The rapid emergence of tigecycline resistance in blaKPC-2 harboring Klebsiella pneumoniae, as mediated in vivo by mutation in tetA during tigecycline treatment

Frontiers in Microbiology

Volumn 9, Issue APR, 2018, Pages

  Du, Xiaoxing ^a   He, Fang ^b   Shi, Qiucheng ^a   Zhao, Feng ^a   Xu, Juan ^c   Fu, Ying ^a   Yu, Yunsong ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b PEOPLE S HOSPITAL OF HANGZHOU MEDICAL COLLEGE   (China)

^c INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

Carbapenem resistant Klebsiella pneumoniae; Efflux pump; KPC 2; TetA; Tigecycline resistance

Indexed keywords

CEFOTAXIME; COLISTIN; GENOMIC DNA; IMIPENEM; LEVOFLOXACIN; TIGECYCLINE;

ADULT; AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ANTIBIOTIC THERAPY; ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BACTERIUM ISOLATE; BLA CTX M 65 GENE; BLA KPC 2 GENE; BLA SHV 2 GENE; BLA TEM 1B GENE; CANCER SURGERY; CARBAPENEM-RESISTANT ENTEROBACTERIACEAE; CASE REPORT; CATA2 GENE; CLINICAL ARTICLE; DFRA14 GENE; DUODENUM CARCINOMA; ESCHERICHIA COLI; FEMALE; FOSA GENE; FOSA3 GENE; GENE IDENTIFICATION; HUMAN; IN VIVO STUDY; INDEL MUTATION; KLEBSIELLA PNEUMONIAE; KLEBSIELLA PNEUMONIAE INFECTION; LNU(A) GENE; LOADING DRUG DOSE; MIDDLE AGED; NONHUMAN; PANCREATICODUODENECTOMY; PAPILLARY CARCINOMA; POLYMERASE CHAIN REACTION; QNRS1 GENE; RMTB GENE; SINGLE NUCLEOTIDE POLYMORPHISM; SOUTHERN BLOTTING; SUL2 GENE; TETA GENE; TREATMENT FAILURE; WHOLE GENOME SEQUENCING;

EID: 85045028346     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2018.00648     Document Type: Article

References (27)

1. Akiyama, T., Presedo, J., and Khan, A. A. (2013). The tetA gene decreases tigecycline sensitivity of Salmonella enterica isolates. Int. J. Antimicrob. Agents 42, 133-140. doi: 10.1016/j.ijantimicag.2013.04.017
2. Alhashem, F., Tiren-Verbeet, N. L., Alp, E., and Doganay, M. (2017). Treatment of sepsis: What is the antibiotic choice in bacteremia due to carbapenem resistant Enterobacteriaceae? World J. Clin. Cases 5, 324-332. doi: 10.12998/wjcc.v5.i8.324
3. Barton, B. M., Harding, G. P., and Zuccarelli, A. J. (1995). A general method for detecting and sizing large plasmids. Anal. Biochem. 226, 235-240. doi: 10.1006/abio.1995.1220
4. Beabout, K., Hammerstrom, T. G., Perez, A. M., Magalhaes, B. F., Prater, A. G., Clements, T. P., et al. (2015). The ribosomal S10 protein is a general target for decreased tigecycline susceptibility. Antimicrob. Agents Chemother. 59, 5561-5566. doi: 10.1128/AAC.00547-15
5. Bratu, S., Landman, D., George, A., Salvani, J., and Quale, J. (2009). Correlation of the expression of acrB and the regulatory genes marA, soxS and ramA with antimicrobial resistance in clinical isolates of Klebsiella pneumoniae endemic to New York City. J. Antimicrob. Chemother. 64, 278-283. doi: 10.1093/jac/dkp186
6. Cannatelli, A., Giani, T., D'andrea, M. M., Di Pilato, V., Arena, F., Conte, V., et al. (2014). MgrB inactivation is a common mechanism of colistin resistance in KPC-producing Klebsiella pneumoniae of clinical origin. Antimicrob. Agents Chemother. 58, 5696-5703. doi: 10.1128/AAC.03110-14
7. Chiu, S. K., Huang, L. Y., Chen, H., Tsai, Y. K., Liou, C. H., Lin, J. C., et al. (2017). Roles of ramR and tet(A) mutations in conferring tigecycline resistance in carbapenem-resistant Klebsiella pneumoniae clinical isolates. Antimicrob. Agents Chemother. 61:e00391-17. doi: 10.1128/AAC.00391-17
8. da Silva, R. M., Traebert, J., and Galato, D. (2012). Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae: a review of epidemiological and clinical aspects. Expert Opin. Biol. Ther. 12, 663-671. doi: 10.1517/14712598.2012.681369
9. Fu, Y., Du, X., Ji, J., Chen, Y., Jiang, Y., and Yu, Y. (2012). Epidemiological characteristics and genetic structure of blaNDM-1 in non-baumannii Acinetobacter spp. in China. J. Antimicrob. Chemother. 67, 2114-2122. doi: 10.1093/jac/dks192
10. He, F., Fu, Y., Chen, Q., Ruan, Z., Hua, X., Zhou, H., et al. (2015). Tigecycline susceptibility and the role of efflux pumps in tigecycline resistance in KPC-producing Klebsiella pneumoniae. PLoS One 10:e0119064. doi: 10.1371/journal.pone.0119064
11. Hoban, D. J., Bouchillon, S. K., Johnson, B. M., Johnson, J. L., and Dowzicky, M. J. (2005). In vitro activity of tigecycline against 6792 Gram-negative and Gram-positive clinical isolates from the global Tigecycline Evaluation and Surveillance Trial (TEST Program, 2004). Diagn. Microbiol. Infect. Dis. 52, 215-227. doi: 10.1016/j.diagmicrobio.2005.06.001
12. Iovleva, A., and Doi, Y. (2017). Carbapenem-resistant Enterobacteriaceae. Clin. Lab. Med. 37, 303-315. doi: 10.1016/j.cll.2017.01.005
13. Jenner, L., Starosta, A. L., Terry, D. S., Mikolajka, A., Filonava, L., Yusupov, M., et al. (2013). Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis. Proc. Natl. Acad. Sci. U.S.A. 110, 3812-3816. doi: 10.1073/pnas.1216691110
14. Linkevicius, M., Sandegren, L., and Andersson, D. I. (2016). Potential of tetracycline resistance proteins to evolve tigecycline resistance. Antimicrob. Agents Chemother. 60, 789-796. doi: 10.1128/AAC.02465-15
15. Nielsen, L. E., Snesrud, E. C., Onmus-Leone, F., Kwak, Y. I., Aviles, R., Steele, E. D., et al. (2014). IS5 element integration, a novel mechanism for rapid in vivo emergence of tigecycline nonsusceptibility in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 58, 6151-6156. doi: 10.1128/AAC.03053-14
16. Osei Sekyere, J., Govinden, U., Bester, L. A., and Essack, S. Y. (2016). Colistin and tigecycline resistance in carbapenemase-producing Gram-negative bacteria: emerging resistance mechanisms and detection methods. J. Appl. Microbiol. 121, 601-617. doi: 10.1111/jam.13169
17. Pankey, G. A. (2005). Tigecycline. J. Antimicrob. Chemother. 56, 470-480. doi: 10.1093/jac/dki248
18. Pitout, J. D., Nordmann, P., and Poirel, L. (2015). Carbapenemase-producing Klebsiella pneumoniae, a key pathogen set for global nosocomial dominance. Antimicrob. Agents Chemother. 59, 5873-5884. doi: 10.1128/AAC.01019-15
19. Roy, S., Datta, S., Viswanathan, R., Singh, A. K., and Basu, S. (2013). Tigecycline susceptibility in Klebsiella pneumoniae and Escherichia coli causing neonatal septicaemia (2007-10) and role of an efflux pump in tigecycline non-susceptibility. J. Antimicrob. Chemother. 68, 1036-1042. doi: 10.1093/jac/dks535
20. Ruan, Z., and Feng, Y. (2016). BacWGSTdb, a database for genotyping and source tracking bacterial pathogens. Nucleic Acids Res. 44, D682-D687. doi: 10.1093/nar/gkv1004
21. Ruzin, A., Immermann, F. W., and Bradford, P. A. (2008). Real-time PCR and statistical analyses of acrAB and ramA expression in clinical isolates of Klebsiella pneumoniae. Antimicrob. Agents Chemother. 52, 3430-3432. doi: 10.1128/AAC.00591-08
22. Ruzin, A., Visalli, M. A., Keeney, D., and Bradford, P. A. (2005). Influence of transcriptional activator RamA on expression of multidrug efflux pump AcrAB and tigecycline susceptibility in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 49, 1017-1022. doi: 10.1128/AAC.49.3.1017-1022.2005
23. Veleba, M., Higgins, P. G., Gonzalez, G., Seifert, H., and Schneiders, T. (2012). Characterization of RarA, a novel AraC family multidrug resistance regulator in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 56, 4450-4458. doi: 10.1128/AAC.00456-12
24. Veleba, M., and Schneiders, T. (2012). Tigecycline resistance can occur independently of the ramA gene in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 56, 4466-4467. doi: 10.1128/AAC.06224-11
25. Villa, L., Feudi, C., Fortini, D., Garcia-Fernandez, A., and Carattoli, A. (2014). Genomics of KPC-producing Klebsiella pneumoniae sequence type 512 clone highlights the role of RamR and ribosomal S10 protein mutations in conferring tigecycline resistance. Antimicrob. Agents Chemother. 58, 1707-1712. doi: 10.1128/AAC.01803-13
26. Zankari, E., Hasman, H., Cosentino, S., Vestergaard, M., Rasmussen, S., Lund, O., et al. (2012). Identification of acquired antimicrobial resistance genes. J. Antimicrob. Chemother. 67, 2640-2644. doi: 10.1093/jac/dks261
27. Zhang, R., Liu, L., Zhou, H., Chan, E. W., Li, J., Fang, Y., et al. (2017). Nationwide surveillance of clinical carbapenem-resistant Enterobacteriaceae (CRE) strains in China. EBioMedicine 19, 98-106. doi: 10.1016/j.ebiom.2017.04.032