Time and temperature affect glycolysis in blood samples regardless of fluoride-based preservatives: a potential underestimation of diabetes

Annals of Clinical Biochemistry

Volumn 54, Issue 6, 2017, Pages 671-676

  Stapleton, Mary ^a   Daly, Niamh ^b   O’Kelly, Ruth ^a   Turner, Michael J ^b  

^a Tallaght and Coombe Women's and Infant's University Hospital   (Ireland)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

Clinical studies; diabetes; laboratory methods

Indexed keywords

EDETIC ACID; FLUORIDE; GLUCOSE; LITHIUM; PRESERVATIVE;

ADULT; ARTICLE; BLOOD SAMPLING; DIET RESTRICTION; FEMALE; GLUCOSE BLOOD LEVEL; GLUCOSE TOLERANCE TEST; GLYCOLYSIS; HUMAN; INCUBATION TEMPERATURE; LABORATORY TECHNIQUE; MAJOR CLINICAL STUDY; PREGNANCY DIABETES MELLITUS; PRIORITY JOURNAL; ROOM TEMPERATURE; VENOUS BLOOD; BLOOD; BLOOD ANALYSIS; DIABETES, GESTATIONAL; DRUG EFFECTS; FALSE NEGATIVE RESULT; METABOLISM; PREGNANCY; PROCEDURES; TEMPERATURE; TIME FACTOR;

BLOOD CHEMICAL ANALYSIS; BLOOD SPECIMEN COLLECTION; DIABETES, GESTATIONAL; FALSE NEGATIVE REACTIONS; FEMALE; FLUORIDES; GLUCOSE TOLERANCE TEST; GLYCOLYSIS; HUMANS; PREGNANCY; TEMPERATURE; TIME FACTORS;

EID: 85020619638     PISSN: 00045632     EISSN: 17581001     Source Type: Journal    
DOI: 10.1177/0004563216682978     Document Type: Article

References (14)

1. International association of Diabetes and Pregnancy Study Groups Consensus Panel. International association of diabetes and pregnancy study groups recommendations on the diagnosis and classification of hyperglycaemia in pregnancy. Diabetes Care 2010; 33: 676–682.
2. HAPO Consultative Study Group. Hyperglycaemia and adverse pregnancy outcomes. N Eng J Med 2008; 358: 1991–2002.
3. Mikesh LM, Bruns DE. Stabilisation of glucose in blood specimens: mechanism of delay in fluoride inhibition of glycolysis. Clin Chem 2008; 54: 930–931.
4. Bruns DE, Knowler WC. Stabilisation of glucose in blood samples: why it matters. Clin Chem 2009; 55: 850–852.
5. Gambino R, Bruns DE. Stabilization of Glucose in Blood Samples: out with the old, in with the new. Clin Chem Lab Med 2013; 51: 1883–1885.
6. The National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines. Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Washington, DC: National Academy of Clinical Biochemistry
7. Daly N, Stapleton M, O'Kelly R, The role of preanalytical glycolysis in the diagnosis of gestational diabetes in obese women. Am J Obs Gynaecol 2015; 213: 84 e1–5.
8. van den Berg SAA, Thelen MHM, Salden LPW, It takes acid, rather than ice, to freeze glucose. Sci Rep 2015; 5: 8875–8875.
9. van der Hagen EAE, Kleefman AMD, Thelen MHM, et al. Normalisation issues in glucose measurements using phlebotomy tubes with liquid additives. Clin Chem Lab Med 2017; 55: e1–3
10. Gambino R, Piscitelli J, Ackattupathil TA, Acidification of blood is superior to sodium fluoride alone as an inhibitor of glycolysis. Clin Chem 2009; 55: 1019–1021.
11. Daly N, Flynn I, Carroll C, Comparison of citrate-fluoride-EDTA with fluoride-EDTA additives to stabilize plasma glucose measurements in women being screened during pregnancy with an oral glucose tolerance test: a prospective observational study. Clin Chem 2016; 62: 886–887.
12. del Pino IG, Constanso I, Mourín LV, Citric/citrate buffer: an effective antiglycolytic agent. Clin Chem Lab Med 2013; 51: 1943–1949.
13. Norman M, Jones I. The shift from fluoride/oxalate to acid citrate/fluoride blood collection tubes for glucose testing – the impact upon patient results. Clin Biochem 2014; 47: 683–685.
14. Yagmur E, van Helden J, Koch A, Effective inhibition of glycolysis in venous whole blood and plasma samples. J Lab Med 2012; 36: 169–177.