Comparison of different methods for telomere length measurement in whole blood and blood cell subsets: Recommendations for telomere length measurement in hematological diseases

Genes Chromosomes and Cancer

Volumn 56, Issue 9, 2017, Pages 700-708

  Behrens, Yvonne Lisa ^a   Thomay, Kathrin ^a   Hagedorn, Maike ^a   Ebersold, Juliane ^a   Henrich, Lea ^a   Nustede, Rainer ^a   Schlegelberger, Brigitte ^a   Göhring, Gudrun ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; AGE; AGED; ANALYTIC METHOD; ARTICLE; BLOOD CELL; CELL SUBPOPULATION; CENTROMERE; CHILD; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GOLD STANDARD; HEMATOLOGIC DISEASE; HUMAN; HUMAN CELL; INFANT; INTERMETHOD COMPARISON; LEUKOCYTE; MALE; MEASUREMENT ACCURACY; NEWBORN; NORMAL HUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; RESTRICTION FRAGMENT; SOUTHERN BLOTTING; TELOMERE; TELOMERE LENGTH; TELOMERE SHORTENING; UMBILICAL CORD BLOOD; BLOOD; COMPARATIVE STUDY; GENETIC SCREENING; GENETICS; MIDDLE AGED; PRESCHOOL CHILD; PROCEDURES; STANDARDS; VERY ELDERLY;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BLOTTING, SOUTHERN; CHILD; CHILD, PRESCHOOL; FEMALE; GENETIC TESTING; HEMATOLOGIC DISEASES; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INFANT; INFANT, NEWBORN; MALE; MIDDLE AGED; TELOMERE SHORTENING;

EID: 85021753782     PISSN: 10452257     EISSN: 10982264     Source Type: Journal    
DOI: 10.1002/gcc.22475     Document Type: Article

References (33)

1. Aubert G, Hills M, Lansdorp PM. Telomere length measurement-caveats and a critical assessment of the available technologies and tools. Mutat Res. 2012;730:59–67.
2. Flores I, Canela A, Vera E, Tejera A, Cotsarelis G, Blasco MA. The longest telomeres: a general signature of adult stem cell compartments. Genes Dev. 2008;22:654–667.
3. Martinez P, Blasco MA. Telomeric and extra-telomeric roles for telomerase and the telomere-binding proteins. Nat Rev Cancer 2011;11:161–176.
4. Milyavsky M, Mimran A, Senderovich S, et al. Activation of p53 protein by telomeric (TTAGGG)n repeats. Nucleic Acids Res. 2001;29:5207–5215.
5. Hiyama E, Hiyama K. Telomere and telomerase in stem cells. Br J Cancer 2007;96:1020–1024.
6. Collins K, Mitchell JR. Telomerase in the human organism. Oncogene 2002;21:564–579.
7. Holohan B, Wright WE, Shay JW. Cell biology of disease: telomeropathies: an emerging spectrum disorder. J Cell Biol. 2014;205:289–299.
8. Gramatges MM, Bertuch AA. Short telomeres: from dyskeratosis congenita to sporadic aplastic anemia and malignancy. Transl Res. 2013;162:353–363.
9. Alter BP, Giri N, Savage SA, Rosenberg PS. Cancer in dyskeratosis congenita. Blood 2009;113:6549–6557.
10. Young NS. Bone marrow failure and the new telomere diseases: practice and research. Hematology 2012;17:S18–S21.
11. Montpetit AJ, Alhareeri AA, Montpetit M, et al. Telomere length: a review of methods for measurement. Nurs Res. 2014;63:289–299.
12. Southern EM. Measurement of DNA length by gel electrophoresis. Anal Biochem. 1979;100:319–323.
13. Gohring J, Fulcher N, Jacak J, Riha K. TeloTool: a new tool for telomere length measurement from terminal restriction fragment analysis with improved probe intensity correction. Nucleic Acids Res. 2014;42:e21.
14. Cawthon RM. Telomere measurement by quantitative PCR. Nucleic Acids Res. 2002;30:e47.
15. Roos G, Kröber A, Grabowski P, et al. Short telomeres are associated with genetic complexity, high-risk genomic aberrations, and short survival in chronic lymphocytic leukemia. Blood 2008;111:2246–2252.
16. Schlegelberger B, Zwingers T, Harder L, et al. Clinicopathogenetic significance of chromosomal abnormalities in patients with blastic peripheral B-cell lymphoma. Blood 1999;94:3114–3120.
17. Schlegelberger B, Metzke S, Harder S, Zühlke-Jenisch R, Zhang Y, Siebert R. Classical and molecular cytogenetics of tumor cells. Springer Lab Manual 1999;11:151–185.
18. Lange K, Holm L, Vang Nielsen K, et al. Telomere shortening and chromosomal instability in myelodysplastic syndromes. Genes Chromosomes Cancer 2010;49:260–269.
19. Baerlocher GM, Vulto I, de Jong G, Lansdorp PM. Flow cytometry and FISH to measure the average length of telomeres (flow FISH). Nat Protoc. 2006;1:2365–2376.
20. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986;1:307–310.
21. Alter BP, Baerlocher GM, Savage SA, et al. 2007. Very short telomere length by flow fluorescence in situ hybridization identifies patients with dyskeratosis congenita. Blood 2007;110:1439–1447.
22. Gutierrez-Rodrigues F, Santana-Lemos BA, Scheucher PS, Alves-Paiva RM, Calado RT. Direct comparison of flow-FISH and qPCR as diagnostic tests for telomere length measurement in humans. PLoS One 2014;9:e113747.
23. Aubert G, Lansdorp PM. Telomeres and aging. Physiol Rev. 2008;88:557–579.
24. Lin J, Epel E, Cheon J, et al. Analyses and comparisons of telomerase activity and telomere length in human T and B cells: insights for epidemiology of telomere maintenance. J Immunol Methods 2010;352:71–80.
25. Nussey DH, Baird D, Barrett E, et al. Measuring telomere length and telomere dynamics in evolutionary biology and ecology. Methods Ecol Evol. 2014;5:299–310.
26. Armanios M, Blackburn EH. The telomere syndromes. Nat Rev Genet. 2012;13:693–704.
27. Gadalla SM, Khincha PP, Katki HA, et al. The limitations of qPCR telomere length measurement in diagnosing dyskeratosis congenita. Mol Genet Genomic Med. 2016;4:475–479.
28. Shimamura A, Alter BP. Pathophysiology and management of inherited bone marrow failure syndromes. Blood Rev. 2010;24:101–122.
29. Savage SA, Bertuch AA. The genetics and clinical manifestations of telomere biology disorders. Genet Med. 2010;12:753–764.
30. Weng NP. Telomere and adaptive immunity. Mech Ageing Dev. 2008;129:60–66.
31. Hodes RJ, Hathcock KS, Weng NP. Telomeres in T and B cells. Nat Rev Immunol. 2002;2:699–706.
32. Barbaro PM, Ziegler DS, Reddel RR. The wide-ranging clinical implications of the short telomere syndromes. Int Med J. 2016;46:393–403.
33. Svenson U, Nordfjall K, Baird D, et al. Blood cell telomere length is a dynamic feature. PLoS One 2011;6:e21485.