Direct comparison of Flow-FISH and qPCR as diagnostic tests for telomere length measurement in humans

PLoS ONE

Volumn 9, Issue 11, 2014, Pages

  Gutierrez Rodrigues, Fernanda ^a,b   Santana Lemos, Barbara A ^a,b   Scheucher, Priscila S ^a,b   Alves Paiva, Raquel M ^a,b   Calado, Rodrigo T ^a,b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b Center for Cell based Therapy São Paulo Research Foundation FAPESP   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; AGED; ANALYTIC METHOD; ARTICLE; BONE MARROW DEPRESSION; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATIONAL STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; FEMALE; FIBROSING ALVEOLITIS; FLOW FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE IN SITU HYBRIDIZATION; GENETIC PARAMETERS; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LEUKOCYTE; MALE; POLYMERASE CHAIN REACTION; QUANTITATIVE POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TELOMERE LENGTH; TERMINAL RESTRICTION FRAGMENT ANALYSIS; APLASTIC ANEMIA; COMPARATIVE STUDY; CYTOLOGY; DYSKERATOSIS CONGENITA; INFANT; METABOLISM; MIDDLE AGED; NEWBORN; PATHOLOGY; PHYSIOLOGY; PRESCHOOL CHILD; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; TELOMERE; TELOMERE SHORTENING; VERY ELDERLY; YOUNG ADULT;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANEMIA, APLASTIC; CHILD; CHILD, PRESCHOOL; DYSKERATOSIS CONGENITA; FEMALE; HUMANS; IDIOPATHIC PULMONARY FIBROSIS; IN SITU HYBRIDIZATION, FLUORESCENCE; INFANT; INFANT, NEWBORN; LEUKOCYTES; MALE; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TELOMERE; TELOMERE SHORTENING; YOUNG ADULT;

EID: 84915746976     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0113747     Document Type: Article

References (33)

1. Blackburn EH (2001) Switching and signaling at the telomere. Cell 106: 661-673.
2. Calado RT, Young NS (2009) Telomere diseases. N Engl J Med 361: 2353-2365.
3. Alter BP, Baerlocher GM, Savage SA, Chanock SJ, Weksler BB, et al. (2007) Very short telomere length by flow fluorescence in situ hybridization identifies patients with dyskeratosis congenita. Blood 110: 1439-1447.
4. Baird DM (2005) New developments in telomere length analysis. Exp Gerontol 40: 363-368.
5. Aubert G, Hills M, Lansdorp PM (2012) Telomere length measurement-caveats and a critical assessment of the available technologies and tools. Mutat Res 730: 59-67.
6. Harley CB, Futcher AB, Greider CW (1990) Telomeres shorten during ageing of human fibroblasts. Nature 345: 458-460.
7. Allshire RC, Dempster M, Hastie ND (1989) Human telomeres contain at least three types of G-rich repeat distributed non-randomly. Nucleic Acids Res 17: 4611-4627.
8. Moyzis RK, Buckingham JM, Cram LS, Dani M, Deaven LL, et al. (1988) A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci U S A 85: 6622-6626.
9. Rufer N, Dragowska W, Thornbury G, Roosnek E, Lansdorp PM (1998) Telomere length dynamics in human lymphocyte subpopulations measured by flow cytometry. Nat Biotechnol 16: 743-747.
10. Baerlocher GM, Vulto I, de Jong G, Lansdorp PM (2006) Flow cytometry and FISH to measure the average length of telomeres (flow FISH). Nat Protoc 1: 2365-2376.
11. Calado RT (2009) Telomeres and marrow failure. Hematology Am Soc Hematol Educ Program: 338-343.
12. Cawthon RM (2002) Telomere measurement by quantitative PCR. Nucleic Acids Res 30: e47.
13. Yamaguchi H, Calado RT, Ly H, Kajigaya S, Baerlocher GM, et al. (2005) Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia. N Engl J Med 352: 1413-1424.
14. Rufer N, Brummendorf TH, Kolvraa S, Bischoff C, Christensen K, et al. (1999) Telomere fluorescence measurements in granulocytes and T lymphocyte subsets point to a high turnover of hematopoietic stem cells and memory T cells in early childhood. J Exp Med 190: 157-167.
15. Baerlocher GM, Mak J, Tien T, Lansdorp PM (2002) Telomere length measurement by fluorescence in situ hybridization and flow cytometry: tips and pitfalls. Cytometry 47: 89-99.
16. Kapoor V, Telford WG (2004) Telomere length measurement by fluorescence in situ hybridization and flow cytometry. Methods Mol Biol 263: 385-398.
17. Rufer N, Brümmendorf TH, Kolvraa S, Bischoff C, Christensen K, et al. (1999) Telomere fluorescence measurements in granulocytes and T lymphocyte subsets point to a high turnover of hematopoietic stem cells and memory T cells in early childhood. J Exp Med 190: 157-167.
18. Scheinberg P, Cooper JN, Sloand EM, Wu CO, Calado RT, et al. (2010) Association of telomere length of peripheral blood leukocytes with hematopoietic relapse, malignant transformation, and survival in severe aplastic anemia. JAMA 304: 1358-1364.
19. Calado RT, Cooper JN, Padilla-Nash HM, Sloand EM, Wu CO, et al. (2012) Short telomeres result in chromosomal instability in hematopoietic cells and precede malignant evolution in human aplastic anemia. Leukemia 26: 700-707.
20. Brouilette SW, Moore JS, McMahon AD, Thompson JR, Ford I, et al. (2007) Telomere length, risk of coronary heart disease, and statin treatment in the West of Scotland Primary Prevention Study: a nested case-control study. Lancet 369: 107-114.
21. Bland JM, Altman DG (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1: 307-310.
22. Hanneman SK (2008) Design, analysis, and interpretation of method-comparison studies. AACN Adv Crit Care 19: 223-234.
23. Aviv A, Hunt SC, Lin J, Cao X, Kimura M, et al. (2011) Impartial comparative analysis of measurement of leukocyte telomere length/DNA content by Southern blots and qPCR. Nucleic Acids Res 39: e134.
24. Elbers CC, Garcia ME, Kimura M, Cummings SR, Nalls MA, et al. (2014) Comparison between southern blots and qPCR analysis of leukocyte telomere length in the health ABC study. J Gerontol A Biol Sci Med Sci 69: 527-531.
25. Pavesi E, Avondo F, Aspesi A, Quarello P, Rocci A, et al. (2009) Analysis of telomeres in peripheral blood cells from patients with bone marrow failure. Pediatr Blood Cancer 53: 411-416.
26. Nishio N, Kojima S (2010) Recent progress in dyskeratosis congenita. Int J Hematol 92: 419-424.
27. Gadalla SM, Savage SA (2011) Telomere biology in hematopoiesis and stem cell transplantation. Blood Rev 25: 261-269.
28. Hultdin M, Grönlund E, Norrback K, Eriksson-Lindström E, Just T, et al. (1998) Telomere analysis by fluorescence in situ hybridization and flow cytometry. Nucleic Acids Res 26: 3651-3656.
29. Cawthon RM (2009) Telomere length measurement by a novel monochrome multiplex quantitative PCR method. Nucleic Acids Res 37: e21.
30. Bischoff C, Petersen HC, Graakjaer J, Andersen-Ranberg K, Vaupel JW, et al. (2006) No association between telomere length and survival among the elderly and oldest old. Epidemiology 17: 190-194.
31. Shen J, Terry MB, Gurvich I, Liao Y, Senie RT, et al. (2007) Short telomere length and breast cancer risk: a study in sister sets. Cancer Res 67: 5538-5544.
32. De Vivo I, Prescott J, Wong JY, Kraft P, Hankinson SE, et al. (2009) A prospective study of relative telomere length and postmenopausal breast cancer risk. Cancer Epidemiol Biomarkers Prev 18: 1152-1156.
33. Willeit P, Willeit J, Brandstatter A, Ehrlenbach S, Mayr A, et al. (2010) Cellular aging reflected by leukocyte telomere length predicts advanced atherosclerosis and cardiovascular disease risk. Arterioscler Thromb Vasc Biol 30: 1649-1656.