MRNA-mediated glycoengineering ameliorates deficient homing of human stem cell-derived hematopoietic progenitors

Journal of Clinical Investigation

Volumn 127, Issue 6, 2017, Pages 2433-2437

  Lee, Jungmin ^a,b   Dykstra, Brad ^c,d   Spencer, Joel A ^d,e   Kenney, Laurie L ^f   Greiner, Dale L ^f   Shultz, Leonard D ^g   Brehm, Michael A ^f   Lin, Charles P ^e   Sackstein, Robert ^c,d   Rossi, Derrick J ^a,b  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b Harvard University   (United States)

^c BRIGHAM AND WOMEN S HOSPITAL   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

^e Center for Systems Biology and Wellman Center for Photomedicine   (United States)

^f UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^g JACKSON LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; BETA1 INTEGRIN; CHEMOKINE RECEPTOR CXCR4; ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE 1; FIBRONECTIN; FUCOSYLTRANSFERASE; FUCOSYLTRANSFERASE 6; HERMES ANTIGEN; MESSENGER RNA; SIALYL LEWIS X ANTIGEN; STROMAL CELL DERIVED FACTOR 1; UNCLASSIFIED DRUG; VERY LATE ACTIVATION ANTIGEN 4; VERY LATE ACTIVATION ANTIGEN 5; FUT6 PROTEIN, HUMAN; SELE PROTEIN, HUMAN;

ARTICLE; BONE MARROW; CELL DIFFERENTIATION; CELL HOMING; CELL MIGRATION; CELL SURFACE; CONTROLLED STUDY; ENDOTHELIUM; EXTRAVASATION; FUCOSYLATION; GENETIC TRANSFECTION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; INDUCED PLURIPOTENT STEM CELL; MOUSE; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; STROMA CELL; ANIMAL; CELL CULTURE; CELL MOTION; COCULTURE; GENETICS; GLYCOSYLATION; HEMATOPOIETIC STEM CELL TRANSPLANTATION; METABOLISM; NONOBESE DIABETIC MOUSE; PHYSIOLOGY; RNA PROCESSING; SCID MOUSE;

ANIMALS; CELL DIFFERENTIATION; CELL MOVEMENT; CELLS, CULTURED; COCULTURE TECHNIQUES; E-SELECTIN; FUCOSYLTRANSFERASES; GLYCOSYLATION; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MICE, INBRED NOD; MICE, SCID; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, MESSENGER;

EID: 85020195785     PISSN: 00219738     EISSN: 15588238     Source Type: Journal    
DOI: 10.1172/JCI92030     Document Type: Article

References (17)

1. Gratwohl A, et al. Hematopoietic stem cell transplantation: a global perspective. JAMA. 2010;303(16):1617-1624.
2. Lee J, Dykstra B, Sackstein R, Rossi DJ. Progress and obstacles towards generating hematopoietic stem cells from pluripotent stem cells. Curr Opin Hematol. 2015;22(4):317-323.
3. Sackstein R. The lymphocyte homing receptors: gatekeepers of the multistep paradigm. Curr Opin Hematol. 2005;12(6):444-450.
4. Ledran MH, et al. Efficient hematopoietic differentiation of human embryonic stem cells on stromal cells derived from hematopoietic niches. Cell Stem Cell. 2008;3(1):85-98.
5. Nakajima-Takagi Y, et al. Role of SOX17 in hematopoietic development from human embryonic stem cells. Blood. 2013;121(3):447-458.
6. Sturgeon CM, Ditadi A, Awong G, Kennedy M, Keller G. Wnt signaling controls the specification of definitive and primitive hematopoiesis from human pluripotent stem cells. Nat Biotechnol. 2014;32(6):554-561.
7. Mandal PK, Rossi DJ. Reprogramming human fibroblasts to pluripotency using modified mRNA. Nat Protoc. 2013;8(3):568-582.
8. Avigdor A, et al. CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow. Blood. 2004;103(8):2981-2989.
9. Lapidot T, Dar A, Kollet O. How do stem cells find their way home? Blood. 2005;106(6):1901-1910.
10. Su Y, et al. Relating conformation to function in integrin α5β1. Proc Natl Acad Sci USA. 2016;113(27):E3872-E3881.
11. Peled A, et al. The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34(+) cells: role in transendothelial/ stromal migration and engraftment of NOD/ SCID mice. Blood. 2000;95(11):3289-3296.
12. Sackstein R. Glycosyltransferase-programmed stereosubstitution (GPS) to create HCELL: engineering a roadmap for cell migration. Immunol Rev. 2009;230(1):51-74.
13. Gori JL, et al. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells. J Clin Invest. 2015;125(3):1243-1254.
14. Dykstra B, et al. Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells. Stem Cells. 2016;34(10):2501-2511.
15. Robinson SN, et al. Fucosylation with fucosyltransferase VI or fucosyltransferase VII improves cord blood engraftment. Cytotherapy. 2014;16(1):84-89.
16. Wan X, et al. Fucosyltransferase VII improves the function of selectin ligands on cord blood hematopoietic stem cells. Glycobiology. 2013;23(10):1184-1191.
17. Popat U, et al. Enforced fucosylation of cord blood hematopoietic cells accelerates neutrophil and platelet engraftment after transplantation. Blood. 2015;125(19):2885-2892.