Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition

Scientific Reports

Volumn 7, Issue 1, 2017, Pages

  Sun, Jian ^a   Fang, Liang Xing ^a   Wu, Zuowei ^b   Deng, Hui ^a   Yang, Run Shi ^a   Li, Xing Ping ^a   Li, Shu Min ^a   Liao, Xiao Ping ^a   Feng, Youjun ^a,c   Liu, Ya Hong ^a  

^a SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^b IOWA STATE UNIVERSITY   (United States)

^c ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT; ESCHERICHIA COLI PROTEIN; MCR-1 PROTEIN, E COLI;

ANTIBIOTIC RESISTANCE; DRUG EFFECT; ESCHERICHIA COLI; GENETICS; HIGH THROUGHPUT SCREENING; PLASMID;

ANTI-BACTERIAL AGENTS; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HIGH-THROUGHPUT SCREENING ASSAYS; PLASMIDS;

EID: 85021678834     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/s41598-017-00095-x     Document Type: Article

References (45)

1. Stokes, H. W. & Gillings, M. R. Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens. Fems Microbiology Reviews 35, 790–819 (2011).
2. Datta, N. & Hughes, V. M. Plasmids of the same Inc groups in Enterobacteria before and after the medical use of antibiotics. Nature 306, 616–617 (1983).
3. Johnson, T. J. et al. Expansion of the IncX plasmid family for improved identification and typing of novel plasmids in drug-resistant Enterobacteriaceae. Plasmid 68, 43–50 (2012).
4. Dobiasova, H. & Dolejska, M. Prevalence and diversity of IncX plasmids carrying fluoroquinolone and beta-lactam resistance genes in Escherichia coli originating from diverse sources and geographical areas. J Antimicrob Chemother 71, 2118–2124 (2016).
5. CTX-M in Escherichia coli from humans and food animals. J Med Microbiol 63, 835–840 (2014).
6. Espedido, B. A. et al. Whole genome sequence analysis of the first Australian OXA-48-producing outbreak-associated Klebsiella pneumoniae isolates: the resistome and in vivo evolution. PLoS One 8, e59920 (2013).
7. Xavier, B. B. et al. Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, In Escherichia coli, Belgium, June 2016. Euro Surveill 21 (2016).
8. CTX-M-14b transposition unit isolated from Escherichia coli and Enterobacter cloacae from cattle. Antimicrob Agents Chemother 57, 4590–4594 (2013).
9. Liu, Y. Y. et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 16, 161–168 (2016).
10. Schwarz, S. & Johnson, A. P. Transferable resistance to colistin: a new but old threat. J Antimicrob Chemother 71, 2066–2070 (2016).
11. Skov, R. L. & Monnet, D. L. Plasmid-mediated colistin resistance (mcr-1 gene): three months later, the story unfolds. Euro Surveill 21 (2016).
12. Ye, H. et al. Diversified mcr-1-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota. mBio 7, e00177 (2016).
13. Li, Z., Tan, C., Lin, J. & Feng, Y. Diversified variants of the mcr-1-carrying plasmid reservoir in the swine lung microbiota. Sci China Life Sci. 1–3 (2016).
14. CTX-M on a Novel IncF Plasmid: First report of mcr-1 in the USA. Antimicrob Agents Chemother (2016).
15. Doumith, M. et al. Detection of the plasmid-mediated mcr-1 gene conferring colistin resistance in human and food isolates of Salmonella enterica and Escherichia coli in England and Wales. J Antimicrob Chemother 71, 2300–2305 (2016).
16. Webb, H. E. et al. Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis 16, 144–145 (2016).
17. Hasman, H. et al. Detection of mcr-1 encoding plasmid-mediated colistin-resistant Escherichia coli isolates from human bloodstream infection and imported chicken meat, Denmark 2015. Euro Surveill 20, 1–5 (2015).
18. Grami, R. et al. Impact of food animal trade on the spread of mcr-1-mediated colistin resistance, Tunisia, July 2015. Euro Surveill 21, 30144 (2016).
19. Zeng, K. J., Doi, Y., Patil, S., Huang, X. & Tian, G. B. Emergence of the Plasmid-Mediated mcr-1 Gene in Colistin-Resistant Enterobacter aerogenes and Enterobacter cloacae. Antimicrob Agents Chemother 60, 3862–3863 (2016).
20. Xavier, B. B., Lammens, C., Butaye, P., Goossens, H. & Malhotra-Kumar, S. Complete sequence of an IncFII plasmid harbouring the colistin resistance gene mcr-1 isolated from Belgian pig farms. J Antimicrob Chemother 71, 2342–2344 (2016).
21. Zurfluh, K., Klumpp, J., Nuesch-Inderbinen, M. & Stephan, R. Full-Length Nucleotide Sequences of mcr-1-Harbouring Plasmids Isolated from Extended-Spectrum-beta-Lactamase-Producing Escherichia coli Isolates of Different Origins. Antimicrob Agents Chemother 60, 5589–5591 (2016).
22. Yu, H. et al. Detection of mcr-1 colistin resistance gene in carbapenem-resistant Enterobacteriaceae (CRE) from different hospitals in China. Antimicrob Agents Chemother, AAC. 00440–00416 (2016).
23. Li, A. et al. Complete Sequences of mcr-1-Harbouring Plasmids from Extended-Spectrum-beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae. Antimicrob Agents Chemother 60, 4351–4354 (2016).
24. Falgenhauer, L. et al. Colistin resistance gene mcr-1 in extended-spectrum beta-lactamase-producing and carbapenemase-producing Gram-negative bacteria in Germany. Lancet Infect Dis 16, 282–283 (2016).
25. Zurfluh, K., Klumpp, J., Nüesch-Inderbinen, M. & Stephan, R. Full-length nucleotide sequences of mcr-1 harbouring plasmids isolated from extended-spectrum β-lactamase (ESBL)-producing Escherichia coli of different origins. Antimicrob Agents Chemother, AAC. 00935–00916 (2016).
26. Veldman, K. et al. Location of colistin resistance gene mcr-1 in Enterobacteriaceae from livestock and meat. J Antimicrob Chemother, dkw181 (2016).
27. Campos, J., Cristino, L., Peixe, L. & Antunes, P. MCR-1 in multidrug-resistant and copper-tolerant clinically relevant Salmonella 1,4,[5],12:i:- and S. Rissen clones in Portugal, 2011 to 2015. Euro Surveill 21 (2016).
28. Fernandes, M. R. et al. First Report of the Globally Disseminated IncX4 Plasmid Carrying the mcr-1 Gene in a Colistin-Resistant Escherichia coli ST101 isolated from a Human Infection in Brazil. Antimicrob Agents Chemother, AAC. 01325–01316 (2016).
29. Poirel, L. et al. Genetic Features of MCR-1-Producing Colistin-Resistant Escherichia coli Isolates in South Africa. Antimicrob Agents Chemother 60, 4394–4397 (2016).
30. CTX-M-55 and mcr-1. Antimicrob Agents Chemother 60, 5014–5017 (2016).
31. Snesrud, E. et al. A Model for Transposition of the Colistin Resistance Gene mcr-1 by ISApl1. Antimicrob Agents Chemother (2016).
32. CTX-M-15 from an Escherichia coli ST131 isolate. Antimicrob Agents Chemother 55, 5933–5935 (2011).
33. Li, R. et al. Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant. J Antimicrob Chemother (2016).
34. Tegetmeyer, H. E., Jones, S. C., Langford, P. R. & Baltes, N. ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76. Vet Microbiol 128, 342–353 (2008).
35. Mahillon, J. & Chandler, M. Insertion sequences. Microbiol Mol Biol Rev 62, 725–774 (1998).
36. Porse, A., Schonning, K., Munck, C. & Sommer, M. O. Survival and Evolution of a Large Multidrug Resistance Plasmid in New Clinical Bacterial Hosts. Mol Biol Evol (2016).
37. Gautom, R. K. Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. J Clin Microbiol 35, 2977–2980 (1997).
38. EUCAST. Breakpoint tables for interpretation of MICs and zone diameters. The European Committee on Antimicrobial Susceptibility Testing (2016).
39. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. CLSI document M100-S25. Clinical and Laboratory Standards Institute Wayne, PA (2015).
40. CLSI. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacterial Isolated from Animals. Approved Standard-Fourth Edition and Supplement. CLSI documents VET01A4E and VET01S3E. Clinical and Laboratory Standards Institute Wayne, PA (2015).
41. Yang, R. S. et al. Emergence of NDM-5 and MCR-1-Producing Escherichia coli Clone ST648 and ST156 from A Single Muscovy Duck (Cairina moschata). Antimicrob Agents Chemother (2016).
42. Carattoli, A. et al. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods 63, 219–228 (2005).
43. Luo, R. et al. SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. GigaScience 1, 18 (2012).
44. Aziz, R. K. et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9, 75 (2008).
45. Sullivan, M. J., Petty, N. K. & Beatson, S. A. Easyfig: a genome comparison visualizer. Bioinformatics 27, 1009–1010 (2011).