Directed evolution of the 3-hydroxypropionic acid production pathway by engineering aldehyde dehydrogenase using a synthetic selection device

Metabolic Engineering

Volumn 47, Issue , 2018, Pages 113-120

  Seok, Joo Yeon ^a   Yang, Jina ^b   Choi, Sang Jin ^c   Lim, Hyun Gyu ^a   Choi, Un Jong ^c   Kim, Kyung Jin ^d   Park, Sunghoon ^e   Yoo, Tae Hyeon ^c   Jung, Gyoo Yeol ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b Seoul National University   (South Korea)

^c Ajou University   (South Korea)

^d Kyungpook National University   (South Korea)

^e Ulsan National Institute of Science and Technology   (South Korea)

Author keywords

3 hydroxypropionic acid; Directed evolution; Metabolic engineering; Screening; Synthetic biology; Transcription factor

Indexed keywords

ALDEHYDES; BINDING SITES; ENZYMES; GLYCEROL; METABOLISM; SCREENING; SUBSTRATES; TRANSCRIPTION; TRANSCRIPTION FACTORS;

3-HYDROXYPROPIONIC ACID; ALDEHYDE DEHYDROGENASE; BIOLOGICAL PRODUCTION; CATALYTIC EFFICIENCIES; DIRECTED EVOLUTION; EVOLUTIONARY APPROACH; SEMIALDEHYDE DEHYDROGENASE; SYNTHETIC BIOLOGY;

METABOLIC ENGINEERING;

ALDEHYDE DEHYDROGENASE; ALDEHYDE DERIVATIVE; HYDRACRYLIC ACID; ESCHERICHIA COLI PROTEIN; LACTIC ACID;

ARTICLE; BINDING SITE; CATALYSIS; ENRICHMENT CULTURE; ENZYME ENGINEERING; ENZYME INACTIVATION; ENZYME SUBSTRATE; MOLECULAR EVOLUTION; MOLECULAR LIBRARY; NONHUMAN; PRIORITY JOURNAL; ANALOGS AND DERIVATIVES; BIOSYNTHESIS; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENETICS; METABOLISM;

ALDEHYDE DEHYDROGENASE; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; LACTIC ACID;

EID: 85044109129     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2018.03.009     Document Type: Article

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