Creation of novel protein variants with CRISPR/Cas9-mediated mutagenesis: Turning a screening by-product into a discovery tool

PLoS ONE

Volumn 12, Issue 1, 2017, Pages

  Donovan, Katherine F ^a   Hegde, Mudra ^a   Sullender, Meagan ^a   Vaimberg, Emma W ^a   Johannessen, Cory M ^a   Root, David E ^a   Doench, John G ^a  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

B RAF KINASE; CASPASE 9; CRISPR ASSOCIATED PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; SELUMETINIB; VEMURAFENIB; BRAF PROTEIN, HUMAN; GUIDE RNA; HYBRID PROTEIN; INDOLE DERIVATIVE; MAP2K1 PROTEIN, HUMAN; SULFONAMIDE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DNA END JOINING REPAIR; DNA SEQUENCE; GAIN OF FUNCTION MUTATION; GENE AMPLIFICATION; HUMAN; HUMAN CELL; MUTAGENESIS; NEXT GENERATION SEQUENCING; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; PROTEIN TARGETING; RNA SEQUENCE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; VIRAL GENE DELIVERY SYSTEM; ALLELE; CHEMISTRY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRISPR CAS SYSTEM; GENE LIBRARY; GENETIC TRANSDUCTION; GENETICS; HEK293 CELL LINE; INDEL MUTATION; PHENOTYPE; PROCEDURES; PROTEIN ENGINEERING; TUMOR CELL LINE;

ALLELES; CELL LINE, TUMOR; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DNA END-JOINING REPAIR; GENE LIBRARY; HEK293 CELLS; HUMANS; INDEL MUTATION; INDOLES; MAP KINASE KINASE 1; MUTAGENESIS; PHENOTYPE; PROTEIN ENGINEERING; PROTO-ONCOGENE PROTEINS B-RAF; RECOMBINANT FUSION PROTEINS; RNA, GUIDE; SEQUENCE ALIGNMENT; SULFONAMIDES; TRANSDUCTION, GENETIC;

EID: 85010792406     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0170445     Document Type: Article

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