Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids

Journal of Structural Biology

Volumn 198, Issue 1, 2017, Pages 38-42

  Snijder, Joost ^a   Borst, Andrew J ^a   Dosey, Annie ^a   Walls, Alexandra C ^a   Burrell, Anika ^a   Reddy, Vijay S ^b   Kollman, Justin M ^a   Veesler, David ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Cryo electron microscopy; Single particle; Vitrification

Indexed keywords

ETHANE;

ARTICLE; BLOTTING; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CORONAVIRIDAE; CRYOELECTRON MICROSCOPY; DENSITY; FREEZING; HUMAN ADENOVIRUS C; NONHUMAN; PARTICLE SIZE; VIRUS PARTICLE; VITRIFICATION; ECONOMICS; MACROMOLECULE; PROCEDURES; SPECIMEN HANDLING;

CRYOELECTRON MICROSCOPY; FREEZING; MACROMOLECULAR SUBSTANCES; METHODS; SPECIMEN HANDLING; VITRIFICATION;

EID: 85013932755     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2017.02.008     Document Type: Article

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