Time-domain whole-field fluorescence lifetime imaging with optical sectioning

Journal of Microscopy

Volumn 203, Issue 3, 2001, Pages 246-257

  Cole, M J ^a   Siegel, J ^a   Webb, S E D ^a   Jones, R ^a   Dowling, K ^a   Dayel, M J ^a   Parsons Karavassilis, D ^a   French, P M W ^a   Lever, M J ^a   Sucharov, L O D ^b   Neil, M A A ^b   Juskaitis R ^b   Wilson, T ^b  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

3D microscopy; Diode pumped laser; Fluorescence lifetime imaging; Optical sectioning; Whole field imaging

Indexed keywords

CCD CAMERAS; FLUORESCENCE IMAGING; FLUOROPHORES; LASER EXCITATION; PUMPING (LASER); SAPPHIRE; TISSUE;

3-D MICROSCOPY; DIODE PUMPED LASERS; EXCITATION SOURCES; FIELD IMAGING; FLUORESCENCE LIFETIME IMAGING; MODE-LOCKED TI: SAPPHIRE LASERS; OPTICAL SECTIONING; QUANTITATIVE IMAGING; TIME DOMAIN; WHOLE-FIELD IMAGING;

MICROSCOPES;

FLUORESCENT DYE;

ANIMAL; ARTICLE; EAR; ENERGY TRANSFER; FLUORESCENCE; FLUORESCENCE MICROSCOPY; HISTOLOGY; INSTRUMENTATION; LASER; METHODOLOGY; RAT;

ANIMALS; EAR; ENERGY TRANSFER; FLUORESCENCE; FLUORESCENT DYES; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; LASERS; MICROSCOPY, FLUORESCENCE; RATS;

EID: 10744223559     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.2001.00894.x     Document Type: Article

References (28)

1. Bambot, S.B., Rao, G., Romauld, M.M., Carter, G.M., Sipior, J., Terpetching, E. & Lakowicz, J.R. (1995) Sensing oxygen through skin using a red diode laser and fluorescence lifetimes. Biosens. Bioelect. 10, 643-652.
2. Baraga, J.J., Rava, R.P., Fitzmaurice, M., Tong, L.T., Taroni, P., Kittrell, C. & Field, M.S. (1991) Characterisation of the fluorescent morphological structures in human arterial wall using ultra-violet excited multispectrofluorimetry. Atherosclerosis, 88, 1-14.
3. Brüel, A. & Oxlund, H. (1996) Changes in biomechanical properties, composition of collagen and elastin, and advanced glycation endproducts of the rat aorta in relation to age. Atherosclerosis, 127, 155-165.
4. Carlsson, K. & Liljeborg, A. (1998) Simultaneous confocal lifetime imaging of multiple fluorophores using the intensity-modulated multiple-wavelength scanning (IMS) technique. J. Microsc. 191, 119-127.
5. Cole, M.J., Dowling, K., French, P.M.W., Jones, R., Parsons-Karavassilis, D., Siegel, J., Lever, M.J., Neil, M.A.A., Juškaitis, R., Wilson, T., Dymoke-Bradshaw, A.K.L. & Hares, J.D. (1999) Fluorescence lifetime imaging system for biomedicine and spectroscopy. Proceedings of Inter-Institute Workshop on In Vivo Optical Imaging at the NIH, pp. 18-24.
6. Cole, M.J., Siegel, J., Webb, S.E.D., Jones, R., French, P.M.W., Lever, M.J., Sucharov, L.O., Neil, M.A.A., Juškaitis, R. & Wilson, T. (2000) Whole-field optically sectioned fluorescence lifetime Imaging. Opt. Lett. 25, 1361-1363.
7. Cubeddu, R., Pifferi, A., Taroni, P., Torricelli, A., Valentini, G., Rinaldi, F. & Sorbellini, E. (1999) Fluorescence lifetime imaging: an application to the detection of skin tumors. IEEE J. Sel. Top. Quant. Elect. 5, 923-929.
8. Dowling, K., Dayel, M.J., Lever, M.J., French, P.M.W., Hares, J.D. & Dymoke-Bradshaw, A.K.L. (1998) Fluorescence lifetime imaging with picosecond resolution for biomedical applications. Opt. Lett. 23, 810-812.
9. Gadella, T.W.J., Jovin, T.M. & Clegg, R.M. (1993) Fluorescence lifetime imaging microscopy (FLIM) - spatial resolution of structures on the nanosecond timescale. Biophys. Chem. 48, 221-239.
10. 6 regenerative amplifiers. Opt. Lett. 20, 160-162.
11. Jones, R., Dowling, K., Cole, M.J., Parsons-Karavassilis, D., Lever, M.J., French, P.M.W., Hares, J.D. & Dymoke-Bradshaw, A.K.L. (1999) Fluorescence lifetime imaging using a diode-pumped all-solid-state laser system. Elect. Lett. 35, 256-257.
12. Lakowicz, J.R., Szmacinski, H. & Johnson, M.L. (1992) Calcium imaging using fluorescence lifetimes and long-wavelength probes. J. Fluorescence, 2, 47-62.
13. Mellish, R., Barry, N.P., Hyde, S.C.W., Jones, R., French, P.M.W., Taylor, J.R., Van der Poel, C.J. & Valster, A. (1995) Diode-pumped Cr: LiSAF all-solid-state femtosecond oscillator and regenerative amplifier. Opt. Lett. 20, 2312-2314.
14. Neil, M.A.A., Juškaitis, R. & Wilson, T. (1997) Method of obtaining optical sectioning by using structured light in a conventional microscope. Opt. Lett. 22, 1905-1907.
15. Neil, M.A.A., Squire, A., Juškaitis, R., Bastiaens, P.I.H. & Wilson, T. (2000) Wide-field optically sectioning fluorescence microscopy with laser illumination. J. Microsc. 197, 1-4.
16. Press, W.H., Flannery, B.P., Teukolky, S.A. & Vetterling, W.T. (1990) Numerical Recipes in C - the Art of Scientific Computing. Cambridge University Press, Cambridge.
17. Sanders, R., Draaijer, A., Gerritsen, H.C., Houpt, P.M. & Levine, Y.K. (1995) Quantitative pH imaging in cells using confocal fluorescence lifetime imaging microscopy. Anal Biochem. 227, 302-308.
18. Schneider, P.C. & Clegg, R.M. (1997) Rapid acquisition analysis and display of fluorescence lifetime-resolved images for real-time applications. Rev. Sci. Inst. 68, 4107-4119.
19. Scully, A.D., MacRobert, A.J., Botchway, S., O'Neill, P., Parker, A.W., Ostler, R.B. & Phillips, D. (1996) Development of a laser-based fluorescence microscope with subnanosecond time resolution. J. Fluorescence, 6, 119-125.
20. Squire, A. & Bastiaens, P.I.H. (1999) Three dimensional image restoration in fluorescence lifetime imaging microscopy. J. Microsc. 193, 36-49.
21. Straub, M. & Hell, S.W. (1998) Fluorescence lifetime three-dimensional microscopy with picosecond precision using a multifocal multiphoton microscope. Appl. Phys. Lett. 73, 1769-1771.
22. Systma, J., Vroom, J.M., De Grauw, C.J. & Gerritsen, H.C. (1998) Time-gated fluorescence lifetime imaging and microvolume spectroscopy using two-photon excitation. J. Microsc. 191, 39-51.
23. Szmacinski, H. & Lakowicz, J.R. (1993) Optical measurements of pH using fluorescence lifetimes and phase-modulation fluorometry. Anal. Chem. 65, 1668-1674.
24. Taylor, J.R., Adams, M.C. & Sibbett, W. (1980) Investigation of viscosity dependent fluorescence lifetime using a synchronously operated picosecond streak camera. Appl. Phys. 21, 13-17.
25. Tsien, R.Y. & Poenie, M. (1986) Fluorescence ratio imaging: a new window into intracellular ionic signalling. Trends Biochem. Sci. 11, 450-455.
26. Valentini, G., D'Andrea, C., Comelli, D., Pifferi, A., Taroni, P., Torricelli, A., Cubeddu, R., Battaglia, C., Consolandi, C., Salani, G., Rossi-Bernardi, L. & De Bellis, G. (2000) Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity. Opt. Lett. 25, 1648.
27. Weber, G. (1981) Resolution of fluorescence lifetimes in a heterogeneous system by phase and modulation measurements. J. Phys. Chem. 85, 949-953.
28. Zeng, H., MacAulay, C., Palcic, B. & McLean, D.I. (1993) A computerised autofluorescence and diffuse reflectance spectra-analyser system for in vivo skin studies. Phys. Bio. Med. 38, 231-240.