Retroviral transduction of T-cell receptors in mouse T-cells

Journal of Visualized Experiments

Volumn , Issue 44, 2010, Pages

  Zhong, Shi ^a   Malecek, Karolina ^a,b   Perez Garcia, Arianne ^a   Krogsgaard, Michelle ^a  

^a NEW YORK UNIVERSITY MEDICAL CENTER   (United States)

^b NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355143991     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2307     Document Type: Article

References (11)

1. Krogsgaard, M. & Davis, M.M. How T cells 'see' antigen. Nat. Immunol 6, 239-245 (2005).
2. Berg, L.J. et al. Antigen/MHC-specific T cells are preferentially exported from the thymus in the presence of their MHC ligand. Cell 58, 1035-1046 (1989).
3. Hogquist, K.A. et al. T cell receptor antagonist peptides induce positive selection. Cell 76, 17-27 (1994).
4. Yu, Z. et al. Poor immunogenicity of a self/tumor antigen derives from peptide-MHC-I instability and is independent of tolerance. J. Clin. Invest 114, 551-559 (2004).
5. Holst, J. et al. Generation of T-cell receptor retrogenic mice. Nat Protoc 1, 406-417 (2006).
6. Morgan, R.A. et al. Cancer regression in patients after transfer of genetically engineered lymphocytes. Science 314, 126-129 (2006).
7. Hawley, R.G., Lieu, F.H., Fong, A.Z. & Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther 1, 136-138 (1994).
8. Szymczak, A.L. et al. Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat. Biotechnol 22, 589-594 (2004).
9. Cohen, C.J., Zhao, Y., Zheng, Z., Rosenberg, S.A. & Morgan, R.A. Enhanced antitumor activity of murine-human hybrid T-cell receptor (TCR) in human lymphocytes is associated with improved pairing and TCR/CD3 stability. Cancer Res 66, 8878-8886 (2006).
10. Letourneur, F. & Malissen, B. Derivation of a T cell hybridoma variant deprived of functional T cell receptor alpha and beta chain transcripts reveals a nonfunctional alpha-mRNA of BW5147 origin. Eur. J. Immunol 19, 2269-2274 (1989).
11. Bendle, G.M. et al. Lethal graft-versus-host disease in mouse models of T cell receptor gene therapy. Nat. Med 16, 565-570, 1p following 570 (2010).