High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events

Optics Express

Volumn 15, Issue 24, 2007, Pages 15656-15673

  Grant, D M ^a   Mc Ginty, J ^a   Mc Ghee, E J ^a   Bunney, T D ^b   Owen, D M ^a   Talbot, C B ^a   Zhang, W ^b   Kumar, S ^a   Munro, I ^a   Lanigan, P M P ^a   Kennedy, G T ^a   Dunsby, C ^a   Magee, A I ^a   Courtney, P ^c   Katan, M ^b   Neil, M A A ^a   French, P M W ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b INSTITUTE OF CANCER RESEARCH   (United Kingdom)

^c PERKINELMER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 85010092018     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.15.015656     Document Type: Article

References (43)

1. B. N. Giepmans, S. R. Adams, M. H. Ellisman, and R. Y. Tsien, “The fluorescence toolbox for assessing protein location and function,” Science Apr 312, 217-24 (2006).
2. F. S. Wouters, P. J. Verveer, and P. I. Bastiaens, “Imaging biochemistry inside cells,” Trends Cell Biol. May 11, 203-211 (2001).
3. D. S. Lidke, P. Nagy, B. G. Barisas, R. Heintzmann, J. N. Post, K. A. Lidke, A. H. Clayton, D. J. Arndt-Jovin, and T. M. Jovin, “Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET),” Biochem. Soc. Trans. 31, 1020-1027 (2003).
4. D. S. Elson, et al, Reviews in Fluorescence 2006 (Springer, US, 2006), Chap 22.
5. rd Edition (Springer, US, 2006).
6. R. M. Clegg, O. Holub, and C. Gohlke, “Fluorescence lifetime-resolved imaging: measuring lifetimes in an image,” Methods Enzymol. 360, 509-42 (2003).
7. E. A. Jares-Erijman and T. M. Jovin, “Imaging molecular interactions in living cells by FRET microscopy,” Curr. Opin. Chem. Biol. Oct 10(5), 409-16 (2006).
8. D. M. Grant, D. S. Elson, D. Schimpf, C. Dunsby, J. Requejo-Isidro, E. Auksorius, I. Munro, M. A. A. Neil, P. M. W. French, E. Nye, G. W. Stamp, and P. Courtney “Optically sectioned fluorescence lifetime imaging using a Nipkow disc microscope and a tunable ultrafast continuum excitation source,” Opt. Lett. 30, 3353-3355 (2005).
9. E. B. van Munster, J. Goedhart, G. J. Kremers, E. M. Manders, and T. W. Gadella, “Combination of a spinning disc confocal unit with frequency-domain fluorescence lifetime imaging microscopy,” Cytometry A. Apr 71, 207-214 (2007).
10. M. J. Cole, J. Siegel, S. E. Webb, R. Jones, K. Dowling, M. J. Dayel, D. Parsons-Karavassilis, P. M. W. French, M. J. Lever, L. O. Sucharov, M. A. A. Neil, R. Juskaitis, and T. Wilson, “Time domain whole-field fluorescence lifetime imaging with optical sectioning,” J. Microsc. 203, 246-57 (2001).
11. H. C. Gerritsen, M. A. H. Asselbergs, A. V. Agronskala, and W. G. J. H. M van Sark, “Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution,” J. Microsc. 206, 218-224 (2002).
12. M. Kollner and J. Wolfrum, How many photons are necessary for fluorescence-lifetime measurements? Chem. Phys. Lett. 200, 199-204 (1992).
13. P. J. Verveer and P. I. Bastiaens, “Evaluation of global analysis algorithms for single frequency fluorescence lifetime imaging microscopy data,” J. Microsc. Jan 209, 1-7 (2003).
14. D. V. O’connor and D. Phillips: Time-Correlated Single Photon Counting (Academic Press, London, 1984).
15. W. Becker, Advanced Time Correlated Single Photon Counting Techniques, (Springer, New York 2005).
16. E. Wang, C. M. Babbey, and K. W. Dunn, “Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems,” J. Microsc. 218, 148-159 (2005).
17. M. Straub and S. W. Hell, “Fluorescence lifetime three-dimensional microscopy with picosecond precision using a multifocal multiphoton microscope,” Appl. Phys. Lett. 73, 1769-1771 (1998).
18. S. Leveque-Fort, M. P. Fontaine-Aupart, G. Roger, and P. Georges, “Fluorescence-lifetime imaging with a multifocal two-photon microscope,” Opt. Lett. 29, 2884-2886 (2004).
19. J. Requejo-Isidro, J. McGinty, I. Munro, D. S. Elson, N. P. Galletly, M. J. Lever, M. A. A. Neil, G. W. H. Stamp, P. M. W. French, P. A. Kellett, J. D. Hares, and A. K. L. Dymoke-Bradshaw, "High-speed wide-field time-gated endoscopic fluorescence- lifetime imaging," Opt. Lett. 29, 2249-2251 (2004).
20. R. K. Benninger, Y. Koc, O. Hofmann, J. Requejo-Isidro, M. A. A. Neil, P. M. W. French, and A. J. DeMello, “Quantitative 3D mapping of fluidic temperatures within microchannel networks using fluorescence lifetime imaging,” Anal. Chem. 78, 2272-2278 (2006).
21. M. A. A. Neil, R. Juskaitis, and T. Wilson, "Method of obtaining optical sectioning by using structured light in a conventional microscope," Opt. Lett. 22, 1905-1907 (1997).
22. G. McConnell, “Confocal laser scanning fluorescence microscopy with a visible continuum source,” Opt. Express 12, 2844-2850 (2004).
23. C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy J. Phys. D: Appl. Phys. 37, 3296-3303 (2004).
24. K. Simons and E. Ikonen, “Functional rafts in cell membranes,” Nature 387, 569-572 (1997)
25. A. I. Magee, N. Pirinen, J. Adler, S. N. Pagakis, and I. Parmryd, “Lipid rafts: cell surface platforms for T cell signaling,” Biol. Res. 35, 127-131 (2002).
26. G. van Meer and Q. Lisman, “Sphingolipid transport: rafts and translocators,” J. Biol. Chem. 277, 25855-25858 (2002).
27. K. Simons and R. Ehehalt, “Cholesterol, lipid rafts, and disease,” J. Clin. Invest. Sep 110, 597-603 (2002).
28. L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, “Characterization and application of a new optical probe for membrane lipid domains,” Biophys. J. 90, 2563-2575 (2006).
29. D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. M. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90, L80-L82 (2006).
30. R. R. Kellner, C. J. Baier, K. I. Willig, S. W. Hell, and F. J. Barrantes, “Nanoscale organization of nicotinic acetylcholine receptors revealed by stimulated emission depletion microscopy,” Neuroscience 144, 135-143 (2007).
31. N. Tanimura, M. Nagafuku, Y. Minaki, Y. Umeda, F. Hayashi, J. Sakakura, A. Kato, D. R. Liddicoat, M. Ogata, T. Hamaoka, and A. Kosugi, “Dynamic changes in the mobility of LAT in aggregated lipid rafts upon T cell activation,” J. Cell Biol. 160, 125-135 (2003).
32. K. Gaus, E. Gratton, E. P. Kable, A. S. Jones, I. Gelissen, L. Kritharides, and W. Jessup, “Visualizing lipid structure and raft domains in living cells with two photon microscopy,” Proc. Natl. Acad. Sci.U S A 100, 15554-15559 (2003).
33. A. Miyawaki, J. Llopis, R. Heim, J. M. McCaffery, J. A. Adams, M. Ikura, and R. Y. Tsien, “Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin,” Nature 388, 882-887 (1997).
34. T. Nakamura, K. Aoki, and M. Matsuda, “Monitoring spatio-temporal regulation of Ras and Rho GTPase with GFP-based FRET probes,” Methods 37, 146-153 (2005).
35. O. Rocks, A. Peyker, M. Kahms, P. J. Verveer, C. Koerner, M. Lumbierres, J. Kuhlmann, H. Waldmann, A. Wittinghofer, and P. I. Bastiaens, “An acylation cycle regulates localization and activity of palmitoylated Ras isoforms,” Science 307, 1746-1752 (2005).
36. A. Peyker, O. Rocks, and P. I. Bastiaenes, “Imaging activation of two Ras isoforms simultaneously in a single cell,” Chembiochem. 6, 78-85 (2005).
37. M. Augsten, R. Pusch, C. Biskup, K. Rennert, U. Wittig, K. Beyer, A. Blume, R. Wetzker, K. Friedrich, and I. Rubio, “Live-cell imaging of endogenous Ras-GTP illustrates predominant Ras activation at the plasma membrane,” EMBO Rep. 7, 46-51 (2006).
38. R. Yasuda, C. D. Harvey, H. Zhong, A. Sobczyk, L. van Aelst, and K. Svoboda, “Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging,” Nat. Neurosci. 9, 283-291 (2006).
39. D. S. Elson, et al, “Real-time time-domain fluorescence lifetime imaging including single-shot acquisition with a segmented optical image intensifier,” New J. Phys. 6, 1367-2630 (2004).
40. A. V. Agronskaia, L. Tertoolen, and H. C. Gerritsen, “High frame rate fluorescence lifetime imaging,” J. Phys. D. 36, 1655-1662 (2003).
41. R. M. Ballew and J. N. Demas, “An error analysis of the rapid lifetime determination method for the evaluation of single exponential decays,” Anal. Chem. 61, 30-33 (1989).
42. I. Munro, J. McGinty, N. Galletly, J. Requejo-Isidro, P. M. P. Lanigan, D. S. Elson, C. Dunsby, M. A. A. Neil, M. J. Lever, G.W. Stamp, and P. M. W. French “Toward the clinical application of time-domain fluorescence lifetime imaging,” J. Biomed. Opt. 10, 51403 (2005).
43. A. Esposito, C. P. Dohm, M. Bahr, and F. S. Wouters, “Unsupervised fluorescence lifetime imaging microscopy for high content and high throughput screening,” Mol. Cell Proteomics 6, 1446-54 (2007).