High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery

Microbial Cell Factories

Volumn 16, Issue 1, 2017, Pages

  Turchetto, Jeremy ^a   Sequeira, Ana Filipa ^b,c   Ramond, Laurie ^a   Peysson, Fanny ^a   Brás, Joana L A ^c   Saez, Natalie J ^a,d   Duhoo, Yoan ^a   Blémont, Marilyne ^a   Guerreiro, Catarina I P D ^c   Quinton, Loic ^e   Pauw, Edwin ^e   Gilles, Nicolas ^f   Darbon, Hervé ^a   Fontes, Carlos M G A ^b,c   Vincentelli, Renaud ^a  

^a CNRS   (France)

^b FACULDADE DE MEDICINA VETERINÁRIA   (Portugal)

^c NZYTech Genes and Enzymes   (Portugal)

^d UNIVERSITY OF QUEENSLAND   (Australia)

^e UNIVERSITY OF LIÈGE   (Belgium)

^f SERVICE DE CHIMIE BIOORGANIQUE ET DE MARQUAGE   (France)

Author keywords

Disulphide bonds; Drug discovery library; Escherichia coli (E. coli); High throughput production; Periplasm; Venom peptides

Indexed keywords

DISULPHIDE RETICULATED PEPTIDE; HYBRID PROTEIN; PEPTIDE; PROTEIN TAG; UNCLASSIFIED DRUG; VENOM; DISULFIDE; ESCHERICHIA COLI PROTEIN; PEPTIDE LIBRARY; PROTEINASE; RECOMBINANT PROTEIN; TEV PROTEASE;

ARTICLE; CODON; DISULFIDE BOND; DRUG MANUFACTURE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE LIBRARY; GENE SYNTHESIS; HIGH THROUGHPUT SCREENING; NONHUMAN; PEPTIDE SYNTHESIS; PERIPLASM; PLASMID; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; ANIMAL; CHEMISTRY; DRUG DEVELOPMENT; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; OXIDATION REDUCTION REACTION; PEPTIDE LIBRARY; PROCEDURES;

ANIMALS; DISULFIDES; DRUG DISCOVERY; ENDOPEPTIDASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HIGH-THROUGHPUT SCREENING ASSAYS; OXIDATION-REDUCTION; PEPTIDE LIBRARY; PEPTIDES; PERIPLASM; RECOMBINANT PROTEINS; VENOMS;

EID: 85009985911     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0617-1     Document Type: Article

References (32)

1. Escoubas P, King GF. Venomics as a drug discovery platform. Expert Rev Proteom. 2009;6:221-4.
2. Calvete JJ, Sanz L, Angulo Y, Lomonte B, Gutiérrez JM. Venoms, venomics, antivenomics. FEBS Lett. 2009;583:1736-43.
3. King GF. Venoms as a platform for human drugs: translating toxins into therapeutics. Expert Opin Biol Ther. 2011;11:1469-84.
4. Lewis RJ, Garcia ML. Therapeutic potential of venom peptides. Nat Rev Drug Discov. 2003;2:790-802.
5. Terrat Y, Sunagar K, Fry BG, Jackson TNW, Scheib H, Fourmy R, et al. Atractaspis aterrima toxins: the first insight into the molecular evolution of venom in side-stabbers. Toxins (Basel). 2013;5:1948-64.
6. Dutertre S, Jin A, Kaas Q, Jones A, Alewood PF, Lewis RJ. Deep venomicsreveals the mechanism for expanded peptide diversity in cone snail venom. Mol Cell Proteom. 2013;12:312-29.
7. Brinkman DL, Aziz A, Loukas A, Potriquet J, Seymour J, Mulvenna J. Venom proteome of the box jellyfish chironex fleckeri. Plos ONE. 2012;7:e47866.
8. King GF, Gentz MC, Escoubas P, Nicholson GM. A rational nomenclature for naming peptide toxins from spiders and other venomous animals. Toxicon. 2008;52:264-76.
9. Lavergne V, Alewood PF, Mobli M, King GF. The structural universe of disulfide-rich venom peptides. RSC Drug Discov. 2015;37:79.
10. de Marco A. Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli. Microb Cell Fact. 2009;8:26.
11. Berkmen M. Production of disulfide-bonded proteins in Escherichia coli. Protein Expr Purif. 2012;12:249-58.
12. Nozach H, Fruchart-Gaillard C, Fenaille F, Beau F, Ramos OHP, Douzi B, et al. High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli. Microb Cell Fact. 2013;12:37.
13. Sequeira AF, Turchetto J, Saez NJ, Peysson F, Ramond L, Duhoo Y, et al. Gene design, fusion technology and TEV cleavage site influence the expression of disulphide-rich venom peptides in Escherichia coli. Microb Cell Fact. 2017;16. doi: 10.1186/s12934-016-0618-0.
14. Saez NJ, Nozach H, Blemont M, Vincentelli R. High throughput quantitative expression screening and purification applied to recombinant disulfide-rich venom proteins produced in E. coli. Jove-J Vis Exp. 2014;89:1-15.
15. Saez NJ, Vincentelli R. High-throughput expression screening and purification of recombinant proteins in E. coli. Methods Mol Biol (Clifton, NJ). 2014;1091:33-53.
16. Van Den Berg S, Löfdahl PÅ, Härd T, Berglund H. Improved solubility of TEV protease by directed evolution. J Biotechnol. 2006;121:291-8.
17. LeProust EM, Peck BJ, Spirin K, McCuen HB, Moore B, Namsaraev E, et al. Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process. Nucleic Acids Res. 2010;38:2522-40.
18. Quinton L, Gilles N, De Pauw E. TxXIIIA, an atypical homodimeric conotoxin found in the Conus textile venom. J Proteom. 2009;72:219-26.
19. Karbat I, Turkov M, Cohen L, Kahn R, Gordon D, Gurevitz M, et al. X-ray structure and mutagenesis of the scorpion depressant toxin LqhIT2 reveals key determinants crucial for activity and anti-insect selectivity. J Mol Biol. 2007;366:586-601.
20. Vetter I, Davis JL, Rash LD, Anangi R, Mobli M, Alewood PF, et al. Venomics: a new paradigm for natural products-based drug discovery. Amino Acids. 2011;40:15-28.
21. Craik DJ, Fairlie DP, Liras S, Price D. The future of peptide-based drugs. Chem Biol Drug Des. 2013;81:136-47.
22. Takacs Z, York N. Animal venoms in medicine. 3rd ed. Encycl Toxicol; 2014.
23. Dutertre S, Undheim EAB, Pineda SS, Jin A-H, Lavergne V, Fry BG, et al. Venoms-based drug discovery: proteomic and transcriptomic approaches. RSC Drug Discov. 2015;11:80-96.
24. Harvey AL. Toxins and drug discovery. Toxicon. 2014;92:193-200.
25. Escoubas P, Quinton L, Nicholson GM. Venomics: unravelling the complexity of animal venoms with mass spectrometry. J Mass Spectrom. 2008;43:279-95.
26. Saaem I, Ma S, Quan J, Tian J. Error correction of microchip synthesized genes using Surveyor nuclease. Nucleic Acids Res. 2012;40:1-8.
27. Ma S, Saaem I, Tian J. Error correction in gene synthesis technology. Trends Biotechnol. 2012;30:147-54.
28. Schwartz JJ, Lee C, Shendure J. Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules. Nat Methods. 2012;9:913-5.
29. Wan W, Li L, Xu Q, Wang Z, Yao Y, Wang R, et al. Error removal in microchip-synthesized DNA using immobilized MutS. Nucleic Acids Res. 2014;42:e102.
30. Xiong A-S, Peng R-H, Zhuang J, Gao F, Li Y, Cheng Z-M, et al. Chemical gene synthesis: strategies, softwares, error corrections, and applications. FEMS Microbiol Rev. 2008;32:522-40.
31. Fuhrmann M, Oertel W, Berthold P, Hegemann P. Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage. Nucleic Acids Res. 2005;33:e58.
32. Carr PA, Church G. Genome engineering. Nat Biotechnol. 2009;27:1151-62.