Optimized retroviral transduction of mouse T cells for in vivo assessment of gene function

Nature Protocols

Volumn 12, Issue 9, 2017, Pages 1980-1998

  Kurachi, Makoto ^a   Kurachi, Junko ^a   Chen, Zeyu ^a   Johnson, John ^a,b   Khan, Omar ^a   Bengsch, Bertram ^a   Stelekati, Erietta ^a   Attanasio, John ^a   McLane, Laura M ^a   Tomura, Michio ^c   Ueha, Satoshi ^d   Wherry, E John ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c OSAKA OHTANI UNIVERSITY   (Japan)

^d UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

REAGENT; RETROVIRUS VECTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CD8+ T LYMPHOCYTE; CELL ISOLATION; CELL VIABILITY; CONTROLLED STUDY; CULTURE OPTIMIZATION; DENSITY GRADIENT CENTRIFUGATION; ENRICHMENT CULTURE; GENE EXPRESSION; GENE FUNCTION; GENE TARGETING; GENETIC TRANSDUCTION; IN VITRO STUDY; IN VIVO STUDY; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; RETROVIRIDAE; T LYMPHOCYTE ACTIVATION; TIME; VIRAL GENE DELIVERY SYSTEM; ANIMAL; GENE VECTOR; GENETICS; PROCEDURES; VIROLOGY;

ANIMALS; CD8-POSITIVE T-LYMPHOCYTES; GENETIC VECTORS; MICE; RETROVIRIDAE; TRANSDUCTION, GENETIC;

EID: 85029668229     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2017.083     Document Type: Article

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