Constitutive expression and optimization of nutrients for streptokinase production by pichia pastoris using statistical methods

Applied Biochemistry and Biotechnology

Volumn 158, Issue 1, 2009, Pages 25-40

  Vellanki, Ravi Nagaraj ^a   Potumarthi, Ravichandra ^b   Mangamoori, Lakshmi Narasu ^a  

^a JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY   (India)

^b INDIAN INSTITUTE OF CHEMICAL TECHNOLOGY   (India)

Author keywords

Constitutive expression; Optimization; Pichia pastoris; Placket Burman design; Response surface methodology (RSM); Streptokinase

Indexed keywords

CARBON AND NITROGEN; EXPRESSION LEVELS; FIBRIN CLOTS; MEDIUM COMPOSITION; OPTIMUM CONDITIONS; P. PASTORIS; PICHIA PASTORIS; PLACKET-BURMAN DESIGN; PLACKETT-BURMAN; PRODUCTION MEDIUM; RECOMBINANT EXPRESSION SYSTEM; RESPONSE SURFACE METHODOLOGY; STATISTICAL DESIGN; STATISTICAL EXPERIMENTAL DESIGN; VALIDATION STUDY;

CLONING; DESIGN; DEXTROSE; STATISTICS; SURFACE PROPERTIES;

OPTIMIZATION;

CARBON; GLUCOSE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; NITROGEN; PEPTONE; RECOMBINANT ENZYME; STREPTOKINASE;

ARTICLE; CLONE; ENZYME SYNTHESIS; GENE; NONHUMAN; NUTRIENT; PH; PICHIA PASTORIS; PLACKETT BURMAN DESIGN; PROCESS OPTIMIZATION; PROMOTER REGION; RESPONSE SURFACE METHOD; SKC GENE; STATISTICAL ANALYSIS; STATISTICS; TEMPERATURE; BIOSYNTHESIS; CHEMISTRY; CULTURE MEDIUM; GENE EXPRESSION; GENE VECTOR; GENETICS; METABOLISM; MOLECULAR CLONING; PICHIA;

PICHIA PASTORIS;

CARBON; CLONING, MOLECULAR; CULTURE MEDIA; GENE EXPRESSION; GENETIC VECTORS; NITROGEN; PICHIA; STREPTOKINASE;

EID: 70349582717     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-008-8315-z     Document Type: Article

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