Novel multiplex PCR assay for detection of chlorhexidine-quaternary ammonium, mupirocin, and methicillin resistance genes, with simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci

Journal of Clinical Microbiology

Volumn 55, Issue 6, 2017, Pages 1857-1864

  McClure, Jo Ann ^a   DeLongchamp, Johanna Zaal ^a   Conly, John M ^a,b,c   Zhang, Kunyan ^a,b,c  

^a CALGARY LABORATORY SERVICES   (Canada)

^b UNIVERSITY OF CALGARY   (Canada)

^c UNIVERSITY OF CALGARY   (Canada)

Author keywords

Antiseptic; Chlorhexidine; Coagulase negative staphylococci; Methicillin; MRSA; Multiplex PCR; Mupirocin; Quaternary ammonium; Resistance

Indexed keywords

CHLORHEXIDINE; METICILLIN; PSEUDOMONIC ACID; QUATERNARY AMMONIUM DERIVATIVE; RNA 16S; ANTIINFECTIVE AGENT;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; GENE IDENTIFICATION; MEASUREMENT ACCURACY; MECA GENE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN SUSCEPTIBLE STAPHYLOCOCCUS AUREUS; MULTIPLEX POLYMERASE CHAIN REACTION; MUPA GENE; MUPB GENE; NONHUMAN; NUC GENE; PRIORITY JOURNAL; QAC GENE; SENSITIVITY AND SPECIFICITY; SMR GENE; STAPHYLOCOCCUS AUREUS; CLASSIFICATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR DIAGNOSIS; PROCEDURES; STAPHYLOCOCCUS; STAPHYLOCOCCUS INFECTION;

ANTI-BACTERIAL AGENTS; CHLORHEXIDINE; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; HUMANS; METHICILLIN; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; MUPIROCIN; QUATERNARY AMMONIUM COMPOUNDS; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS;

EID: 85019558550     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02488-16     Document Type: Article

References (33)

1. Datta R, Huang SS. 2008. Risk of infection and death due to methicillin-resistant Staphylococcus aureus in long-term carriers. Clin Infect Dis 47:176-181. https://doi.org/10.1086/589241.
2. Weber SG, Huang SS, Oriola S, Huskins WC, Noskin GA, Harriman K, Olmsted RN, Bonten M, Lundstrom T, Climo MW, Roghmann MC, Murphy CL, Karchmer TB. 2007. Legislative mandates for use of active surveillance cultures to screen for methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci: position statement from the Joint SHEA and APIC Task Force. Am J Infect Control 35:73-85. https://doi.org/10.1016/j.ajic.2007.01.001.
3. Merrer J, Santoli F, Appere de Vecchi C, Tran B, De Jonghe B, Outin H. 2000. "Colonization pressure" and risk of acquisition of methicillin-resistant Staphylococcus aureus in a medical intensive care unit. Infect Control Hosp Epidemiol 21:718-723. https://doi.org/10.1086/501721.
4. Appelbaum PC. 2007. Microbiology of antibiotic resistance in Staphylococcus aureus. Clin Infect Dis 45(Suppl 3):S165-S170. https://doi.org/10.1086/519474.
5. Boyce JM. 1996. Preventing staphylococcal infections by eradicating nasal carriage of Staphylococcus aureus: proceeding with caution. Infect Control Hosp Epidemiol 17:775-779.
6. Conly JM, Vas S. 2002. Increasing mupirocin resistance of Staphylococcus aureus in CAPD - should it continue to be used as prophylaxis? Perit Dial Int 22:649-652.
7. Cookson BD. 1998. The emergence of mupirocin resistance: a challenge to infection control and antibiotic prescribing practice. J Antimicrob Chemother 41:11-18. https://doi.org/10.1093/jac/41.1.11.
8. Farr BM. 2002. Mupirocin to prevent S. aureus infections. N Engl J Med 346:1905-1906. https://doi.org/10.1056/NEJMed020048.
9. Miyazaki NH, Abreu AO, Marin VA, Rezende CA, Moraes MT, Villas Boas MH. 2007. The presence of qacA/B gene in Brazilian methicillin-resistant Staphylococcus aureus. Mem Inst Oswaldo Cruz 102:539-540. https://doi.org/10.1590/S0074-02762007000400018.
10. Simor AE, Stuart TL, Louie L, Watt C, Ofner-Agostini M, Gravel D, Mulvey M, Loeb M, McGeer A, Bryce E, Matlow A. 2007. Mupirocin-resistant, methicillin-resistant Staphylococcus aureus strains in Canadian hospitals. Antimicrob Agents Chemother 51:3880-3886. https://doi.org/10.1128/AAC.00846-07.
11. Vivoni AM, Santos KR, de-Oliveira MP, Giambiagi-deMarval M, Ferreira AL, Riley LW, Moreira BM. 2005. Mupirocin for controlling methicillin-resistant Staphylococcus aureus: lessons from a decade of use at a university hospital. Infect Control Hosp Epidemiol 26:662-667. https://doi.org/10.1086/502599.
12. Wang JT, Sheng WH, Wang JL, Chen D, Chen ML, Chen YC, Chang SC. 2008. Longitudinal analysis of chlorhexidine susceptibilities of nosocomial methicillin-resistant Staphylococcus aureus isolates at a teaching hospital in Taiwan. J Antimicrob Chemother 62:514-517. https://doi.org/10.1093/jac/dkn208.
13. Hodgson JE, Curnock SP, Dyke KG, Morris R, Sylvester DR, Gross MS. 1994. Molecular characterization of the gene encoding high-level mupirocin resistance in Staphylococcus aureus J2870. Antimicrob Agents Chemother 38:1205-1208. https://doi.org/10.1128/AAC.38.5.1205.
14. Seah C, Alexander DC, Louie L, Simor A, Low DE, Longtin J, Melano RG. 2012. MupB, a new high-level mupirocin resistance mechanism in Staphylococcus aureus. Antimicrob Agents Chemother 56:1916-1920. https://doi.org/10.1128/AAC.05325-11.
15. Grinius L, Dreguniene G, Goldberg EB, Liao CH, Projan SJ. 1992. A staphylococcal multidrug resistance gene product is a member of a new protein family. Plasmid 27:119-129. https://doi.org/10.1016/0147-619X(92)90012-Y.
16. Littlejohn TG, DiBerardino D, Messerotti LJ, Spiers SJ, Skurray RA. 1991. Structure and evolution of a family of genes encoding antiseptic and disinfectant resistance in Staphylococcus aureus. Gene 101:59-66. https://doi.org/10.1016/0378-1119(91)90224-Y.
17. Paulsen IT, Brown MH, Skurray RA. 1996. Proton-dependent multidrug efflux systems. Microbiol Rev 60:575-608.
18. Huang SS, Septimus E, Kleinman K, Moody J, Hickok J, Avery TR, Lankiewicz J, Gombosev A, Terpstra L, Hartford F, Hayden MK, Jernigan JA, Weinstein RA, Fraser VJ, Haffenreffer K, Cui E, Kaganov RE, Lolans K, Perlin JB, Platt R. 2013. Targeted versus universal decolonization to prevent ICU infection. N Engl J Med 368:2255-2265. https://doi.org/10.1056/NEJMoa1207290.
19. REDUCE MRSA Trial Working Group, Harvard Pilgrim Health Care, University of California - Irvine, Hospital Corporation of America. 2013. Universal ICU decolonization: an enhanced protocol. Agency for Healthcare Research and Quality, US Department of Health and Human Services, Rockville, MD. https://www.ahrq.gov/professionals/systems/hospital/universal-icu-decolonization/index.html.
20. Sievert DM, Ricks P, Edwards JR, Schneider A, Patel J, Srinivasan A, Kallen A, Limbago B, Fridkin S. 2013. Antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2009-2010. Infect Control Hosp Epidemiol 34: 1-14. https://doi.org/10.1086/668770.
21. Lee H, Lim H, Bae IK, Yong D, Jeong SH, Lee K, Chong Y. 2013. Coexistence of mupirocin and antiseptic resistance in methicillin-resistant Staphylococcus aureus isolates from Korea. Diagn Microbiol Infect Dis 75:308-312. https://doi.org/10.1016/j.diagmicrobio.2012.11.025.
22. Longtin J, Seah C, Siebert K, McGeer A, Simor A, Longtin Y, Low DE, Melano RG. 2011. Distribution of antiseptic resistance genes qacA, qacB, and smr in methicillin-resistant Staphylococcus aureus isolated in Toronto, Canada, from 2005 to 2009. Antimicrob Agents Chemother 55: 2999-3001. https://doi.org/10.1128/AAC.01707-10.
23. Mc Gann P, Milillo M, Kwak YI, Quintero R, Waterman PE, Lesho E. 2013. Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus. Diagn Microbiol Infect Dis 77:270-272. https://doi.org/10.1016/j.diagmicrobio.2013.06.006.
24. Panda S, Kar S, Choudhury R, Sharma S, Singh DV. 2014. Development and evaluation of hexaplex PCR for rapid detection of methicillin, cadmium/zinc and antiseptic-resistant staphylococci, with simultaneous identification of PVL-positive and -negative Staphylococcus aureus and coagulase negative staphylococci. FEMS Microbiol Lett 352:114-122. https://doi.org/10.1111/1574-6968.12383.
25. May L, Klein EY, Rothman RE, Laxminarayan R. 2014. Trends in antibiotic resistance in coagulase-negative staphylococci in the United States, 1999 to 2012. Antimicrob Agents Chemother 58:1404-1409. https://doi.org/10.1128/AAC.01908-13.
26. Leelaporn A, Paulsen IT, Tennent JM, Littlejohn TG, Skurray RA. 1994. Multidrug resistance to antiseptics and disinfectants in coagulase-negative staphylococci. J Med Microbiol 40:214-220. https://doi.org/10.1099/00222615-40-3-214.
27. Noguchi N, Suwa J, Narui K, Sasatsu M, Ito T, Hiramatsu K, Song JH. 2005. Susceptibilities to antiseptic agents and distribution of antiseptic-resistance genes qacA/B and smr of methicillin-resistant Staphylococcus aureus isolated in Asia during 1998 and 1999. J Med Microbiol 54: 557-565. https://doi.org/10.1099/jmm.0.45902-0.
28. Zhang K, Sparling J, Chow BL, Elsayed S, Hussain Z, Church DL, Gregson DB, Louie T, Conly JM. 2004. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci. J Clin Microbiol 42:4947-4955. https://doi.org/10.1128/JCM.42.11.4947-4955.2004.
29. Jaffe RI, Lane JD, Albury SV, Niemeyer DM. 2000. Rapid extraction from and direct identification in clinical samples of methicillin-resistant staphylococci using the PCR. J Clin Microbiol 38:3407-3412.
30. Shortle D. 1983. A genetic system for analysis of staphylococcal nuclease. Gene 22:181-189.
31. Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. 2005. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 43:5026-5033. https://doi.org/10.1128/JCM.43.10.5026-5033.2005.
32. Anthony RM, Connor AM, Power EG, French GL. 1999. Use of the polymerase chain reaction for rapid detection of high-level mupirocin resistance in staphylococci. Eur J Clin Microbiol Infect Dis 18:30-34. https://doi.org/10.1007/s100960050222.
33. McClure JA, Conly JM, Elsayed S, Zhang K. 2010. Multiplex PCR assay to facilitate identification of the recently described Staphylococcal cassette chromosome mec type VIII. Mol Cell Probes 24:229-232. https://doi.org/10.1016/j.mcp.2010.01.001.