Scalable purification of adeno-associated virus serotype 1 (AAV1) and AAV8 vectors, using dual ion-exchange adsorptive membranes

Human Gene Therapy

Volumn 20, Issue 9, 2009, Pages 1013-1021

  Okada, Takashi ^a,b   Nonaka Sarukawa, Mutsuko ^b   Uchibori, Ryosuke ^b   Kinoshita, Kazue ^a   Hayashita Kinoh, Hiromi ^a   Nitahara Kasahara, Yuko ^a   Takeda, Shin'Ichi ^a   Ozawa, Keiya ^b  

^a NATIONAL INSTITUTE OF NEUROSCIENCE   (Japan)

^b JICHI MEDICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PARVOVIRUS VECTOR; RETROVIRUS VECTOR;

ADENO ASSOCIATED VIRUS; ADENO ASSOCIATED VIRUS 1; ADENO ASSOCIATED VIRUS 8; ADSORPTION; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CONVALESCENCE; ELECTRON MICROSCOPY; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; IN VIVO STUDY; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC FOCUSING; MALE; MOUSE; NONHUMAN; RAT; SEROTYPE; VIRUS ENVELOPE; VIRUS PURIFICATION; VIRUS RECOMBINANT; VIRUS TYPING; X CHROMOSOME LINKED MUSCULAR DYSTROPHIC MOUSE;

ADSORPTION; ANIMALS; CELL LINE; CHROMATOGRAPHY, LIQUID; DEPENDOVIRUS; DOGS; GENETIC VECTORS; HUMANS; ION EXCHANGE; ISOELECTRIC FOCUSING; KIDNEY; MALE; MEMBRANES; MYOBLASTS; RATS; RATS, WISTAR; RECOMBINATION, GENETIC; SEROTYPING; TRANSFECTION;

ADENO-ASSOCIATED VIRUS;

EID: 70349129594     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.2009.006     Document Type: Article

References (16)

1. Duffy, A.M., O'Doherty, A.M., O'Brien, T., and Strappe, P.M. 2005). Purification of adenovirus and adeno-associated virus: Comparison of novel membrane-based technology to conventional techniques. Gene Ther. 12(Suppl. 1), S62-S72.
2. Gao, G., Qu, G., Burnham, M.S., Huang, J., Chirmule, N., Joshi, B., Yu, Q.C., Marsh, J.A., Conceicao, C.M., and Wilson, J.M. (2000). Purification of recombinant adeno-associated virus vectors by column chromatography and its performance in vivo. Hum. Gene Ther. 11, 2079-2091.
3. Glimm, H., Flugge, K., Mobest, D., Hofmann, V.M., Postmus, J., Henschler, R., Lange, W., Finke, J., Kiem, H.P., Schulz, G., Rosenthal, F., Mertelsmann, R., and Von Kalle, C. (1998). Efficient serum-free retroviral gene transfer into primitive human hematopoietic progenitor cells by a defined, high-titer, nonconcentrated vector-containing medium. Hum. Gene Ther. 9, 771-778.
4. Kaludov, N., Handelman, B., and Chiorini, J.A. (2002). Scalable purification of adeno-associated virus type 2, 4, or 5 using ion-exchange chromatography. Hum. Gene Ther. 13, 1235-1243.
5. Knudsen, H.L., Fahrner, R.L., Xu, Y., Norling, L.A., and Blank, G.S. (2001). Membrane ion-exchange chromatography for process-scale antibody purification. J. Chromatogr. A 907, 145-154.
6. Merten, O.W., Geny-Fiamma, C., and Douar, A.M. (2005). Current issues in adeno-associated viral vector production. Gene Ther. 12(Suppl. 1), S51-S61.
7. Okada, T., Shimazaki, K., Nomoto, T., Matsushita, T., Mizukami, H., Urabe, M., Hanazono, Y., Kume, A., Tobita, K., Ozawa, K., and Kawai, N. (2002). Adeno-associated viral vector-mediated gene therapy of ischemia-induced neuronal death. Methods Enzymol. 346, 378-393. (Pubitemid 41103149)
8. Okada, T., Caplen, N.J., Ramsey, W.J., Onodera, M., Shimazaki, K., Nomoto, T., Ajalli, R., Wildner, O., Morris, J., Kume, A., Hamada, H., Blaese, R.M., and Ozawa, K. (2004). In situ generation of pseudotyped retroviral progeny by adenovirus-mediated transduction of tumor cells enhances the killing effect of HSV-tk suicide gene therapy in vitro and in vivo. J. Gene Med. 6, 288-299.
9. Okada, T., Nomoto, T., Yoshioka, T., Nonaka-Sarukawa, M., Ito, T., Ogura, T., Iwata-Okada, M., Uchibori, R., Shimazaki, K., Mizukami, H., Kume, A., and Ozawa, K. (2005). Large-scale production of recombinant viruses using a large culture vessel with active gassing. Hum. Gene Ther. 16, 1212-1218.
10. Ory, D.S., Neugeboren, B.A., and Mulligan, R.C. (1996). A stable human-derived packaging cell line for production of high titer retrovirus=vesicular stomatitis virus G pseudotypes. Proc. Natl. Acad. Sci. U.S.A. 93, 11400-11406.
11. Phillips, M., Cormier, J., Ferrence, J., Dowd, C., Kiss, R., Lutz, H., and Carter, J. (2005). Performance of a membrane adsorber for trace impurity removal in biotechnology manufacturing. J. Chromatogr. A 1078, 74-82.
12. Sommer, J.M., Smith, P.H., Parthasarathy, S., Isaacs, J., Vijay, S., Kieran, J., Powell, S.K., McClelland, A., and Wright, J.F. (2003). Quantification of adeno-associated virus particles and empty capsids by optical density measurement. Mol. Ther. 7, 122-128.
13. Suzuki, A., Obi, K., Urabe, T., Hayakawa, H., Yamada, M., Kaneko, S., Onodera, M., Mizuno, Y., and Mochizuki, H. (2002). Feasibility of ex vivo gene therapy for neurological disorders using the new retroviral vector GCDNsap packaged in the vesicular stomatitis virus G protein. J. Neurochem. 82, 953-960.
14. Tamura, K., Tamura, M., Ikenaka, K., Yoshimatsu, T., Miyao, Y., Nanmoku, K., and Shimizu, K. (2001). Eradication of murine brain tumors by direct inoculation of concentrated high titer-recombinant retrovirus harboring the herpes simplex virus thymidine kinase gene. Gene Ther. 8, 215-222.
15. Urabe, M., Xin, K.Q., Obara, Y., Nakakura, T., Mizukami, H., Kume, A., Okuda, K., and Ozawa, K. (2006). Removal of empty capsids from type 1 adeno-associated virus vector stocks by anion-exchange chromatography potentiates trans-gene expression. Mol. Ther. 13, 823-828.
16. Yuasa, K., Sakamoto, M., Miyagoe-Suzuki, Y., Tanouchi, A., Yamamoto, H., Li, J., Chamberlain, J.S., Xiao, X., and Takeda, S. (2002). Adeno-associated virus vector-mediated gene transfer into dystrophin-deficient skeletal muscles evokes enhanced immune response against the transgene product. Gene Ther. 9, 1576-1588.