An on-chip imaging droplet-sorting system: A real-time shape recognition method to screen target cells in droplets with single cell resolution

Scientific Reports

Volumn 7, Issue , 2017, Pages

  Girault, Mathias ^a   Kim, Hyonchol ^b   Arakawa, Hisayuki ^c   Matsuura, Kenji ^d,e   Odaka, Masao ^d,e   Hattori, Akihiro ^d,e   Terazono, Hideyuki ^d,e   Yasuda, Kenji ^f  

^a CENTRE DE RECHERCHE PAUL PASCAL   (France)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY   (Japan)

^d Waseda University   (Singapore)

^e Waseda University ^*  (Japan)

^f WASEDA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SELECTION; DUNALIELLA TERTIOLECTA; IMAGING; NONHUMAN; PHAEODACTYLUM TRICORNUTUM; PLANKTON; RECOGNITION; STAINING; TARGET CELL; CELL SEPARATION; CELL SHAPE; CHLAMYDOMONADALES; CYTOLOGY; DEVICES; DIATOM; FLUORESCENCE IMAGING; ISOLATION AND PURIFICATION; MICROFLUIDICS; PROCEDURES;

CELL SEPARATION; CELL SHAPE; DIATOMS; MICROFLUIDICS; OPTICAL IMAGING; VOLVOCIDA;

EID: 85008674759     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep40072     Document Type: Article

References (60)

1. Shi, W., Qin, J. Ye, N. & Lin, B. Droplet-based microfluidic system for individual Caenorhabditis elegans assay. Lab Chip 8, 1432-1435 (2008).
2. Moon, S., Ceyhan, E., Gurkan, U. A. & Demirci, U. Statistical modeling of single target cell encapsulation. Plos One 6, e21580, doi: 10.1371/journal.pone.0021580 (2011).
3. He, M. Y. et al. Selective encapsulation of single cells and subcellular organelles into picoliter-and femtoliter-volume droplets. Anal. Chem. 77, 1539-1544 (2005).
4. Chabert, M. & Viovy, J.-L. Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells. Proc. Natl. Acad. Sci. USA 105, 3191-3196 (2008).
5. Edd, J. F. et al. Controlled encapsulation of single-cells into monodisperse picolitre drops. Lab Chip. 8, 1262-1264 (2008).
6. Kemna, E. W. M. et al. High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel. Lab Chip 12, 2881-2887 (2012).
7. Jing, T. et al. High throughput single cancer cell encapsulation and self sorting for protease assay by using jetting microfluidics, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Freiburg, Germany, October 27-31 (2013).
8. Hatch A. C., Patel, A., Beer, N. R. & Lee, A. P. Passive droplet sorting using viscoelastic flow focusing. Lab Chip 13, 1308-1315 (2013).
9. Baret, J.-C. et al. Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. Lab Chip 9, 1850-1858 (2009).
10. Mazutis, L. et al. Single-cell analysis and sorting using droplet-based microfluidics. Nat. Protocols 8, 870-891 (2013).
11. Sciambi, A. & Abate, A. R. Accurate microfluidic sorting of droplets at 30 kHz. Lab Chip 15, 47-51 (2015).
12. Agresti, J. J. et al. Ultrahigh-throughput screening in drop-based microfluidics for directed evolution. Proc. Natl. Acad. Sci. USA 107, 4004-4009 (2010).
13. Debs, B. El., Utharala, R., Balyasnikova, I. V., Griffiths, A. D. & Merten, C. A. Functional single-cell hybridoma screening using droplet-based microfluidics. Proc. Natl. Acad. Sci. USA 109, 11570-11575 (2012).
14. Abate, A. R., Agresti, J. J. & Weitz, D. A. Microfluidic sorting with high-speed single-layer membrane valves. Appl. Phys. Lett. 96, 203509 (2010).
15. Cao, Z. et al. Droplet sorting based on the number of encapsulated particles using a solenoid valve. Lab Chip 13, 171-178 (2013).
16. Wu, L., Chen, P., Dong, Y., Feng, X. & Liu, B.-F. Encapsulation of single cells on a microfluidic device integrating droplet generation with fluorescence-activated droplet sorting. Biomed. Microdevices 15, 553-560 (2013).
17. Ding, X. et al. Standing surface acoustic wave (SSAW) based multichannel cell sorting. Lab Chip 12, 4228-4231 (2012).
18. Lee, C. et al. Microfluidic droplet sorting with a high frequency ultrasound beam. Lab Chip 12, 2736-2742 (2012).
19. Yasuda, K. et al. Non-destructive on-chip imaging flow cell-sorting system for on-chip cellomics. Microfluid. Nanofluid. 14, 907-931 (2013).
20. Zang, E. et al. Real-time image processing for label-free enrichment of Actinobacteria cultivated in picolitre droplets. Lab Chip 13, 3707-3713 (2013).
21. Kim, H. et al. Development of on-chip multiimaging flow cytometry for identification of imaging biomarkers of clustered circulating tumor cells. Plos One 9, e104372 (2014).
22. Hayashi, M. et al. Fully automated on-chip imaging flow cytometry system with disposable contamination-free plastic re-cultivation chip. Int. J. Mol. Sci. 12, 3618-3634 (2011).
23. Anna, S. L., Bontoux, N. & Stone, H. A. Formation of dispersions using 'flow focusing' in microchannels. Appl. Phys. Lett. 82, 364-366 (2003).
24. Fu, T., Wu, Y., Ma, Y. & Li, H. L. Droplet formation and breakup dynamics in microfluidic flow-focusing devices: From dripping to jetting. Chem. Eng. Sci. 84, 207-217 (2012).
25. Zhao, Y., Chen, G. & Yuan, Q. Liquid-Liquid two-phase flow patterns in a rectangular microchannel. AlChE J. 52, 4052-4060 (2006).
26. Salim, A., Fourar, M., Pironon J. & Sausse, J. Oil-water two phase flow in microchannels: flow patterns and pressure drop measurements. Can. J. Chem. Eng. 86, 978-988 (2008).
27. Eastburn, D. J., Sciambi, A. & Abate, A. Ultrahigh-throughput Mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic drops. Anal. Chem. 85, 8016-8021 (2013).
28. Sciambi, A. & Abate, A. R. Generating electric fields in PDMS microfluidic devices with salt water electrodes. Lab Chip 14, 2605-2609 (2014).
29. Girault, M. et al. Algorithm for the precise detection of single and cluster cells in microfluidic applications. Cytom. Part A. 89, 731-741 (2016).
30. Girault, M. et al. Particle recognition in microfluidic applications using a template matching algorithm. JPN. J. Appl. Phys. 55, doi: 10.7567/JJAP.55.06GN05 (2016).
31. Davies, D. Cell Sorting by Flow Cytometry. In: Macey, M. G., Editor. Flow Cytometry: Principles and Applications (Totowa, NJ: Humana Press Inc. 257-276 (2007).
32. Quraishi, F. O. & Spencer, C. P. Studies on the responses of marine phytoplankton to light fields of varying intensity. In "Fourth European Marine Biology Symposium" (Ed: Crisp, D. J.) Cambridge University Press 393-408, Fig 2 (1971).
33. Nelson, D. M., D'Elia, C. F. & Guillard, R. R. L. Growth and competition of the marine diatoms Phaeodactylum tricomutum and Thalassiosira pseudonana. II. Light limitation. Mar. Biol. 50, 313-318 (1979).
34. Johnston, A. M. & Raven, J. A. Effect of aeration rates on growth rates and natural abundance 13C/12C ratio of Phaeodactylum tricornutum. Mar. Ecol. Prog. Ser. 7, 295-300 (1992).
35. Torres, A. J., Hill, A. S. & Love, J. C. Nanowell-based immunoassay so for measuring single-cell secretion: Characterization of transport and surface binding. Anal. Chem. 86, 11562-11569 (2014).
36. Irie, K., Fujitani, H. & Tsuneda, S. Physical enrichment of uncultured Accumulibacter and Nitrospira from activated sludge by unlabeled cell sorting technique. J. Biosci. Bioeng. 122, 475-481, doi: 10.1016/j.jbiosc.2016.03.020 (2016).
37. Riba, J., Gleichman, T., Zimmermann, S., Zengerle, R. & Koltay, P. Label-free isolation and deposition of single bacterial cells from heterogeneous samples for clonal culturing. Sci. Rep. 6, 32837 (2016).
38. Jagannadh, V. K., Gopakumar, G., Subrahmanyam, G. R. K. S. & Gorthi, S. S. Microfluidic microscopy-assisted label-free approach for cancer screening: automated microfluidic cytology for cancer screening. Med. Biol. Eng. Comput., doi: 10.1007/s11517-016-1549-y (2016).
39. Barber, P. R. et al. An automated colony counter utilising a compact Hough transform. In "Proceedings of Medical Image Understanding and Analysis (MIUA2000)" (eds: Arridge, S. & Todd-Pokropek, A.) UCL, London 41-44 (2000).
40. Hayashi, H. & Saitoh S. Rotation invariant image matching by interpolated eight-direction block matching. Electr. Eng. Jpn. 154, 606-612 (2006).
41. Smereka, M. & Dul, I. Circular object detection using a modified Hough transform, Int. J. Appl. Math. Comput. Sci. 18, 85-91 (2008).
42. Terazono, H., Hayashi, M., Kim, H., Hattori, A. & Yasuda, K. Cell-sorting system with on chip imaging for label-free shape-based selection of cells. Jpn. J. Appl. Phys. 51, doi: 10.1143/JJAP.51.06FK08 (2012).
43. Chaddad, A. & Tanougast, C. Real-time abnormal cell detection using a deformable snake model. Health Technol. 5, 179-187 (2015).
44. Takahashi, K., Hattori, A., Suzuki, I., Ichiki, T. & Yasuda, K. Non-destructive on-chip cell sorting system with real-time microscopic image processing. J. Nanobiotechnology 2, 5 (2004).
45. Shields, C. W., Reyes, C. D. & Lopez, G. P. Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation. Lab Chip 15, 1230-1249 (2015).
46. Niu, X., Zhang, M., Peng, S., Wen, W. & Sheng, P. Real-time detection, control, and sorting of microfluidic droplets. Biomicrofluidics. 1, doi: 10.1063/1.2795392 (2007).
47. Heo, Y. S. et al. Characterization and resolution of evaporation-mediated osmolality shifts that constrain microfluidic cell culture in poly(dimethylsiloxane) devices. Anal. Chem. 79, 1126-1134 (2007).
48. Schmitz, C. H., Rowat, A. C., Köster, S. & Weitz, D. A. Dropspots: a picoliter array in a microfluidic device. Lab Chip 7, 44-49 (2009).
49. Rowat, A. C., Bird, J. C., Agresti, J. J., Rando, O. J. & Weitz, D. A. Tracking lineages of single cells in lines using a microfluidic device. Proc. Natl. Acad. Sci. USA 106, 18149-18154 (2009).
50. Faley, S. L. et al. Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells. Lab Chip 9, 2659-2664 (2009).
51. Nilsson, J., Evander, M., Hammarström, B. & Laurell, T. Review of cell and particle trapping in microfluidic systems. Anal. Chim. Acta 649, 141-157 (2009).
52. Tanyeri, M., Johnson-Chavarria, E. M. & Schroeder, C. M. Hydrodynamic trap for single particles and cell. Appl. Phys. Lett. 96, 224101 (2010).
53. Amelia, A. K. et al. A Microfluidic Device for Hydrodynamic Trapping and Manipulation Platform of a Single Biological Cell. Appl. Sci. 6, 40, doi: 10.3390/app6020040 (2016).
54. Lutz, R. B., Chen J. & Schwartz, D. T. Hydrodynamic Tweezers: 1. Noncontact Trapping of Single Cells Using Steady Streaming Microeddies. Anal. Chem. 78, 5429-5435 (2006).
55. Frimat, J.-P. et al. A microfluidic array with cellular valving for single cell co-culture. Lab Chip 11, 231-237 (2011).
56. Sarioglu, A. F. et al. A microfluidic device for label-free, physical capture of circulating tumor cell-clusters. Nat. Methods 12, 685-691 (2015).
57. Jackson, J. M. & Lenz, P. H. Predator-prey interactions in the plankton: larval fish feeding on evasive copepods. Sci. Rep. 6, 33585 (2016).
58. Probst, C., Grünberger, A., Wiechert, W. & Kohlheyer, D. Polydimethylsiloxane (PDMS) sub-micron traps for single-cell analysis of bacteria. Micromachines 4, 357-369 (2013).
59. Duffy, D. C., McDonald, J. C., Schueller, O. J. A. & Whitesides, G. M. Rapid prototyping of microfluidic system in poly(dimethylsiloxane). Anal. Chem. 70, 4974-4984 (1998).
60. Guillard, R. R. L. Culture of phytoplankton for feeding marine invertebrates. In "Culture of Marine Invertebrate Animals" (eds: Smith, W. L. & Chanley, M. H.) Plenum Press, New York, USA 26-60 (1975).