It takes acid, rather than ice, to freeze glucose

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Van Den Berg, S A A ^a   Thelen, M H M ^a   Salden, L P W ^a   Van Thiel, S W ^a   Boonen, K J M ^a  

^a AMPHIA HOSPITAL   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CITRIC ACID; GLUCOSE BLOOD LEVEL; ICE; SODIUM FLUORIDE;

ANALYSIS; ARTIFACT; BLOOD SAMPLING; CHEMISTRY; DRUG EFFECTS; FEMALE; GLUCOSE BLOOD LEVEL; GLYCOLYSIS; HUMAN; MALE; PROCEDURES;

ARTIFACTS; BLOOD GLUCOSE; BLOOD SPECIMEN COLLECTION; CITRATES; FEMALE; GLYCOLYSIS; HUMANS; ICE; MALE; SODIUM FLUORIDE;

EID: 84924405779     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep08875     Document Type: Article

References (15)

1. Kehl, K. A.Moving toward peace: an analysis of the concept of a good death. Am J Hosp Palliat Care 23, 277-286 (2006).
2. Sacks, D. B. A1c versus glucose testing: a comparison. Diabetes Care 34, 518-523 (2011).
3. Sacks, D. B. et al. Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Diabetes Care 34, e61-e99 (2011).
4. Weismann, M. & Klein, B. Evaluation of glucose determinations in untreated serum samples. Clin Chem 4, 420-422 (1958).
5. Chan, A. Y., Swaminathan, R.&Cockram, C. S. Effectiveness of sodium fluoride as a preservative of glucose in blood. Clin Chem 35, 315-317 (1989).
6. Field, J. B. &Williams, H. E. Artifactual hypoglycemia associated with leukemia. N Engl J Med 265, 946-948 (1961).
7. de Vegt, F. et al. Relation of impaired fasting and postload glucose with incident type 2 diabetes in a dutch population. J Am Med Assoc 285, 2109-2113 (2001).
8. Fernandez, L. et al. A comparison of glucose concentration in paired specimens collected in serum separator and uoride/potassium oxalate blood collection tubes under survey 'field' conditions. Clin Biochem 46, 285-288 (2013).
9. Qin, J., Chai, G., Brewer, J. M., Lovelace, L. L. & Lebioda, L. Fluoride inhibition of enolase: crystal structure and thermodynamics. Biochemistry 45, 793-800 (2006).
10. van den Berg, S. A. A., Thelen, M. H. M. & Boonen, K. J. Inventarisatie van de (pre)analytische aspecten van de glucose bepaling in nederlandse laboratoria; tijd voor uniformiteit. Ned Tijdschr Klin Chem Labgeneesk 40, 68-71 (2015).
11. Gambino, R. & Bruns, D. E. Stabilization of glucose in blood samples: out with the old, in with the new. Clin Chem and Lab Med 51, 1883-1885 (2013).
12. Gambino, R. et al. Acidification of blood is superior to sodium fluoride alone as an inhibitor of glycolysis. Clin Chem and Lab Med 55, 1019-1021 (2009).
13. Ji, J. Z. & Meng, Q. H. Evaluation of the interference of hemoglobin, bilirubin, and lipids on roche cobas 6000 assays. Clin Chim Acta 412, 17-18 (2011).
14. Lin, Y. L., Smith, C. H. & Dietzler, D. N. Stabilization of blood glucose by cooling with ice: an effective procedure for preservation of samples from adults and newborns. Clin Chem 22, 2031-2033 (1976).
15. Mikesh, L. M. & Bruns, D. E. Stabilization of glucose in blood specimens: Mechanism of delay in fluoride inhibition of glycolysis. Clin Chem 54, 930-932 (2008).