Heterogeneous genetic location of mcr-1 in colistin-resistant Escherichia coli isolates from humans and retail chicken meat in Switzerland: Emergence of mcr-1-carrying IncK2 plasmids

Antimicrobial Agents and Chemotherapy

Volumn 61, Issue 11, 2017, Pages

  Donà, Valentina ^a   Bernasconi, Odette J ^a   Pires, João ^a   Collaud, Alexandra ^a   Overesch, Gudrun ^a   Ramette, Alban ^a   Perreten, Vincent ^a   Endimiani, Andrea ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

Animals; Chromosome; Chromosome organization; Colistin; E. coli; Escherichia coli; Food; Humans; IncK2; mcr 1; Meat; Plasmids

Indexed keywords

AMPICILLIN; CARBAPENEMASE; CEFOTAXIME; CEFTAZIDIME; CHLORAMPHENICOL; CIPROFLOXACIN; COLISTIN; COTRIMOXAZOLE; DOXYCYCLINE; LEVOFLOXACIN; NALIDIXIC ACID; POLYMYXIN B; SULFAMETHOXAZOLE; TETRACYCLINE; TRIMETHOPRIM; ANTIINFECTIVE AGENT; ESCHERICHIA COLI PROTEIN; MCR-1 PROTEIN, E COLI;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIUM ISOLATE; CHICKEN MEAT; ESCHERICHIA COLI; GENE; GENETIC ANALYSIS; GENETIC IDENTIFICATION; HUMAN; INCHI2 GENE; INCI2 GENE; INCK2 GENE; INCX4 GENE; KLEBSIELLA PNEUMONIAE; MCR 1 GENE; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; PRIORITY JOURNAL; SWITZERLAND; WHOLE GENOME SEQUENCING; ANIMAL; CHICKEN; DRUG EFFECT; ESCHERICHIA COLI INFECTION; FOOD CONTROL; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY;

ANIMALS; ANTI-BACTERIAL AGENTS; CHICKENS; COLISTIN; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FOOD MICROBIOLOGY; HUMANS; PLASMIDS; SWITZERLAND;

EID: 85032490249     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.01245-17     Document Type: Article

References (23)

1. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu LF, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu JH, Shen J. 2016. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 16:161–168. https://doi.org/10.1016/S1473-3099(15)00424-7.
2. Doumith M, Godbole G, Ashton P, Larkin L, Dallman T, Day M, Day M, Muller-Pebody B, Ellington MJ, de Pinna E, Johnson AP, Hopkins KL, Woodford N. 2016. Detection of the plasmid-mediated mcr-1 gene conferring colistin resistance in human and food isolates of Salmonella enterica and Escherichia coli in England and Wales. J Antimicrob Chemother 71:2300–2305. https://doi.org/10.1093/jac/dkw093.
3. Zhao F, Feng Y, Lu X, McNally A, Zong Z. 2017. IncP Plasmid carrying colistin resistance gene mcr-1 in Klebsiella pneumoniae from hospital sewage. Antimicrob Agents Chemother 61:e02229-16. https://doi.org/10 .1128/AAC.02229-16.
4. McGann P, Snesrud E, Maybank R, Corey B, Ong AC, Clifford R, Hinkle M, Whitman T, Lesho E, Schaecher KE. 2016. Escherichia coli harboring mcr-1 and blaCTX-M on a novel IncF plasmid: first report of mcr-1 in the United States. Antimicrob Agents Chemother 60:4420 – 4421. https://doi.org/10.1128/AAC.01103-16.
5. Zhang C, Feng Y, Liu F, Jiang H, Qu Z, Lei M, Wang J, Zhang B, Hu Y, Ding J, Zhu B. 2017. A phage-like incy plasmid carrying the mcr-1 gene in Escherichia coli from a pig farm in China. Antimicrob Agents Chemother 61:e02035– e02016. https://doi.org/10.1128/AAC.02035-16.
6. Zurfluh K, Tasara T, Poirel L, Nordmann P, Stephan R. 2016. Draft genome sequence of Escherichia coli S51, a chicken isolate harboring a chromosomally encoded mcr-1 gene. Genome Announc 4:e00796-16. https:// doi.org/10.1128/genomeA.00796-16.
7. Yu CY, Ang GY, Chong TM, Chin PS, Ngeow YF, Yin WF, Chan KG. 2017. Complete genome sequencing revealed novel genetic contexts of the mcr-1 gene in Escherichia coli strains. J Antimicrob Chemother 72: 1253–1255. https://doi.org/10.1093/jac/dkw541.
8. Sun J, Fang LX, Wu Z, Deng H, Yang RS, Li XP, Li SM, Liao XP, Feng Y, Liu YH. 2017. Genetic analysis of the IncX4 plasmids: implications for a unique pattern in the mcr-1 acquisition. Sci Rep 7:424. https://doi.org/10.1038/s41598-017-00095-x.
9. Bernasconi OJ, Kuenzli E, Pires J, Tinguely R, Carattoli A, Hatz C, Perreten V, Endimiani A. 2016. Travelers can import colistin-resistant Enterobacteriaceae, including those possessing the plasmid-mediated mcr-1 gene. Antimicrob Agents Chemother 60:5080 –5084. https://doi.org/10.1128/AAC.00731-16.
10. Pires J, Bernasconi OJ, Hauser C, Tinguely R, Atkinson A, Perreten V, Dona V, Rauch A, Furrer H, Endimiani A. 2017. Intestinal colonisation with extended-spectrum cephalosporin- and colistin-resistant Enterobacteriaceae in HIV-positive individuals in Switzerland: molecular features and risk factors. Int J Antimicrob Agents 49:519 –521. https://doi.org/10.1016/j.ijantimicag.2017.02.004.
11. Federal Office of Public Health and Federal Food Safety and Veterinary Office. 2016. Swiss antibiotic resistance report 2016. Usage of antibiotics and occurrence of antibiotic resistance in bacteria from humans and animals in Switzerland. FOPH publication number: 2016-OEG-30.
12. Di Pilato V, Arena F, Tascini C, Cannatelli A, Henrici De Angelis L, Fortunato S, Giani T, Menichetti F, Rossolini GM. 2016. mcr-1.2, a new mcr variant carried on a transferable plasmid from a colistin-resistant KPC carbapenemase-producing Klebsiella pneumoniae strain of sequence type 512. Antimicrob Agents Chemother 60:5612–5615. https://doi.org/10.1128/AAC.01075-16.
13. Richter M, Rossello-Mora R. 2009. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 106: 19126–19131. https://doi.org/10.1073/pnas.0906412106.
14. Gascuel O. 1997. BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol 14:685–695. https://doi.org/10.1093/oxfordjournals.molbev.a025808.
15. Seiffert SN, Carattoli A, Schwendener S, Collaud A, Endimiani A, Perreten V. 2017. Plasmids carrying blaCMY-2/4 in Escherichia coli from poultry, poultry meat, and humans belong to a novel IncK subgroup designated IncK2. Front Microbiol 8:407. https://doi.org/10.3389/fmicb.2017.00407.
16. Donà V, Bernasconi OJ, Kasraian S, Tinguely R, Endimiani A. 2017. A SYBR(R) Green-based real-time PCR method for improved detection of mcr-1-mediated colistin resistance in human stool samples. J Glob Antimicrob Resist 9:57–60. https://doi.org/10.1016/j.jgar.2017.01.007.
17. Voets GM, Fluit AC, Scharringa J, Schapendonk C, van den Munckhof T, Leverstein-van Hall MA, Stuart JC. 2013. Identical plasmid AmpC beta-lactamase genes and plasmid types in E. coli isolates from patients and poultry meat in the Netherlands. Int J Food Microbiol 167:359–362. https://doi.org/10.1016/j.ijfoodmicro.2013.10.001.
18. Mo SS, Slettemeas JS, Berg ES, Norstrom M, Sunde M. 2016. Plasmid and host strain characteristics of Escherichia coli resistant to extended-spectrum cephalosporins in the Norwegian broiler production. PLoS One 11:e0154019. https://doi.org/10.1371/journal.pone.0154019.
19. Berg ES, Wester AL, Ahrenfeldt J, Mo SS, Slettemeas JS, Steinbakk M, Samuelsen O, Grude N, Simonsen GS, Lohr IH, Jorgensen SB, Tofteland S, Lund O, Dahle UR, Sunde M. 2017. Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/blaCMY-2 resistance plasmids. Clin Microbiol Infect 23:407.e9 – 407.e15. https://doi.org/10.1016/j.cmi.2016.12.035.
20. European Committee on Antimicrobial Susceptibility Testing. 2016. Breakpoint tables for interpretation of MICs and zone diameters. version 6.0. http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_6.0_Breakpoint_table.pdf.
21. Clinical and Laboratory Standards Institute. 2016. Performance standards for antimicrobial susceptibility testing, 26th ed. CLSI document M100-S26. Clinical and Laboratory Standards Institute, Wayne, PA.
22. Poirel L, Jayol A, Nordmann P. 2017. Polymyxins: antibacterial activity, susceptibility testing, and resistance mechanisms encoded by plasmids or chromosomes. Clin Microbiol Rev 30:557–596. https://doi.org/10.1128/CMR.00064-16.
23. Poirel L, Kieffer N, Nordmann P. 2017. In vitro study of ISApl1-mediated mobilization of the colistin resistance gene mcr-1. Antimicrob Agents Chemother 61:e00127-17. https://doi.org/10.1128/AAC.00127-17.