Metabolic engineering of Escherichia coli to produce 2′-fucosyllactose via salvage pathway of guanosine 5′-diphosphate (GDP)-l-fucose

Biotechnology and Bioengineering

Volumn 113, Issue 11, 2016, Pages 2443-2452

  Chin, Young Wook ^a   Seo, Nari ^b   Kim, Jae Han ^b   Seo, Jin Ho ^a  

^a Seoul National University   (South Korea)

^b Chungnam National University   (South Korea)

Author keywords

2 fucosyllactose; engineered Escherichia coli; fucokinase GDP l fucose pyrophosphorylase; fucose

Indexed keywords

BIOMOLECULES; ESCHERICHIA COLI; FERMENTATION; GENE ENCODING; GENES; PRODUCTIVITY; SUGARS;

BACTEROIDES FRAGILIS; BIOSYNTHETIC PATHWAY; ENGINEERED ESCHERICHIA COLI; EXTRACELLULAR CONCENTRATION; FED-BATCH FERMENTATION; FUCOKINASE/GDP-L-FUCOSE PYROPHOSPHORYLASE; FUCOSE; FUCOSYLLACTOSE;

METABOLIC ENGINEERING;

2' FUCOSYLLACTOSE; BACTERIAL ENZYME; BETA GALACTOSIDASE; FUCOKINASE; FUCOSE; FUCOSE ISOMERASE; FUCOSYLTRANSFERASE; FUCULOSE KINASE; GLYCEROL; GUANOSINE 5' DIPHOSPHATE LEVO FUCOSE; GUANOSINE 5' DIPHOSPHATE LEVO FUCOSE PYROPHOSPHORYLASE; GUANOSINE DIPHOSPHATE FUCOSE; LACTOSE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; BACTERIUM MUTANT; BACTEROIDES FRAGILIS; CARBON SOURCE; CONTROLLED STUDY; DELETION MUTANT; ESCHERICHIA COLI; FED BATCH FERMENTATION; FUCI GENE; FUCK GENE; FUCT2 GENE; GENE CLUSTER; HELICOBACTER PYLORI; LACTOSE OPERON; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METABOLIC ENGINEERING; METABOLIC REGULATION; NONHUMAN; PLASMID; TANDEM MASS SPECTROMETRY;

EID: 85027927081     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26015     Document Type: Article

References (22)

1. Albermann C, Piepersberg W, Wehmeier U. 2001. Synthesis of the milk oligosaccharide 2'-fucosyllactose using recombinant bacterial enzymes. Carbohydr Res 334(2):97–103.
2. Baumgärtner F, Seitz L, Sprenger GA, Albermann C. 2013. Construction of Escherichia coli strains with chromosomally integrated expression cassettes for the synthesis of 2'-fucosyllactose. Microb Cell Fact 12(1):40.
3. Brown MR. 1991. The amino acid and sugar composition of 16 species of microalgae used in mariculture. J Exp Mar Bio Ecol 145(1):79–99.
4. Bulter T, Elling L. 1999. Enzymatic synthesis of nucleotide sugars. Glycoconj J 16(2):147–159.
5. Butler W, Serif GS. 1985. Fucokinase, its anomeric specificity and mechanism of phosphate group transfer. Biochim Biophys Acta 829(2):238–243.
6. Castanys-Muñoz E, Martin MJ, Prieto PA. 2013. 2′-Fucosyllactose: An abundant, genetically determined soluble glycan present in human milk. Nutr Rev 71(12):773–789.
7. Chakrabarti T, Chen Y, Lin E. 1984. Clustering of genes for L-fucose dissimilation by Escherichia coli. J Bacteriol 157(3):984–986.
8. Chin YW, Kim JY, Lee WH, Seo JH. 2015. Enhanced production of 2′-fucosyllactose in engineered Escherichia coli BL21star(DE3) by modulation of lactose metabolism and fucosyltransferase. J Biotechnol 210:107–115.
9. Coffey J, Miller ON, Sellinger OZ. 1964. The metabolism of L-fucose in the rat. J Biol Chem 239(12):4011–4017.
10. Coyne MJ, Reinap B, Lee MM, Comstock LE. 2005. Human symbionts use a host-like pathway for surface fucosylation. Science 307(5716):1778–1781.
11. Datsenko KA, Wanner BL. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97(12):6640.
12. Hüfner E, Parkot J, Jennewein S; WIPO patent 2010070104, assignee. 2011 Dec 8. Synthesis of fucosylated compounds.
13. Han NS, Kim TJ, Park YC, Kim J, Seo JH. 2012. Biotechnological production of human milk oligosaccharides. Biotechnol Adv 30(6):1268–1278.
14. Ishihara H, Heath EC. 1968. The metabolism of L-fucose IV. The biosynthesis of guanosine diphosphate l-fucose in porcine liver. J Biol Chem 243(6):1110–1115.
15. Lee WH, Pathanibul P, Quarterman J, Jo JH, Han NS, Miller MJ, Jin YS, Seo JH. 2012. Whole cell biosynthesis of a functional oligosaccharide, 2'-fucosyllactose, using engineered Escherichia coli. Microb Cell Fact 11(1):48.
16. Liu TW, Ito H, Chiba Y, Kubota T, Sato T, Narimatsu H. 2011. Functional expression of l-fucokinase/guanosine 5′-diphosphate-l-fucose pyrophosphorylase from Bacteroides fragilis in Saccharomyces cerevisiae for the production of nucleotide sugars from exogenous monosaccharides. Glycobiology 21(9):1228–1236.
17. McKenzie GJ, Craig NL. 2006. Fast, easy and efficient: Site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event. BMC Microbiol 6(1):39.
18. Park J, Hong S-K, Chang YK. 2015. Production of DagA and ethanol by sequential utilization of sugars in a mixed-sugar medium simulating microalgal hydrolysate. Bioresour Technol 191:414–419.
19. Park SH, Pastuszak I, Drake R, Elbein AD. 1998. Purification to apparent homogeneity and properties of pig kidney L-fucose kinase. J Biol Chem 273(10):5685–5691.
20. Subedi KP, Kim I, Kim J, Min B, Park C. 2008. Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K-12. FEMS Microbiol Lett 279(2):180–187.
21. Zhai Y, Han D, Pan Y, Wang S, Fang J, Wang P, Liu X-w. 2015. Enhancing GDP-fucose production in recombinant Escherichia coli by metabolic pathway engineering. Enzyme Microb Technol 69:38–45.
22. Zhu Y, Lin E. 1989. L-1, 2-Propanediol exits more rapidly than L-lactaldehyde from Escherichia coli. J Bacteriol 171(2):862–867.