralose metabolism and pharmacokinetics in man Food Chem. Toxicol. 2000 38 S31-S41
11. S. Puthrasingam W. M. Heybroek A. Johnston V. Maskrey C. G. Swift P. Turner S. M. Abrams S. H. Jackson Aspartame pharmacokinetics-the effect of ageing Age Ageing 1996 25 217-220
12. B. A. Magnuson M. C. Carakostas N. H. Moore S. P. Poulos A. G. Renwick Biological fate of low-calorie sweeteners Nutr. Rev. 2016 74 670-689
13. M. B. Abou-Donia E. M. El-Masry A. A. Abdel-Rahman R. E. McLendon S. S. Schiffman Splenda alters gut microflora and increases intestinal p-glycoprotein and cytochrome p-450 in male rats J. Toxicol. Environ. Health 2008 71 1415-1429
14. R. L. Anderson Effect of saccharin ingestion on stool composition in relation to caecal enlargement and increased stool hydration Food Chem. Toxicol. 1983 21 255-257
15. U. Etxeberria N. Arias N. Boque M. T. Macarulla M. P. Portillo J. A. Martinez F. I. Milagro Reshaping faecal gut microbiota composition by the intake of trans-resveratrol and quercetin in high-fat sucrose diet-fed rats J. Nutr. Biochem. 2015 26 651-660
16. J. E. Nettleton R. A. Reimer J. Shearer Reshaping the gut microbiota: Impact of low calorie sweeteners and the link to insulin resistance? Physiol. Behav. 2016 164 488-493
17. J. Konig J. Wells P. D. Cani C. L. Garcia-Rodenas T. MacDonald A. Mercenier J. Whyte F. Troost R. J. Brummer Human Intestinal Barrier Function in Health and Disease Clin. Transl. Gastroenterol. 2016 7 e196
18. P. D. Cani R. Bibiloni C. Knauf A. Waget A. M. Neyrinck N. M. Delzenne R. Burcelin Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice Diabetes 2008 57 1470-1481
19. B. Chassaing O. Koren J. K. Goodrich A. C. Poole S. Srinivasan R. E. Ley A. T. Gewirtz Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome Nature 2015 519 92-96
20. Y. Zheng M. G. Sarr Effect of the artificial sweetener, acesulfame potassium, a sweet taste receptor agonist, on glucose uptake in small intestinal cell lines J. Gastrointest. Surg. 2013 17 153-158
21. J. Kowapradit P. Opanasopit T. Ngawhirunpat A. Apirakaramwong T. Rojanarata U. Ruktanonchai W. Sajomsang In vitro permeability enhancement in intestinal epithelial cells (Caco-2) monolayer of water soluble quaternary ammonium chitosan derivatives AAPS PharmSciTech 2010 11 497-508
22. Y. Y. Lam C. W. Ha J. M. Hoffmann J. Oscarsson A. Dinudom T. J. Mather D. I. Cook N. H. Hunt I. D. Caterson A. J. Holmes L. H. Storlien Effects of dietary fat profile on gut permeability and microbiota and their relationships with metabolic changes in mice Obesity 2015 23 1429-1439
23. V. Volynets S. Louis D. Pretz L. Lang M. J. Ostaff J. Wehkamp S. C. Bischoff Intestinal Barrier Function and the Gut Microbiome Are Differentially Affected in Mice Fed a Western-Style Diet or Drinking Water Supplemented with Fructose J. Nutr. 2017 147 770-780
24. Q. Yu Z. Wang P. Li Q. Yang The effect of various absorption enhancers on tight junction in the human intestinal Caco-2 cell line Drug Dev. Ind. Pharm. 2013 39 587-592
25. K. Beerens K. De Winter D. Van de Walle C. Grootaert S. Kamiloglu L. Miclotte T. Van de Wiele J. Van Camp K. Dewettinck T. Desmet Biocatalytic Synthesis of the Rare Sugar Kojibiose: Process Scale-Up and Application Testing J. Agric. Food Chem. 2017 65 6030-6041
26. Y. Wang J. B. Mumm R. Herbst R. Kolbeck Y. Wang IL-22 Increases Permeability of Intestinal Epithelial Tight Junctions by Enhancing Claudin-2 Expression J. Immunol. 2017 199 3316-3325
27. V. Garcia-Hernandez M. Quiros A. Nusrat Intestinal epithelial claudins: expression and regulation in homeostasis and inflammation Ann. N. Y. Acad. Sci. 2017 1397 66-79
28. A. G. Markov A. Veshnyakova M. Fromm M. Amasheh S. Amasheh Segmental expression of claudin proteins correlates with tight junction barrier properties in rat intestine J. Comp. Physiol., B 2010 180 591-598
29. M. Furuse M. Hata K. Furuse Y. Yoshida A. Haratake Y. Sugitani T. Noda A. Kubo S. Tsukita Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice J. Cell Biol. 2002 156 1099-1111
30. N. Li P. Lewis D. Samuelson K. Liboni J. Neu Glutamine regulates Caco-2 cell tight junction proteins Am. J. Physiol.: Gastrointest. Liver Physiol. 2004 287 G726-G733
31. D. B. Price M. L. Ackland W. Burks M. I. Knight C. Suphioglu Peanut allergens alter intestinal barrier permeability and tight junction localisation in Caco-2 cell cultures Cell. Physiol. Biochem. 2014 33 1758-1777
32. W. Y. Chen M. Wang J. Zhang S. S. Barve C. J. McClain S. Joshi-Barve Acrolein disrupts tight junction proteins and causes ER stress-mediated epithelial cell death leading to intestinal barrier dysfunction and permeability Am. J. Pathol. 2017 187 2689-2697
33. M. Cao P. Wang C. Sun W. He F. Wang Amelioration of IFN-gamma and TNF-alpha-induced intestinal epithelial barrier dysfunction by berberine via suppression of MLCK-MLC phosphorylation signaling pathway PLoS One 2013 8 e61944
34. A. Fischer M. Gluth U. F. Pape B. Wiedenmann F. Theuring D. C. Baumgart Adalimumab prevents barrier dysfunction and antagonizes distinct effects of TNF-alpha on tight junction proteins and signaling pathways in intestinal epithelial cells Am. J. Physiol.: Gastrointest. Liver Physiol. 2013 304 G970-G979
35. Q. Lei F. Qiang D. Chao W. Di Z. Guoqian Y. Bo Y. Lina Amelioration of hypoxia and LPS-induced intestinal epithelial barrier dysfunction by emodin through the suppression of the NF-kappaB and HIF-1alpha signaling pathways Int. J. Mol. Med. 2014 34 1629-1639
36. D. Gunzel A. S. Yu Claudins and the modulation of tight junction permeability Physiol. Rev. 2013 93 525-569
37. S. Takahashi N. Iwamoto H. Sasaki M. Ohashi Y. Oda S. Tsukita M. Furuse The E3 ubiquitin ligase LNX1p80 promotes the removal of claudins from tight junctions in MDCK cells J. Cell Sci. 2009 122 985-994
38. E. S. Fischer K. Bohm J. R. Lydeard H. Yang M. B. Stadler S. Cavadini J. Nagel F. Serluca V. Acker G. M. Lingaraju R. B. Tichkule M. Schebesta W. C. Forrester M. Schirle U. Hassiepen J. Ottl M. Hild R. E. Beckwith J. W. Harper J. L. Jenkins N. H. Thoma Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide Nature 2014 512 49-53