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1
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84955326227
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Assembly of the Caenorhabditis elegans gut microbiota from diverse soil microbial environments
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This study characterized the assembly of the gut microbiota in worms of the standard N2 lab starin raised in diverse microcosm environments, and demonstrated that microbiota assembly is a deterministic process shaped to a significant degree by the host.
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1•• Berg, M., Stenuit, B., Ho, J., Wang, A., Parke, C., Knight, M., Alvarez-Cohen, L., Shapira, M., Assembly of the Caenorhabditis elegans gut microbiota from diverse soil microbial environments. ISME J. 10 (2016), 1998–2009 This study characterized the assembly of the gut microbiota in worms of the standard N2 lab starin raised in diverse microcosm environments, and demonstrated that microbiota assembly is a deterministic process shaped to a significant degree by the host.
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2
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This study compared assembly of the gut microbiota in C. elegans and in related strains and species, demonstrating a significant contribution of host genetics to microbiota composition, but also a dominant contribution of environmental diversity. Functional evaluation of gut commensals from two related Caenorhabditis species further showed that commensals from one host provided infection resistance to their cognate host, but not to the other, indicating host-specific adaptations and a greater role of host genetics in shaping microbiota composition than could be discerned using phylogenetic sequence-based analysis alone.
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2•• Berg, M., Zhou, X.Y., Shapira, M., Host-specific functional significance of Caenorhabditis gut commensals. Front. Microbiol., 7, 2016, 1622 This study compared assembly of the gut microbiota in C. elegans and in related strains and species, demonstrating a significant contribution of host genetics to microbiota composition, but also a dominant contribution of environmental diversity. Functional evaluation of gut commensals from two related Caenorhabditis species further showed that commensals from one host provided infection resistance to their cognate host, but not to the other, indicating host-specific adaptations and a greater role of host genetics in shaping microbiota composition than could be discerned using phylogenetic sequence-based analysis alone.
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Berg, M.1
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The native microbiome of the nematode Caenorhabditis elegans: gateway to a new host–microbiome model
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This study characterized microbiotas in C. elegans isolated from the wild, comparing womr microbiotas to those in their environment, and isolating commensals that were subsequently tested for their effects on host fitness. This revealed both positive and negative contributions of commensals to worm proliferation, and further identified Pseudomonas commensals that conferred anti-fungal resistance.
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3•• Dirksen, P., Marsh, S.A., Braker, I., Heitland, N., Wagner, S., Nakad, R., Mader, S., Petersen, C., Kowallik, V., Rosenstiel, P., et al. The native microbiome of the nematode Caenorhabditis elegans: gateway to a new host–microbiome model. BMC Biol. 14 (2016), 1–16 This study characterized microbiotas in C. elegans isolated from the wild, comparing womr microbiotas to those in their environment, and isolating commensals that were subsequently tested for their effects on host fitness. This revealed both positive and negative contributions of commensals to worm proliferation, and further identified Pseudomonas commensals that conferred anti-fungal resistance.
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Garigan, D., Hsu, A.L., Fraser, A.G., Kamath, R.S., Ahringer, J., Kenyon, C., Genetic analysis of tissue aging in Caenorhabditis elegans: a role for heat-shock factor and bacterial proliferation. Genetics 161 (2002), 1101–1112.
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Szewczyk, N.J., Udranszky, I.A., Kozak, E., Sunga, J., Kim, S.K., Jacobson, L.A., Conley, C.A., Delayed development and lifespan extension as features of metabolic lifestyle alteration in C. elegans under dietary restriction. J. Exp. Biol. 209 (2006), 4129–4139.
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Lynn, D.A., Dalton, H.M., Sowa, J.N., Wang, M.C., Soukas, A.A., Curran, S.P., Omega-3 and -6 fatty acids allocate somatic and germline lipids to ensure fitness during nutrient and oxidative stress in Caenorhabditis elegans. Proc. Natl. Acad. Sci. U. S. A., 112, 2015, 201514012.
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Khanna, A., Kumar, J., Vargas, M.A., Barrett, L., Katewa, S., Li, P., McCloskey, T., Sharma, A., Naudé, N., Nelson, C., et al. A genome-wide screen of bacterial mutants that enhance dauer formation in C. elegans. Sci. Rep., 6, 2016, 38764.
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Excessive folate synthesis limits lifespan in the C. elegans: E. coli aging model
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This study identified and characterized an E. coli mutant that was defective in folate biosynthesis and extended C. elegans lifespan, providing the first evidence for regulation of host life history by bacterial metabolites.
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11• Virk, B., Correia, G., Dixon, D.P., Feyst, I., Jia, J., Oberleitner, N., Briggs, Z., Hodge, E., Edwards, R., Ward, J., et al. Excessive folate synthesis limits lifespan in the C. elegans: E. coli aging model. BMC Biol., 10, 2012, 67 This study identified and characterized an E. coli mutant that was defective in folate biosynthesis and extended C. elegans lifespan, providing the first evidence for regulation of host life history by bacterial metabolites.
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Yen, C.A., Curran, S.P., Gene–diet interactions and aging in C. elegans. Exp. Gerontol., 2016, 10.1016/j.exger.2016.02.012.
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Virk, B., Jia, J., Maynard, C.A., Raimundo, A., Lefebvre, J., Richards, S.A., Chetina, N., Liang, Y., Helliwell, N., Cipinska, M., et al. Folate acts in E. coli to accelerate C. elegans aging independently of bacterial biosynthesis. Cell Rep. 14 (2016), 1611–1620.
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Chaudhari, S.N., Mukherjee, M., Vagasi, A.S., Bi, G., Rahman, M.M., Nguyen, C.Q., Paul, L., Selhub, J., Kipreos, E.T., Bacterial folates provide an exogenous signal for C. elegans germline stem cell proliferation. Dev. Cell 38 (2016), 33–46.
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Donato, V., Ayala, F.R., Cogliati, S., Bauman, C., Costa, J.G., Leñini, C., Grau, R., Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway. Nat. Commun., 8, 2017, 14332.
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