Optimization of L-asparaginase production from novel Enterobacter sp., by submerged fermentation using response surface methodology

Preparative Biochemistry and Biotechnology

Volumn 47, Issue 3, 2017, Pages 219-228

  Erva, Rajeswara Reddy ^a   Goswami, Ajgebi Nath ^a   Suman, Priyanka ^a   Vedanabhatla, Ravali ^a   Rajulapati, Satish Babu ^a  

^a NATIONAL INSTITUTE OF TECHNOLOGY   (India)

Author keywords

antileukemic drug; Enterobacter aerogenes MTCC111; enzyme activity; L Asparaginase; optimization; substrate specificity

Indexed keywords

ANTINEOPLASTIC AGENT; ASPARAGINASE; ASPARAGINE; CARBON; NITROGEN;

BATCH CELL CULTURE; ENTEROBACTER AEROGENES; ENZYME SPECIFICITY; ENZYMOLOGY; FERMENTATION; METABOLISM; MICROBIOLOGY; PROCEDURES; TEMPERATURE;

ANTINEOPLASTIC AGENTS; ASPARAGINASE; ASPARAGINE; BATCH CELL CULTURE TECHNIQUES; CARBON; ENTEROBACTER AEROGENES; FERMENTATION; INDUSTRIAL MICROBIOLOGY; NITROGEN; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 85014899262     PISSN: 10826068     EISSN: 15322297     Source Type: Journal    
DOI: 10.1080/10826068.2016.1201683     Document Type: Article

References (55)

1. Verma, N.,; Kumar, K.,; Kaur, G.,; Anand, S., L-Asparaginase:A Promising Chemotherapeutic Agent. Crit. Rev. Biotechnol. 2007, 27(1), 45–62.
2. Stecher, A.,; De Deus, P.M.,; Polikarpov, I.,; Abrahao-Neto, J., Stability of L-Asparaginase:An Enzyme Used in Leukemia Treatment. Pharm. Acta. Helv. 1999, 74(1), 1–9.
3. Moharam, M.,; Gamal-Eldeen, A.,; El-Sayed, S., Production, Immobilization and Anti-tumor Activity of L-Asparaginase of Bacillus sp R36. J. Am. Sci. 2010, 6(8), 131–140.
4. Hendriksen, H.V.,; Kornbrust, B.A.,; Østergaard, P.R.,; Stringer, M.A., Evaluating the Potential for Enzymatic Acrylamide Mitigation in a Range of Food Products using an Asparaginase from Aspergillus oryzae. J. Agr. Food Chem. 2009, 57(10), 4168–4176.
5. Reynolds, D.,; Taylor, J., The Fungal Holomorph:A Consideration of Mitotic Meiotic and Pleomorphic Speciation. CAB International:Wallingford, UK, 1993.
6. Thomas, X.,; Cannas, G.,; Chelghoum, Y.,; Gougounon, A., Therapeutic Alternatives to Native L-Asparaginase in the Treatment of Adult Acute Lymphoblastic Leukemia. Bull. Cancer 2010, 97(9), 1105–1117.
7. Graham, M.L., Pegaspargase:A Review of Clinical Studies. Adv. Drug Deliv. Rev. 2003, 55(10), 1293–1302.
8. Duval, M.,; Suciu, S.,; Ferster, A.,; Rialland, X.,; Nelken, B.,; Lutz, P.,; Benoit, Y.,; Robert, A.,; Manel, A.M.,; Vilmer, E., Comparison of Escherichia coli–Asparaginase with Erwinia-asparaginase in the Treatment of Childhood Lymphoid Malignancies:Results of a Randomized European Organisation for Research and Treatment of Cancer—Children’s Leukemia Group Phase 3 Trial. Blood. 2002, 99(8), 2734–2749.
9. El-Naggar, NE.A.,; El-Ewasy, S.M.,; El-Shweihy, N.M., Microbial L-Asparaginase as a Potential Therapeutic Agent for the Treatment of Acute Lymphoblastic Leukemia:The Pros and Cons. Int. J. Pharmacol. 2014, 10(4), 182–199.
10. Howard, J.B.,; Carpenter, F.H., L-Asparaginase from Erwinia carotovora Substrate Specificity and Enzymatic Properties. J. Biol. Chem. 1972, 247(4), 1020–1030.
11. Prager, M.D.,; Bachynsky, N., Asparagine Synthetase in Asparaginase Resistant and Susceptible Mouse Lymphomas. Biochem. Bioph. Res. Commun. 1968, 31(1), 43–47.
12. Hawkins, D.S.,; Park, J.R.,; Thomson, B.G.,; Felgenhauer, J.L.,; Holcenberg, J.S.,; Panosyan, E.H.,; Avramis, V.I., Asparaginase Pharmacokinetics After Intensive Polyethylene Glycol-conjugated L-Asparaginase Therapy for Children with Relapsed Acute Lymphoblastic Leukemia. Clin. Cancer Res. 2004, 10(16), 5335–5341.
13. Patro, K.K.R.,; Satpathy, S.,; Gupta, N., Evaluation of Some Fungi for L-Asparaginase Production. Indian J. Fund. Appl. Life Sci. 2011, 1(4), 219–221.
14. Narayana, K.,; Kumar, K.,; Vijayalakshmi, M., L-Asparaginase Production by Streptomyces albidoflavus. Indian J. Microbiol. 2008, 48(3), 331–336.
15. Amena, S.,; Vishalakshi, N.,; Prabhakar, M.,; Dayanand, A.,; Lingappa, K., Production, Purification and Characterization of L-Asparaginase from Streptomyces gulbargensis. Braz. J. Microbiol. 2010, 41(1), 173–178.
16. Pradhan, B.,; Dash, S.K.,; Sahoo, S., Screening and Characterization of Extracelluar L-Asparaginase Producing Bacillus subtilis Strain hswx88, Isolated from Taptapani Hotspring of Odisha, India. Asian Pac. J. Trop. Biomed. 2013, 3(12), 936–941.
17. Hymavathi, M.,; Sathish, T.,; Rao, C.S.,; Prakasham, R., Enhancement of L-Asparaginase Production by Isolated Bacillus circulans (MTCC 8574) using Response Surface Methodology. Appl. Biochem. Biotechnol. 2009, 159(1), 191–198.
18. El-Naggar, N.E.A.,; El-Bindary, A.A.,; Nour, N.S., Production of Antimicrobial Agent Inhibitory to Some Human Pathogenic Multidrug-resistant Bacteria and Candida albicans by Streptomyces sp NEAE-1. Int. J. Pharmacol. 2013, 9(6), 335–347.
19. El-Naggar, N.E.A.,; El-Bindary, A.A.,; Nour, N.S., Statistical Optimization of Process Variables for Antimicrobial Metabolites Production by Streptomyces anulatus NEAE-94 Against Some Multidrug-resistant Strains. Int. J. Pharmacol. 2013, 9(6), 322–334.
20. El-Naggar, N.E.A.,; Abdelwahed, N.A., application of Statistical Experimental Design for Optimization of Silver Nanoparticles Biosynthesis by a Nanofactory Streptomyces viridochromogenes. J. Microbiol. 2014, 52(1), 53–63.
21. El-Naggar, N.E.A.,; Abdelwahed, N.A.,; Darwesh, O.M., Fabrication of Biogenic Antimicrobial Silver Nanoparticles by Streptomyces aegyptia NEAE 102 as Eco-friendly Nanofactory. J. Microbiol. Biotechnol. 2014, 24(4), 453–464.
22. Nawani, N.,; Kapadnis, B., Optimization of Chitinase Production using Statistics Based Experimental Designs. Process Biochem. 2005, 40(2), 651–660.
23. Chang, C.Y.,; Lee, C.L.,; Pan, T.M., Statistical Optimization of Medium Components for the Production of Antrodia cinnamomea AC0623 in Submerged Cultures. Appl. Microbiol. Biotechnol. 2006, 72(4), 654–661.
24. Potumarthi, R.,; Mugeraya, G.,; Jetty, A., Biological Treatment of Toxic Petroleum Spent Caustic in Fluidized Bed Bioreactor using Immobilized Cells of Thiobacillus RAI01. Appl. Biochem. Biotechnol. 2008, 151(2–3), 532–546.
25. Potumarthi, R.,; Subhakar, C.,; Pavani, A.,; Jetty, A., Evaluation of Various Parameters of Calcium-alginate Immobilization Method for Enhanced Alkaline Protease Production by Bacillus licheniformis NCIM-2042 using Statistical Methods. Bioresour. Technol. 2008, 99(6), 1776–1786.
26. Doddapaneni, K.K.,; Tatineni, R.,; Potumarthi, R.,; Mangamoori, L.N., Optimization of Media Constituents Through Response Surface Methodology for Improved Production of alkaline proteases by Serratia rubidaea. J. Chem. Technol. Biotechnol. 2007, 82(8), 721–729.
27. Tatineni, R.,; Doddapaneni, K.K.,; Potumarthi, R.C.,; Mangamoori, L.N., Optimization of Keratinase Production and Enzyme Activity using Response Surface Methodology with Streptomyces sp7. Appl. Biochem. Biotechnol. 2007, 141(2–3), 187–201.
28. Chennupati, S.,; Potumarthi, R.,; Rao, M.G.,; Manga, P.L.,; Sridevi, M.,; Jetty, A., Multiple Responses Optimization and Modeling of Lipase Production by Rhodotorula mucilaginosa MTCC-8737 using Response Surface Methodology. Appl. Biochem. Biotechnol. 2009, 159(2), 317–329.
29. Vellanki, R.N.,; Potumarthi, R.,; Mangamoori, L.N., Constitutive Expression and Optimization of Nutrients for Streptokinase Production by Pichia pastoris using Statistical Methods. Appl. Biochem. Biotechnol. 2009, 158(1), 25–40.
30. Farhat-Khemakhem, A.,; Farhat, M.B.,; Boukhris, I.,; Bejar, W.,; Bouchaala, K.,; Kammoun, R.,; Maguin, E.,; Bejar, S.,; Chouayekh, H., Heterologous Expression and Optimization using Experimental Designs Allowed Highly Efficient Production of the PHY US417 Phytase in Bacillus subtilis 168. AMB Express. 2012, 2(1), 1–11.
31. Mukherjee, J.,; Majumdar, S.,; Scheper, T., Studies on Nutritional and Oxygen Requirements for Production of L-Asparaginase by Enterobacter aerogenes. Appl. Microbiol. Biotechnol. 2000, 53(2), 180–184.
32. Gulati, R.,; Saxena, R.,; Gupta, R., A Rapid Plate Assay for Screening L-Asparaginase Producing Micro-organisms. Lett. Appl. Microbiol. 1997, 24(1), 23–26.
33. Wriston, J.,; Yellin T., L-Asparaginase:A Review. Adv. Enzymol. Relat. Areas Mol. Biol. 1973, 39, 185–248.
34. Lowry, O.H.,; Rosebrough, N.,; Farr, A.L.,; Randall, R.J., Protein Measurement with the Folin Phenol Reagent. J. Biol. Chem. 1951, 193(1), 265–275.
35. Rajulapati, S.B.,; Lakshmi Narasu, M.,; Vundavilli, P., Optimization of α-Amylase Production from Aspergillus niger using Spoiled Starch Rich Vegetables by Response Surface Methodology and Genetic Algorithm. India Conference (INDICON), Annual IEEE. IEEE, BITS Pilani, Hyderabad, 16–18 December, 2011, 1–9.
36. Sharma, A.,; Husain, I., Optimization of Medium Components for Extracellular Glutaminase Free Asparaginase from Enterobacter cloacae. Int. J. Curr. Microbiol. Appl. Sci. 2015, 4(1), 296–309.
37. Benny, P., Biological Properties of L-Asparaginase. 2010, Mahatma Gandhi University, India, PhD thesis.
38. Selvam, K.,; Vishnupriya, B., Partial Purification and Cytotoxic activity of L-Asparaginase from Streptomyces acrimycini NGP. Int. J. Res. Pharm. Biomed. Sci. 2013, 4(3), 859–869.
39. Hosamani, R.,; Kaliwal, B., Isolation, Molecular Identification and Optimization of Fermentation Parameters for the Production of L-Asparaginase, an Anticancer Agent by Fusarium equiseti. Int. J. Microbiol. Res. 2011, 3(2), 108–119.
40. Kumar, K.,; Punia, S.,; Verma, N., Media Optimization for the Production of Anti-leukemic Enzyme L-Asparaginase from E. coli K-12. Ann. Biol. Res. 2012, 3(10), 4828–4837.
41. Mahajan, R.V.,; Saran, S.,; Kameswaran, K.,; Kumar, V.,; Saxena, R., Efficient Production of L-Asparaginase from Bacillus licheniformis with Low-glutaminase Activity:Optimization, Scale up and Acrylamide Degradation Studies. Bioresour. Technol. 2012, 125, 11–16.
42. Gurunathan, B.,; Sahadevan, R., Production of L-Asparaginase from Natural Substrates by Aspergillus terreus MTCC 1782:Optimization of Carbon Source and Operating Conditions. Int. J. Chem. Reactor Eng. 2011, 9, 1–15.
43. Mekala, N.K.,; Singhania, R.R.,; Sukumaran, R.K.,; Pandey, A., Cellulase Production Under Solid-state Fermentation by Trichoderma reesei RUT C30:Statistical Optimization of Process Parameters. Appl. Biochem. Biotechnol. 2008, 151(2–3), 122–131.
44. Potumarthi, R.,; Liu, J.,; Wan, H.,; Danquah, M., Surface Immobilization of Rhizopus oryzae (ATCC 96382) for Enhanced Production of Lipase Enzyme by Multiple Responses Optimization. Asia Pac. J. Chem. Eng. 2012, 7(S3), S285–S295.
45. Montgomery, D.C.,; Myers, R.H., Response Surface Methodology:Process and Product Optimization using Designed Experiments. Wiley-Interscience Publications:New York, 1995.
46. Cochran, W.,; Cox, G., Experimental Designs. Oxford, England:John Wiley & Sons, 2nd edition. 1957.
47. Baskar, G.,; Rajasekar, V.,; Renganathan, S., Modeling and Optimization of L-Asparaginase Production by Enterobacter aerogenes using Artificial Neural Network Linked Genetic Algorithm. Int. J. Chem. Eng. Appl. 2011, 2(2), 98–100.
48. Baskar, G.,; Dharmendira Kumar, M.,; Anand Prabu, A.,; Renganathan, S.,; Yoo, C., Optimization of Carbon and Nitrogen Sources for L-Asparaginase Production by Enterobacter aerogenes using Response Surface Methodology. Chem. Biochem. Eng. Q. 2009, 23(3), 393–397.
49. Singhal, B.,; Swaroop, K., Optimization of Culture Variables for the Production of L-Asparaginase from Pectobacterium carotovorum. Afr. J. Biotechnol. 2013, 12(50), 6959–6967.
50. Mahajan, R.V.,; Mihooliya, K.N.,; Saran, S.D.,; Saxena, R.K., L-Asparaginase from Bacillus sp. RKS-20:Process Optimization and Application in the Inhibition of Acrylamide Formation in Fried Foods. J. Proteins Proteomics 2014, 5(2), 125–132.
51. Kenari, S.L.D.,; Alemzadeh, I.,; Maghsodi, V., Production of L-Asparaginase from Escherichia coli ATCC 11303:Optimization by Response Surface Methodology. Food. Bioprod. Process. 2011, 89(4), 315–321.
52. Shakambari, G.,; Sumi, B.M.,; Ashokkumar, B.,; Palanivelu, P.,; Varalakshmi, P., Industrial Effluent as a Substrate for Glutaminase Free L-Asparaginase Production from Pseudomonas plecoglossicida Strain RS1; Media Optimization, Enzyme Purification and Its Characterization. RSC Adv. 2015, 5(60), 48729–48738.
53. Kumar, N.M.,; Ramasamy, R.,; Manonmani, H.K., Production and Optimization of L-Asparaginase from Cladosporium sp. using Agricultural Residues in Solid State Fermentation. Ind. Crops Prod. 2013, 43, 150–158.
54. Sanjeeviroyar, A.,; Rajendran, A.,; Muthuraj, M.,; Basha, K.M.,; Thangavelu, V., Sequential Optimization and Kinetic Modeling of L‐Asparaginase Production by Pectobacterium carotovorum in Submerged Fermentation. Asia Pac. J. Chem. Eng. 2010, 5(5), 743–755.
55. Liu, F.S.,; Zajic, J.E., L-Asparaginase Synthesis by Erwinia aroideae. Appl. Microbial. 1972, 23(3), 667–668.