Novel applications for a noninvasive sampling method of the nasal mucosa

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 312, Issue 2, 2016, Pages L288-L296

  Rebuli, Meghan E ^a   Speen, Adam M ^a   Clapp, Phillip W ^a   Jaspers, Ilona ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

Biomarkers; Epithelial lining fluid; Innate immune status; Nasal mucosa; Storage conditions

Indexed keywords

ABSORBENT; BACTERIAL RNA; BIOLOGICAL MARKER; GAMMA INTERFERON INDUCIBLE PROTEIN 10; INTERLEUKIN 1BETA; INTERLEUKIN 6; INTERLEUKIN 8; LEUKOCYTE ELASTASE; RECOMBINANT CYTOKINE; VIRUS RNA; RECOMBINANT PROTEIN;

ABSORBENT LEUKOSORB PAPER; ADULT; ANTIBIOTIC RESISTANT BACTERIA; ARTICLE; BACTERIUM; CONTROLLED STUDY; DILUTION; ELUTION; EPITHELIAL LINING FLUID; FEMALE; FREEZING; HUMAN; INFLUENZA VIRUS; MALE; METHODOLOGY; NASAL LAVAGE; NEUTROPHIL; NON INVASIVE PROCEDURE; NORMAL HUMAN; NOSE MUCOSA; PAPER; PRIORITY JOURNAL; ROOM TEMPERATURE; SAMPLING; SEX DIFFERENCE; STORAGE TEMPERATURE; EPITHELIUM; METABOLISM; NASAL LAVAGE FLUID; PHYSIOLOGY; PROCEDURES; SPECIMEN HANDLING; YOUNG ADULT;

ADULT; EPITHELIUM; FEMALE; HUMANS; INTERLEUKIN-8; MALE; NASAL LAVAGE FLUID; NASAL MUCOSA; RECOMBINANT PROTEINS; SPECIMEN HANDLING; YOUNG ADULT;

EID: 85012040166     PISSN: 10400605     EISSN: 15221504     Source Type: Journal    
DOI: 10.1152/ajplung.00476.2016     Document Type: Article

References (37)

1. Abou Shady HM, Bakr AE, Hashad ME, Alzohairy MA. Staphylococcus aureus nasal carriage among outpatients attending primary health care centers: a comparative study of two cities in Saudi Arabia and Egypt. Braz J Infect Dis 19: 68–76, 2015. doi:10.1016/j.bjid.2014.09.005.
2. Alexis NE, Hu SC, Zeman K, Alter T, Bennett WD. Induced sputum derives from the central airways: confirmation using a radiolabeled aerosol bolus delivery technique. Am J Respir Crit Care Med 164: 1964–1970, 2001. doi:10.1164/ajrccm.164.10.2104051.
3. Atlas LT. Description of apparatus and method for obtaining nasal washings. J Lab Clin Med 32: 1016–1023, 1947.
4. Aziz N, Nishanian P, Mitsuyasu R, Detels R, Fahey JL. Variables that affect assays for plasma cytokines and soluble activation markers. Clin Diagn Lab Immunol 6: 89–95, 1999.
5. Beule AG. Physiology and pathophysiology of respiratory mucosa of the nose and the paranasal sinuses. GMS Curr Top Otorhinolaryngol Head Neck Surg 9: Doc07, 2010. doi:10.3205/cto000071.
6. Cassol S, Salas T, Gill MJ, Montpetit M, Rudnik J, Sy CT, O’Shaughnessy MV. Stability of dried blood spot specimens for detection of human immunodeficiency virus DNA by polymerase chain reaction. J Clin Microbiol 30: 3039–3042, 1992.
7. Chambers AG, Percy AJ, Yang J, Camenzind AG, Borchers CH. Multiplexed quantitation of endogenous proteins in dried blood spots by multiple reaction monitoring-mass spectrometry. Mol Cell Proteomics 12: 781–791, 2013. doi:10.1074/mcp.M112.022442.
8. Chaves-Moreno D, Wos-Oxley ML, Jáuregui R, Medina E, Oxley AP, Pieper DH. Exploring the transcriptome of Staphylococcus aureus in its natural niche. Sci Rep 6: 33174, 2016. doi:10.1038/srep33174.
9. Chawes BL, Edwards MJ, Shamji B, Walker C, Nicholson GC, Tan AJ, Folsgaard NV, Bonnelykke K, Bisgaard H, Hansel TT. A novel method for assessing unchallenged levels of mediators in nasal epithelial lining fluid. J Allergy Clin Immuno 125: 1387–1389.e3, 2010. doi:10.1016/j.jaci.2010.01.039.
10. Crimmins E, Kim JK, McCreath H, Faul J, Weir D, Seeman T. Validation of blood-based assays using dried blood spots for use in large population studies. Biodemogr Soc Biol 60: 38–48, 2014. doi:10.1080/19485565.2014.901885.
11. de Jager W, Bourcier K, Rijkers GT, Prakken BJ, Seyfert-Margolis V. Prerequisites for cytokine measurements in clinical trials with multiplex immunoassays. BMC Immunol 10: 52, 2009. doi:10.1186/1471-2172-10-52.
12. DeLeo FR, Otto M, Kreiswirth BN, Chambers HF. Communityassociated methicillin-resistant Staphylococcus aureus. Lancet 375: 1557–1568, 2010. doi:10.1016/S0140-6736(09)61999-1.
13. Duran N, Yildirim Y, Duran GG, Pasa O, Kilinc C, Yildirim I, Eryilmaz N, Bayraktar S. Virulence factors in staphylococci isolated from nasal cavities of footballers. Am J Med Sci 351: 279–285, 2016. doi:10.1016/j.amjms.2015.12.016.
14. Feneley A, Rainer M, Haider F, Narayanswamy B, Stenning J, Mordin C, Greenaway S, Clarke G. The nose as a research tool: intra-subject variability in nasal sampling. Eur Respir J 38, Suppl 55: 4071, 2011. http://erj.ersjournals.com/content/38/Suppl_55/p4071.
15. Følsgaard NV, Chawes BL, Rasmussen MA, Bischoff AL, Carson CG, Stokholm J, Pedersen L, Hansel TT, Bønnelykke K, Brix S, Bisgaard H. Neonatal cytokine profile in the airway mucosal lining fluid is skewed by maternal atopy. Am J Respir Crit Care Med 185: 275–280, 2012. doi:10.1164/rccm.201108-1471OC.
16. Grüner N, Stambouli O, Ross RS. Dried blood spots—preparing and processing for use in immunoassays and in molecular techniques. J Vis Exp 13: 2015. doi:10.3791/52619.
17. Hentschel J, Müller U, Doht F, Fischer N, Böer K, Sonnemann J, Hipler C, Hünniger K, Kurzai O, Markert UR, Mainz JG. Influences of nasal lavage collection-, processing-and storage methods on inflammatory markers—evaluation of a method for non-invasive sampling of epithelial lining fluid in cystic fibrosis and other respiratory diseases. J Immunol Methods 404: 41–51, 2014. doi:10.1016/j.jim.2013.12.003.
18. Hernandez DR, Newton DW, Ledeboer NA, Buchan B, Young C, Clark AE, Connoly J, Wolk DM. Multicenter evaluation of MRSASelect II chromogenic agar for identification of methicillin-resistant Staphylococcus aureus from wound and nasal specimens. J Clin Microbiol 54: 305–311, 2016. doi:10.1128/JCM.02410-15.
19. Horvath KM, Herbst M, Zhou H, Zhang H, Noah TL, Jaspers I. Nasal lavage natural killer cell function is suppressed in smokers after live attenuated influenza virus. Respir Res 12: 102, 2011. doi:10.1186/1465-9921-12-102.
20. Jackson D, Clements Y, Johnston S, Hansel T. P178 validation of a novel synthetic absorptive matrix (SAM) for sampling nasal mucosal lining fluid. Thorax 65, Suppl 4: A153–A153, 2010. doi:10.1136/thx.2010.151043.29.
21. Jaspers I, Ciencewicki JM, Zhang W, Brighton LE, Carson JL, Beck MA, Madden MC. Diesel exhaust enhances influenza virus infections in respiratory epithelial cells. Toxicol Sci 85: 990–1002, 2005. doi:10.1093/toxsci/kfi141.
22. Jaspers I, Zhang W, Fraser A, Samet JM, Reed W. Hydrogen peroxide has opposing effects on IKK activity and IkappaBalpha breakdown in airway epithelial cells. Am J Respir Cell Mol Biol 24: 769–777, 2001. doi:10.1165/ajrcmb.24.6.4344.
23. Kluytmans J, van Belkum A, Verbrugh H. Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clin Microbiol Rev 10: 505–520, 1997.
24. Lehmann S, Delaby C, Vialaret J, Ducos J, Hirtz C. Current and future use of “dried blood spot” analyses in clinical chemistry. Clin Chem Lab Med 51: 1897–1909, 2013. doi:10.1515/cclm-2013-0228.
25. Lipiäinen T, Peltoniemi M, Sarkhel S, Yrjönen T, Vuorela H, Urtti A, Juppo A. Formulation and stability of cytokine therapeutics. J Pharm Sci 104: 307–326, 2015. doi:10.1002/jps.24243.
26. Lü FX, Esch RE. Novel nasal secretion collection method for the analysis of allergen specific antibodies and inflammatory biomarkers. J Immunol Methods 356: 6–17, 2010. doi:10.1016/j.jim.2010.03.004.
27. Martin EM, Clapp PW, Rebuli ME, Pawlak EA, Glista-Baker E, Benowitz NL, Fry RC, Jaspers I. E-cigarette use results in suppression of immune and inflammatory-response genes in nasal epithelial cells similar to cigarette smoke. Am J Physiol Lung Cell Mol Physiol 311: L135–L144, 2016. doi:10.1152/ajplung.00170.2016.
28. Noah TL, Zhang H, Zhou H, Glista-Baker E, Müller L, Bauer RN, Meyer M, Murphy PC, Jones S, Letang B, Robinette C, Jaspers I. Effect of broccoli sprouts on nasal response to live attenuated influenza virus in smokers: a randomized, double-blind study. PLoS One 9: e98671, 2014. doi:10.1371/journal.pone.0098671.
29. Noah TL, Zhou H, Jaspers I. Alteration of the nasal responses to influenza virus by tobacco smoke. Curr Opin Allergy Clin Immunol 12: 24–31, 2012. doi:10.1097/ACI.0b013e32834ecc80.
30. Noah TL, Zhou H, Monaco J, Horvath K, Herbst M, Jaspers I. Tobacco smoke exposure and altered nasal responses to live attenuated influenza virus. Environ Health Perspect 119: 78–83, 2011. doi:10.1289/ehp.1002258.
31. Noah TL, Zhou H, Zhang H, Horvath K, Robinette C, Kesic M, Meyer M, Diaz-Sanchez D, Jaspers I. Diesel exhaust exposure and nasal response to attenuated influenza in normal and allergic volunteers. Am J Respir Crit Care Med 185: 179–185, 2012. doi:10.1164/rccm.201103-0465OC.
32. Scadding GW, Calderon MA, Bellido V, Koed GK, Nielsen N-C, Lund K, Togias A, Phippard D, Turka LA, Hansel TT, Durham SR, Wurtzen PA. Optimisation of grass pollen nasal allergen challenge for assessment of clinical and immunological outcomes. J Immunol Methods 384: 25–32, 2012. doi:10.1016/j.jim.2012.06.013.
33. Villar LM, de Oliveira JC, Cruz HM, Yoshida CF, Lampe E, Lewis-Ximenez LL. Assessment of dried blood spot samples as a simple method for detection of hepatitis B virus markers. J Med Virol 83: 1522–1529, 2011. doi:10.1002/jmv.22138.
34. Vissers M, de Groot R, Ferwerda G. Severe viral respiratory infections: are bugs bugging? Mucosal Immunol 7: 227–238, 2014. doi:10.1038/mi.2013.93.
35. Woolhouse IS, Bayley DL, Stockley RA. Effect of sputum processing with dithiothreitol on the detection of inflammatory mediators in chronic bronchitis and bronchiectasis. Thorax 57: 667–671, 2002. doi:10.1136/thorax.57.8.667.
36. Wright HL, Moots RJ, Bucknall RC, Edwards SW. Neutrophil function in inflammation and inflammatory diseases. Rheumatology (Oxford) 49: 1618–1631, 2010. doi:10.1093/rheumatology/keq045.
37. Zeilhofer HU, Schorr W. Role of interleukin-8 in neutrophil signaling. Curr Opin Hematol 7: 178–182, 2000. doi:10.1097/00062752-200005000-00009.