Identification of biosynthetic gene clusters from metagenomic libraries using PPTase complementation in a Streptomyces host

FEMS Microbiology Letters

Volumn 364, Issue 16, 2017, Pages

  Bitok, J Kipchirchir ^a   Lemetre, Christophe ^a   Ternei, Melinda A ^a   Brady, Sean F ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

EDNA; Environmental DNA; Functional metagenomics; Myxochelin; PPTase; Streptomyces albus

Indexed keywords

4' PHOSPHOPANTETHEINYL TRANSFERASE; ACYL CARRIER PROTEIN; DNA; ENVIRONMENTAL DNA; INDIGOIDINE; IRON CHELATING AGENT; MYXOCHELIN A; NONRIBOSOMAL PEPTIDE SYNTHETASE; PEPTIDYL CARRIER PROTEIN; PIGMENT; POLYKETIDE SYNTHASE; TRANSFERASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; BIOLOGICAL PRODUCT; PEPTIDE SYNTHASE; PHOSPHOPANTETHEINYL TRANSFERASE; PHOSPHOTRANSFERASE; PIPERIDONE DERIVATIVE;

BIOSYNTHESIS; BIOSYNTHETIC GENE CLUSTER; BPSA GENE; CONTROLLED STUDY; GENE; GENE CLUSTER; GENE EDITING; GENE IDENTIFICATION; GENE LIBRARY; GENETIC COMPLEMENTATION; HETEROLOGOUS EXPRESSION; LETTER; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; METAGENOMICS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENETIC TREE; PRIORITY JOURNAL; SOIL; STREPTOMYCES ALBUS; BACTERIAL GENE; CLASSIFICATION; COSMID; ESCHERICHIA COLI; GENETICS; METABOLISM; METAGENOME; MICROBIOLOGY; MULTIGENE FAMILY; PHYLOGENY; PROCEDURES; STREPTOMYCES;

BACTERIAL PROTEINS; BIOLOGICAL PRODUCTS; CLONING, MOLECULAR; COSMIDS; ESCHERICHIA COLI; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; GENOMIC LIBRARY; METAGENOME; METAGENOMICS; MULTIGENE FAMILY; PEPTIDE SYNTHASES; PHYLOGENY; PIPERIDONES; SOIL MICROBIOLOGY; STREPTOMYCES; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

EID: 85031931508     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1093/femsle/fnx155     Document Type: Letter

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