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• Marro A, Bandukwala T, Mak W. Three-dimensional printing and medical imaging: a review of the methods and applications. Curr Probl Diagn Radiol 2015. In this review the authors provide a general overview of the potential uses, process and limitation of 3D printing from medical imaging data including 3D bioprinting.
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• Teo EY, Ong SY, Chong MS, et al. Polycaprolactone-based fused deposition modeled mesh for delivery of antibacterial agents to infected wounds. Biomaterials 2011;32:279-87. This study presented the use of 3D printed antibiotic delivery system used in vivo, although the system was used in mice it is groundbreaking research as it applies 3D printing in a very common pathology to provide clinical improvement and at the same time reducing systemic exposure to antibiotic. It is also one of the earlier uses of 3D printing in vivo where the printed system is not used as structural component to provide a scaffold for the own body to heal, instead it a functional drug delivery system.
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• Ventola CL. Medical Applications for 3D printing: current and projected uses. P T 2014;39:704–11. This article focuses on the current uses of 3D printing in medicine; briefly discussing bioprinitng tissue and organs, custom implants and prostheses, anatomical models for surgical preparation, drug delivery devices (unique dosage forms) and describes some of the current barriers and controversies, including safety, regulatory concerns and potential copyright and patent issues.
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Koch L, Kuhn S, Sorg H, et al. Laser printing of skin cells and human stem cells. Tissue Eng Part C Methods 2010;16:847–54. Laser printing based on laser-induced forward transfer (LIFT) is a new and promising biofabrication technique for the arrangement of biological materials or living cells. In this study LIFT was used to print cell with high potential in regeneration (skin and mesechymal cells) to evaluate the influence of LIFT on the cells. The results showed high transfer rate and no increase of apoptosis or DNA fragmentation. These results show that LIFT will be a promising method for ex vivo cell printing.
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Mandibular reconstruction is one of the most complex reconstructions performed in the wide spectrum of the reconstructive surgery practice. The complex three-dimensional shape, requiring multiple osteotomies that can impair blood flow, the need for enough bone to support implants, occasional need to reconstruct the condyle and the morbidity associated with the donor site (usually fibula) make this a complex issue. This study present 3D printing of artificial bones and implanted them in ten patients with maxillofacial deformities. Findings in this study provide support for further clinical studies of the inkjet-printed custommade artificial bones
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Saijo H, Igawa K, Kanno Y, et al. Maxillofacial reconstruction using custom-made artificial bones fabricated by inkjet printing technology. Journal of artificial organs: the official journal of the Japanese Society for Artificial Organs 2009;12:200–5. Mandibular reconstruction is one of the most complex reconstructions performed in the wide spectrum of the reconstructive surgery practice. The complex three-dimensional shape, requiring multiple osteotomies that can impair blood flow, the need for enough bone to support implants, occasional need to reconstruct the condyle and the morbidity associated with the donor site (usually fibula) make this a complex issue. This study present 3D printing of artificial bones and implanted them in ten patients with maxillofacial deformities. Findings in this study provide support for further clinical studies of the inkjet-printed custommade artificial bones.
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• Levine JP, Patel A, Saadeh PB, Hirsch DL. Computer-aided design and manufacturing in craniomaxillofacial surgery: the new state of the art. The Journal of craniofacial surgery 2012;23:288–93. This paper illustrates a clear clinical advantage in the use of 3D printing as an aid in surgery, in this case, mandibular reconstruction. For bone grafts to be used in mandibular reconstruction there is no disadvantage and many very well defined advantages of using osteotomy guides (that need to be generated with CT reconstructions). Therefore it is ideal for all mandibular reconstruction with free bone graft to use 3D printed osteotomy guides. At the moment there a few of these clear-cut clinical applications of 3D printing in surgery, reason why we find this paper of importance.
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The authors utilized a laser-assisted bioprinting (LaBP) technique to create a fully cellularized skin substitute allowing printing different cell types in a 3D spatial pattern. It was then implanted into full thickness wound of mice. Their results showed tissue formation in vivo on the construct. This technique overcomes a very important hurdle in the journey for 3D printing complex tissues
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• Michael S, Sorg H, Peck CT, et al. Tissue engineered skin substitutes created by laser-assisted bioprinting form skin-like structures in the dorsal skin fold chamber in mice. PLoS One 2013;8:e57741. The authors utilized a laser-assisted bioprinting (LaBP) technique to create a fully cellularized skin substitute allowing printing different cell types in a 3D spatial pattern. It was then implanted into full thickness wound of mice. Their results showed tissue formation in vivo on the construct. This technique overcomes a very important hurdle in the journey for 3D printing complex tissues.
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Michael, S.1
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93
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84901915693
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Blood supply to newly engineered tissues is barrier in transplantation. In this study the authors created vascular networks in hydrogels and demonstrated the functionality of the fabricated vascular networks in improving mass transport, cellular viability and differentiation within the cell-laden tissue constructs. Also formation of endothelial monolayers within the fabricated channels was confirmed. This is a breakthrough in tissue engineering of complex tissues
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• Bertassoni LE, Cecconi M, Manoharan V, et al. Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. Lab Chip 2014;14:2202–11. Blood supply to newly engineered tissues is barrier in transplantation. In this study the authors created vascular networks in hydrogels and demonstrated the functionality of the fabricated vascular networks in improving mass transport, cellular viability and differentiation within the cell-laden tissue constructs. Also formation of endothelial monolayers within the fabricated channels was confirmed. This is a breakthrough in tissue engineering of complex tissues.
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Bertassoni, L.E.1
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94
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Saijo H, Igawa K, Kanno Y, et al. Maxillofacial reconstruction using custom-made artificial bones fabricated by inkjet printing technology. J Artif Organs. 2009;12:200–5.
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