Simple downstream process based on detergent treatment improves yield and in vivo transduction efficacy of adeno-associated virus vectors

Molecular Therapy Methods and Clinical Development

Volumn 2, Issue , 2015, Pages 15024-

  Dias Florencio, Gabriella ^a   Precigout, Guillaume ^a   Beley, Cyriaque ^a   Buclez, Pierre Olivier ^a   Garcia, Luis ^a   Benchaouir, Rachid ^a  

^a LABORATOIRE DES SCIENCES DU CLIMAT ET DE L ENVIRONNEMENT   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ADENO ASSOCIATED VIRUS VECTOR; DETERGENT; ENDONUCLEASE; IODIXANOL; DODECYL SULFATE SODIUM; TRITON X 100;

ADULT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL VIABILITY; CONTROLLED STUDY; CYTOLYSIS; DENSITY GRADIENT; DOWNSTREAM PROCESSING; DRUG EFFICACY; EMBRYO; GENETIC TRANSFECTION; HEK293 CELL LINE; HUMAN; HUMAN CELL; IN VITRO GENE TRANSFER; IN VIVO GENE TRANSFER; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SILVER STAINING; VIRAL GENE DELIVERY SYSTEM; VIRUS PARTICLE; VIRUS PURIFICATION; ANIMAL EXPERIMENT; CONDITIONED MEDIUM; FILTRATION; FREEZE THAWING; IN VITRO STUDY; ISOPYCNIC DENSITY GRADIENT; MICROSCOPY; PHASE MICROSCOPY; QUANTITATIVE ANALYSIS; SUPERNATANT; TANGENTIAL FLOW FILTRATION; ULTRACENTRIFUGATION;

EID: 85011362038     PISSN: None     EISSN: 23290501     Source Type: Journal    
DOI: 10.1038/mtm.2015.24     Document Type: Article

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