A simple, cost-effective method for generating murine colonic 3D enteroids and 2D monolayers for studies of primary epithelial cell function

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 313, Issue 5, 2017, Pages G467-G475

  Fernando, Elizabeth H ^a   Dicay, Michael ^a   Stahl, Martin ^b   Gordon, Marilyn H ^a   Vegso, Andrew ^a   Baggio, Cristiane ^a   Alston, Laurie ^a   Lopes, Fernando ^a   Baker, Kristi ^c   Hirota, Simon ^a   McKay, Derek M ^a   Vallance, Bruce ^b   MacNaughton, Wallace K ^a  

^a UNIVERSITY OF CALGARY   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Epithelial monolayers; Intestine; Mouse; Primary cell culture

Indexed keywords

EOSIN; FLUORESCEIN ISOTHIOCYANATE DEXTRAN; FORSKOLIN; HEMATOXYLIN; MATRIGEL; MUCIN; UVOMORULIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BACTERIAL TRANSLOCATION; CELL CULTURE TECHNIQUE; CELL FUNCTION; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; COLON EPITHELIUM; COLONIC ENTEROID; COST EFFECTIVENESS ANALYSIS; CULTURE OPTIMIZATION; CYTOPLASM; ESCHERICHIA COLI; GOBLET CELL; INTERCELLULAR SPACE; INTESTINE EPITHELIUM CELL; MONOLAYER CULTURE; MOUSE; NONHUMAN; PRIMARY CELL CULTURE; PRIORITY JOURNAL; SHORT CIRCUIT CURRENT; STAINING; THREE DIMENSIONAL IMAGING; TIGHT JUNCTION; TRANSMISSION ELECTRON MICROSCOPY; TWO-DIMENSIONAL IMAGING; ANIMAL; CELL CULTURE; COLON; EPITHELIUM CELL; INTESTINE MUCOSA; ORGANOID; PATHOLOGY; PHYSIOLOGY; PROCEDURES;

ANIMALS; CELLS, CULTURED; COLON; EPITHELIAL CELLS; INTESTINAL MUCOSA; MICE; ORGANOIDS; PRIMARY CELL CULTURE;

EID: 85032951325     PISSN: 01931857     EISSN: 15221547     Source Type: Journal    
DOI: 10.1152/ajpgi.00152.2017     Document Type: Article

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