Efficient generation of hypothalamic neurons from human pluripotent stem cells

Current Protocols in Human Genetics

Volumn 2016, Issue , 2016, Pages 21.5.1-21.5.14

  Wang, Liheng ^a   Egli, Dieter ^a,b   Leibel, Rudolph L ^a  

^a Columbia University College of Physicians and Surgeons ^*  (United States)

^b New York Stem Cell Foundation Laboratory   (United States)

Author keywords

ARC neurons; Feeder free; Human pluripotent stem cells; Hypothalamic neurons

Indexed keywords

BONE MORPHOGENETIC PROTEIN; BRAIN DERIVED NEUROTROPHIC FACTOR; NOTCH RECEPTOR; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR NKX2.1; TRANSFORMING GROWTH FACTOR BETA; UNCLASSIFIED DRUG; NEUROPEPTIDE Y; PROOPIOMELANOCORTIN;

ANIMAL CELL; ANIMAL TISSUE; ARCUATE NUCLEUS; ARTICLE; CELL DENSITY; CELL DIFFERENTIATION; CELL MATURATION; CONTROLLED STUDY; CRYOPRESERVATION; EMBRYO; FIBROBLAST; HUMAN; HUMAN CELL; HYPOTHALAMUS; MOUSE; NERVE CELL; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN SYNTHESIS REGULATION; SHH SIGNALING; SIGNAL TRANSDUCTION; CYTOLOGY; METABOLISM; NEURAL STEM CELL;

ARCUATE NUCLEUS OF HYPOTHALAMUS; CELL DIFFERENTIATION; HUMANS; NEURAL STEM CELLS; NEURONS; NEUROPEPTIDE Y; PLURIPOTENT STEM CELLS; PRO-OPIOMELANOCORTIN; SIGNAL TRANSDUCTION;

EID: 85026495708     PISSN: 19348266     EISSN: 19348258     Source Type: Journal    
DOI: 10.1002/cphg.3     Document Type: Article

References (20)

1. Albuquerque, C., Joseph, D.J., Choudhury, P., and MacDermott, A.B. 2009. Dissection, plating, and maintenance of cortical astrocyte cultures. Cold Spring Harb. Protoc. 2009: pdb.prot5273.
2. Aujla, P.K., Naratadam, G.T., Xu, L., and Raetzman, L.T. 2013. Notch/Rbpjkappa signaling regulates progenitor maintenance and differentiation of hypothalamic arcuate neurons. Development 140: 3511-3521. doi: 10.1242/dev.098681.
3. Barsh, G.S. and Schwartz, M.W. 2002. Genetic approaches to studying energy balance: perception and integration. Nat. Rev. Genet. 3: 589-600.
4. Bock, C., Kiskinis, E., Verstappen, G., Gu, H., Boulting, G., Smith, Z.D., Ziller, M., Croft, G.F., Amoroso, M.W., Oakley, D.H., Gnirke, A., Eggan, K., and Meissner, A. 2011. Reference Maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines. Cell 144: 439-452. doi: 10.1016/j.cell.2010.12.032.
5. Chambers, S.M., Fasano, C.A., Papapetrou, E.P., Tomishima, M., Sadelain, M., and Studer, L. 2009. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat. Biotechnol. 27: 275-280. doi: 10.1038/nbt.1529.
6. Chambers, S.M., Qi, Y., Mica, Y., Lee, G., Zhang, X.J., Niu, L., Bilsland, J., Cao, L., Stevens, E., Whiting, P., Shi, S.-H., and Studer, L. 2012. Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors. Nat. Biotechnol. 30: 715-720. doi: 10.1038/nbt.2249.
7. Crawford, T.Q. and Roelink, H. 2007. The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling. Dev. Dyn. 236: 886-892. doi: 10.1002/dvdy.21083.
8. Goulburn, A.L., Alden, D., Davis, R.P., Micallef, S.J., Ng, E.S., Yu, Q.C., Lim, S.M., Soh, C.L., Elliott, D.A., Hatzistavrou, T., Bourke, J., Watmuff, B., Lang, R.J., Haynes, J.M., Pouton, C.W., Giudice, A., Trounson, A.O., Anderson, S.A., Stanley, E.G., and Elefanty, A.G. 2011. A targeted NKX2.1 human embryonic stem cell reporter line enables identification of human basal forebrain derivatives. Stem Cells 29: 462-473. doi: 10.1002/stem.587.
9. Kime, C., Rand, T.A., Ivey, K.N., Srivastava, D., Yamanaka, S., and Tomoda, K. 2015. Practical integration-free episomal methods for generating human induced pluripotent stem cells. Curr. Protoc. Hum. Genet. 87: 21 22 21-21 22 21. doi: 10.1002/0471142905.hg2102s87.
10. Koyanagi-Aoi, M., Ohnuki, M., Takahashi, K., Okita, K., Noma, H., Sawamura, Y., Teramoto, I., Narita, M., Sato, Y., Ichisaka, T., Amano, N., Watanabe, A., Morizane, A., Yamada, Y., Sato, T., Takahashi, J., and Yamanaka, S. 2013. Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells. Proc. Natl. Acad. Sci. U. S. A. 110: 20569-20574. doi: 10.1073/pnas.1319061110.
11. Liu, Y.,Weick, J.P., Liu, H., Krencik, R., Zhang, X., Ma, L., Zhou, G.M., Ayala,M., and Zhang, S.C. 2013. Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits. Nat. Biotechnol. 31: 440-447. doi: 10.1038/nbt.2565.
12. Mallon, B.S., Park, K.Y., Chen, K.G., Hamilton, R.S., and McKay, R.D. 2006. Toward xenofree culture of human embryonic stem cells. Int. J. Biochem. Cell Biol. 38: 1063-1075. doi: 10.1016/j.biocel.2005.12.014.
13. Marcelle, C., Stark, M.R., and Bronner-Fraser, M. 1997. Coordinate actions of BMPs, Wnts, Shh and noggin mediate patterning of the dorsal somite. Development 124: 3955-3963.
14. Maroof, A.M., Keros, S., Tyson, J.A., Ying, S.W., Ganat, Y.M., Merkle, F.T., Liu, B., Goulburn, A., Stanley, E.G., Elefanty, A.G., Widmer, H.R., Eggan, K., Goldstein, P.A., Anderson, S.A., and Studer, L. 2013. Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells. Cell Stem Cell 12: 559-572. doi: 10.1016/j.stem.2013.04.008.
15. Merkle, F.T., Maroof, A., Wataya, T., Sasai, Y., Studer, L., Eggan, K., and Schier, A.F. 2015. Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells. Development 142: 633-643. doi: 10.1242/dev.117978.
16. Nelson, B.R., Hartman, B.H., Georgi, S.A., Lan, M.S., and Reh, T.A. 2007. Transient inactivation of Notch signaling synchronizes differentiation of neural progenitor cells. Dev. Biol. 304: 479-498. doi: 10.1016/j.ydbio.2007.01.001.
17. Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S., and Jones, J.M. 1998. Embryonic stem cell lines derived from human blastocysts. Science 282: 1145-1147. doi: 10.1126/ science.282.5391.1145.
18. van der Klaauw, A.A. and Farooqi, I.S. 2015. The hunger genes: pathways to obesity. Cell 161: 119-132. doi: 10.1016/j.cell.2015.03.008.
19. Wang, L., Meece, K., Williams, D.J., Lo, K.A., Zimmer, M., Heinrich, G., Martin Carli, J., Leduc, C.A., Sun, L., Zeltser, L.M., Freeby, M., Goland, R., Tsang, S.H., Wardlaw, S.L., Egli, D., and Leibel, R.L. 2015. Differentiation of hypothalamic-like neurons from human pluripotent stem cells. J. Clin. Invest. 125: 796-808. doi: 10.1172/JCI79220.
20. Wataya, T., Ando, S., Muguruma, K., Ikeda, H., Watanabe, K., Eiraku, M., Kawada, M., Takahashi, J., Hashimoto, N., and Sasai, Y. 2008. Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation. Proc. Natl. Acad. Sci. U.S.A. 105: 11796-11801.