Multiplex PCR assay to facilitate identification of the recently described staphylococcal cassette chromosome mec type VIII

Molecular and Cellular Probes

Volumn 24, Issue 4, 2010, Pages 229-232

  McClure, Jo Ann ^a   Conly, John M ^a,b,c   Elsayed, Sameer ^b   Zhang, Kunyan ^a,b,c  

^a CALGARY LABORATORY SERVICES   (Canada)

^b UNIVERSITY OF CALGARY   (Canada)

^c UNIVERSITY OF CALGARY   (Canada)

Author keywords

Methicillin resistant Staphylococcus aureus (MRSA); Multiplex PCR; Staphylococcal cassette chromosome mec (SCCmec)

Indexed keywords

BACTERIAL PROTEIN; STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPE VIII PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; COAGULASE NEGATIVE STAPHYLOCOCCUS; GENE SEQUENCE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN SUSCEPTIBLE STAPHYLOCOCCUS AUREUS; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

BACTERIAL TYPING TECHNIQUES; BIOLOGICAL ASSAY; CHROMOSOMES, BACTERIAL; INTERSPERSED REPETITIVE SEQUENCES; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; STAPHYLOCOCCUS;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS;

EID: 77954385945     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2010.01.001     Document Type: Article

References (10)

1. IWG-SCC Classification of staphylococcal cassette chromosome mec (SCCmec): guidelines for reporting novel SCCmec elements. Antimicrob Agents Chemother 2009, 53(12):4961-4967.
2. Zhang K., McClure J.A., Elsayed S., Conly J.M. Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2009, 53(2):531-540.
3. Oliveira D.C., de Lencastre H. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2002, 46(7):2155-2161.
4. Kondo Y., Ito T., Ma X.X., Watanabe S., Kreiswirth B.N., Etienne J., et al. Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother 2007, 51(1):264-274.
5. Zhang K., McClure J.A., Elsayed S., Louie T., Conly J.M. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2005, 43(10):5026-5033.
6. Shore A.C., Rossney A.S., O'Connell B., Herra C.M., Sullivan D.J., Humphreys H., et al. Detection of staphylococcal cassette chromosome mec-associated DNA segments in multiresistant methicillin-susceptible Staphylococcus aureus (MSSA) and identification of Staphylococcus epidermidis ccrAB4 in both methicillin-resistant S. aureus and MSSA. Antimicrob Agents Chemother 2008, 52(12):4407-4419.
7. Chen L., Mediavilla J.R., Oliveira D.C., Willey B.M., de Lencastre H., Kreiswirth B.N. Multiplex real-time PCR for rapid staphylococcal cassette chromosome mec typing. J Clin Microbiol 2009, 47(11):3692-3706.
8. Zhang K., Sparling J., Chow B.L., Elsayed S., Hussain Z., Church D.L., et al. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci. J Clin Microbiol 2004, 42(11):4947-4955.
9. Ito T., Katayama Y., Asada K., Mori N., Tsutsumimoto K., Tiensasitorn C., et al. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2001, 45(5):1323-1336.
10. Ito T., Ma X.X., Takeuchi F., Okuma K., Yuzawa H., Hiramatsu K. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrob Agents Chemother 2004, 48(7):2637-2651.