Genetic interaction mapping in mammalian cells using CRISPR interference

Nature Methods

Volumn 14, Issue 6, 2017, Pages 577-580

  Du, Dan ^a,b   Roguev, Assen ^c,d   Gordon, David E ^c,d   Chen, Meng ^a,e   Chen, Si Han ^c,d   Shales, Michael ^c,d   Shen, John Paul ^f,g   Ideker, Trey ^f,g   Mali, Prashant ^g   Qi, Lei S ^a,b   Krogan, Nevan J ^c,d,e  

^a Stanford University   (United States)

^b STANFORD UNIVERSITY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e GLADSTONE INSTITUTES   (United States)

^f UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^g UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRISPR ASSOCIATED PROTEIN; GUIDE RNA;

ARTICLE; CHROMATIN; CHROMATIN STRUCTURE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENE INTERACTION; GENE MAPPING; HUMAN; HUMAN CELL; HUMAN GENOME; MAMMAL CELL; NEXT GENERATION SEQUENCING; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; ANIMAL; CHROMOSOMAL MAPPING; EPISTASIS; GENETICS; HIGH THROUGHPUT SEQUENCING; MOUSE; PROCEDURES; PROTEIN ANALYSIS;

ANIMALS; CHROMOSOME MAPPING; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; EPISTASIS, GENETIC; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MICE; PROTEIN INTERACTION MAPPING;

EID: 85021812886     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.4286     Document Type: Article

References (27)

1. Beltrao, P., Cagney, G., Krogan, N.J. Cell 141, 739-745 (2010).
2. Collins, S.R. et al. Nature 446, 806-810 (2007).
3. Kelley, R., Ideker, T. Nat. Biotechnol. 23, 561-566 (2005).
4. Roguev, A. et al. Science 322, 405-410 (2008).
5. Costanzo, M. et al. Science 353, aaf1420 (2016).
6. Fischer, B. et al. eLife 4, e05464 (2015).
7. Horn, T. et al. Nat. Methods 8, 341-346 (2011).
8. Roguev, A. et al. Nat. Methods 10, 432-437 (2013).
9. Laufer, C., Fischer, B., Billmann, M., Huber, W., Boutros, M. Nat. Methods 10, 427-431 (2013).
10. Hannon, G.J. Nature 418, 244-251 (2002).
11. Morgens, D.W., Deans, R.M., Li, A., Bassik, M.C. Nat. Biotechnol. 34, 634-636 (2016).
12. Gilbert, L.A. et al. Cell 154, 442-451 (2013).
13. Hilton, I.B. et al. Nat. Biotechnol. 33, 510-517 (2015).
14. Gilbert, L.A. et al. Cell 159, 647-661 (2014).
15. Konermann, S. et al. Nature 517, 583-588 (2015).
16. Collins, S.R., Schuldiner, M., Krogan, N.J., Weissman, J.S. Genome Biol. 7, R63 (2006).
17. Lee, J. et al. Proc. Natl. Acad. Sci. USA 106, 8513-8518 (2009).
18. Kim, D.-H., Lee, J., Lee, B., Lee, J.W. Mol. Endocrinol. 23, 1556-1562 (2009).
19. Kittler, R., Heninger, A.-K., Franke, K., Habermann, B., Buchholz, F. Nat. Methods 2, 779-784 (2005).
20. Mandegar, M.A. et al. Cell Stem Cell 18, 541-553 (2016).
21. Du, D., Qi, L.S. Cold Spring Harb. Protoc. 2016 http://dx.doi.org/10.1101/pdb.prot090175 (2016).
22. Du, D., Qi, L.S. Cold Spring Harb. Protoc. 2016 https://dx.doi.org/10.1101/pdb.top086835 (2016).
23. Liu, H. et al. Bioinformatics 31, 3676-3678 (2015).
24. Wilmes, G.M. et al. Mol. Cell 32, 735-746 (2008).
25. Fiedler, D. et al. Cell 136, 952-963 (2009).
26. Aguilar, P.S. et al. Nat. Struct. Mol. Biol. 17, 901-908 (2010).
27. Surma, M.A. et al. Mol. Cell 51, 519-530 (2013).