Rapid extraction from and direct identification in clinical samples of methicillin-resistant staphylococci using the PCR

Journal of Clinical Microbiology

Volumn 38, Issue 9, 2000, Pages 3407-3412

  Jaffe, R I ^a   Lane, J D ^a   Albury, S V ^a   Niemeyer, D M ^a  

^a Commonwealth Biotechnologies Inc ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ARTICLE; BACTEREMIA; BACTERIUM IDENTIFICATION; BLOOD CULTURE; BLOOD SAMPLING; DNA TEMPLATE; EXTRACTION; GENE SEQUENCE; HOSPITAL INFECTION; LABORATORY TEST; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; POSIBACTERIA; STAPHYLOCOCCI; STAPHYLOCOCCUS AUREUS;

EID: 0033827782     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.9.3407-3412.2000     Document Type: Article

References (51)

1. Discrepancies between mecA PCR and conventional tests used for detection of methicillin resistant Staphylococcus aureus
2. Antimicrobial susceptibility of coagulase-negative staphylococci
3. Origin and evolution of DNA associated with resistance to methicillin in staphylococci
4. Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR
5. Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning mecA-specific DNA
6. FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: Molecular cloning and characterization
7. Rapid detection of the Staphylococcus mecA gene from BACTEC blood culture bottles by the polymerase chain reaction
8. Methicillin resistance in staphylococci: Molecular and biochemical basis and clinical implications
9. Coagulase-negative staphylococci resistant to β-lactam antibiotics in vivo produce penicillin-binding protein 2a
10. An outbreak of infection caused by strains of Staphylococcus aureus resistant to methicillin and aminoglycosides I
11. The epidemiology of methicillin-resistant Staphylococcus aureus infection and colonization
12. The reliability of Microscan conventional and rapid panels to identify Staphylococcus aureus and detect methicillin resistance: An evaluation using the tube coagulase test and mecA PCR
13. Staphylococcus, Micrococcus, and similar organisms
14. Comparison of ATB Staph, Rapid ATB Staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possessing mecA
15. Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory
16. Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus
17. A low-affinity penicillin-binding protein associated with β-lactam resistance in Staphylococcus aureus and molecular cloning of mec-specific DNA
18. Rapid detection of methicillin-resistant staphylococci by multiplex PCR
19. Rapid diagnosis of methicillin-resistant Staphylococcus aureus bacteremia by nested polymerase chain reaction
20. Clinical epidemiologic and bacteriologic observations of an outbreak of methicillin-resistant Staphylococcus aureus at a large community hospital
21. Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative staphylococcus species
22. Branched-DNA assay for detection of the mecA gene in oxacillin-resistant and oxacillin-sensitive staphylococci
23. Methicillin-resistant Staphylococcus aureus: The persistent, resistant nosocomial pathogen
24. Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to β-lactam antibiotics in Staphylococcus aureus
25. The role of β-lactamase in staphylococcal resistance to penicillinase-resistant penicillin and cephalosporin
26. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction
27. Involvement of multiple genetic determinants in high-level methicillin resistance in Staphylococcus aureus
28. Feasibility determination for use of polymerase chain reaction (PCR) in the USAF air transportable hospital (ATH) field environment: Lessons learned
29. Role of mecA transcriptional regulation in the phenotypic expression of methicillin resistance in Staphylococcus aureus
30. Genotypic identification of methicillin-resistant coagulase-negative staphylococci by polymerase chain reaction
31. Identification of the uncultured bacillus of Whipple's disease
32. Misclassification of susceptible strains of Staphylococcus aureus as methicillin-resistant S. aureus by rapid automated susceptibility testing system
33. Correlation between regulation of mecA transcription and expression of methicillin resistance in staphylococci
34. Identification of methicillin-resistant staphylococci by multiplex polymerase chain reaction
35. Penicillinase-negative variants of methicillin-resistant Staphylococcus aureus
36. Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion
37. Survey of methicillin-resistant clinical strains of coagulase-negative staphylococci for mecA gene distribution
38. Comparison of a polymerase chain reaction assay and a conventional microbiologic method for detection of methicillin-resistant Staphylococcus aureus
39. Multiple antibiotic resistant pathogenic bacteria
40. New mechanism for methicillin resistance in Staphylococcus aureus: Clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity
41. Occurrence of a β-lactam-inducible penicillin-binding protein in methicillin-resistant (heteroresistant) staphylococci
42. Detection of methicillin-resistant staphylococci by using the polymerase chain reaction
43. Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphylococcus aureus
44. Rapid and specific molecular identification of methicillin-resistant Staphylococcus aureus in endotracheal aspirates from mechanically ventilated patients
45. Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR
46. Choice of a routine method for detecting methicillin-resistance in staphylococci
47. Comparison of PCR detection of mecA with standard susceptibility testing methods to determine methicillin resistance in coagulase-negative staphylococci
48. Laboratory diagnosis of oxacillin resistance in Staphylococcus aureus by a multiplex-polymerase chain reaction assay