Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia

Experimental and Therapeutic Medicine

Volumn 15, Issue 3, 2018, Pages 2884-2890

  Chen, Houyang ^a,b   Luo, Tao ^a   Chen, Tingtao ^a   Wang, Gongxian ^a  

^a NANCHANG UNIVERSITY   (China)

^b JIANGXI PROVINCIAL MATERNAL AND CHILD HEALTH HOSPITAL   (China)

Author keywords

High throughput sequencing; Lactobacillus sp; Microbial diversity; Non obstructive azoospermia; Obstructive azoospermia

Indexed keywords

GENOMIC DNA;

ACTINOBACTERIA; ADULT; ARTICLE; AZOOSPERMIA; BACTEROIDETES; CLINICAL ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; DOWN REGULATION; FIRMICUTES; GENE EXPRESSION; GENE SEQUENCE; HIGH THROUGHPUT SEQUENCING; HUMAN; LACTOBACILLUS; MALE; METABOLISM; MICROBIAL DIVERSITY; NONHUMAN; PHYLOGENY; PLESIOMONAS; PROTEIN EXPRESSION; PROTEOBACTERIA; SEMEN ANALYSIS;

EID: 85041216993     PISSN: 17920981     EISSN: 17921015     Source Type: Journal    
DOI: 10.3892/etm.2018.5778     Document Type: Article

References (32)

1. Luo T, Zou QX, He YQ, Wang HF, Wang T, Liu M, Chen Y and Wang B: Matrine compromises mouse sperm functions by a [Ca(2+)]i-related mechanism. Reprod Toxicol 60: 69-75, 2016.
2. He Y, Zou Q, Li B, Chen H, Du X, Weng S, Luo T and Zeng X: Ketamine inhibits human sperm function by Ca(2+)-related mechanism. Biochem Biophys Res Commun 478: 501-506, 2016.
3. He Y, Zou Q, Chen H, Weng S, Luo T and Zeng X: Lead inhibits human sperm functions by reducing the levels of intracellular calcium, cAMP and tyrosine phosphorylation. Tohoku J Exp Med 238: 295-303, 2016.
4. Hooper LV, Littman DR and Macpherson AJ: Interactions between the microbiota and the immune system. Science 336: 1268-1273, 2012.
5. Nelson MH, Diven MA, Huff LW and Paulos CM: Harnessing the microbiome to enhance cancer immunotherapy. J Immunol Res 2015: 368736, 2015.
6. Nakatsuji T, Chen TH, Narala S, Chun KA, Two AM, Yun T, Shafiq F, Kotol PF, Bouslimani A, Melnik AV, et al: Antimicrobials from human skin commensal bacteria protect against Staphylococcus aureus and are deficient in atopic dermatitis. Sci Transl Med 9: pii: eaah4680, 2017.
7. De Croo I, Van der Elst J, Everaert K, De Sutter P and Dhont M: Fertilization, pregnancy and embryo implantation rates after ICSI in cases of obstructive and non-obstructive azoospermia. Hum Reprod 15: 1383-1388, 2000.
8. Franasiak JM and Scott RT Jr: Reproductive tract microbiome in assisted reproductive technologies. Fertil Steril 104: 1364-1371, 2015.
9. Javurek AB, Spollen WG, Ali AM, Johnson SA, Lubahn DB, Bivens NJ, Bromert KH, Ellersieck MR, Givan SA and Rosenfeld CS: Discovery of a novel seminal fluid microbiome and influence of estrogen receptor alpha genetic status. Sci Rep 6: 23027, 2016.
10. Tuddenham S and Ghanem KG: A microbiome variable in the HIV-prevention equation. Science 356: 907-908, 2017.
11. De Francesco MA, Negrini R, Ravizzola G, Galli P and Manca N: Bacterial species present in the lower male genital tract: A five-year retrospective study. Eur J Contracept Reprod Health Care 16: 47-53, 2011.
12. Younes JA, Lievens E, Hummelen R, van der Westen R, Reid G and Petrova MI: Women and their microbes: The unexpected friendship. Trends Microbiol 26: 16-32, 2018.
13. Weng SL, Chiu CM, Lin FM, Huang WC, Liang C, Yang T, Yang TL, Liu CY, Wu WY, Chang YA, et al: Bacterial communities in semen from men of infertile couples: Metagenomic sequencing reveals relationships of seminal microbiota to semen quality. PLoS One 9: e110152, 2014.
14. Yu X, Wu X, Qiu L, Wang D, Gan M, Chen X, Wei H and Xu F: Analysis of the intestinal microbial community structure of healthy and long-living elderly residents in Gaotian village of liuyang city. Appl Microbiol Biot 99: 9085-9095, 2015.
15. Sivan A, Corrales L, Hubert N, Williams JB, Aquino-Michaels K, Earley ZM, Benyamin FW, Lei YM, Jabri B, Alegre ML, et al: Commensal Bifidobacterium promotes antitumor immunity and facilitates anti-PD-L1 efficacy. Science 350: 1084-1089, 2015.
16. Zhernakova A, Kurilshikov A, Bonder MJ, Tigchelaar EF, Schirmer M, Vatanen T, Mujagic Z, Vila AV, Falony G, Vieira-Silva S, et al: Population-based metagenomics analysis reveals markers for gut microbiome composition and diversity. Science 352: 565-569, 2016.
17. Derrien M and van Hylckama Vlieg JE: Fate, activity and impact of ingested bacteria within the human gut microbiota. Trends Microbiol: 354-366, 2015.
18. Xin F, Xin W, Yang S, Meng F, Wang X, Wei H and Chen T: Evaluation of the microbial diversity in amyotrophic lateral sclerosis using high-throughput sequencing. Front Microbiol 7: 1479, 2016.
19. Cui M, Xiao H, Li Y, Zhou L, Zhao S, Luo D, Zheng Q, Dong J, Zhao Y, Zhang X, et al: Faecal microbiota transplantation protects against radiation-induced toxicity. EMBO Mol Med 4: 448-461, 2017.
20. Chen T, Wu Q, Zhou H, Deng K, Wang X, Meng F, Yang S, Wang X, Shah NP and Wei H: Assessment of commercial probiotic products in China for labelling accuracy and probiotic characterisation of selected isolates. Int J Dairy Technol 70: 119-126, 2017.
21. Xu J, Lian F, Zhao L, Zhao Y, Chen X, Zhang X, Guo Y, Zhang C, Zhou Q, Xue Z, et al: Structural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula. Isme J 9: 552-562, 2015.
22. Bolger A, Lohse M and Usadel B: Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics 30: 2114-2120, 2014.
23. Edgar R: UPARSE: Highly accurate OTU sequences from microbial amplicon reads. Nat Methods 10: 996-998, 2013.
24. Steege HT: Measuring biological diversity. Environ Ecol Stat 1: 95-103, 2004.
25. Cole JR, Wang Q, Fish JA, Chai B, McGarrell DM, Sun Y, Brown CT, Porras-Alfaro A, Kuske CR and Tiedje JM: Ribosomal database project: Data and tools for high throughput rRNA analysis. Nucleic Acids Res 42 (Database Issue): D633-D642, 2014.
26. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, et al: QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7: 335, 2010.
27. Javurek AB, Spollen WG, Johnson SA, Bivens NJ, Bromert KH, Givan SA and Rosenfeld CS: Consumption of a high-fat diet alters the seminal fluid and gut microbiomes in male mice. Reprod Fert Devel 29: 1602-1612, 2017.
28. Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Vega Thurber RL, Knight R, et al: Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol 31: 814-821, 2013.
29. Wolf M, Müller T, Dandekar T and Pollack JD: Phylogeny of Firmicutes with special reference to Mycoplasma (Mollicutes) as inferred from phosphoglycerate kinase amino acid sequence data. Int J Syst Evol Microbiol 54: 871-875, 2004.
30. Donders GG, Bosmans E, Dekeersmaeckerb A, Vereecken A, Van Bulck B and Spitz B: Pathogenesis of abnormal vaginal bacterial flora. Am J Obstet Gyneco 182: 872-878, 2000.
31. Kovachev SM: Obstetric and gynecological diseases and complications resulting from vaginal dysbacteriosis 68: 173-184, 2014.
32. Bae HS, Im WT and Lee ST: Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol 55: 1155-1161, 2005.