Unveiling the Pathogenic Molecular Mechanisms of the Most Common Variant (p.K329E) in Medium-Chain Acyl-CoA Dehydrogenase Deficiency by in Vitro and in Silico Approaches

Biochemistry

Volumn 55, Issue 51, 2016, Pages 7086-7098

  Bonito, Cátia A ^a,b,c   Nunes, Joana ^a,b   Leandro, João ^a,b   Louro, Filipa ^a,b   Leandro, Paula ^a,b   Ventura, Fátima V ^a,b   Guedes, Rita C ^b  

^a UNIVERSITY OF LISBON   (Portugal)

^b RESEARCH INSTITUTE FOR MEDICINES IMED ULISBOA   (Portugal)

^c UNIVERSITY OF PORTO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BINDING ENERGY; BINS; CHAINS; ENZYME ACTIVITY; FATTY ACIDS; MOLECULAR DYNAMICS; MONOMERS; OLIGOMERS; PROTEINS;

BINDING AFFINITIES; CONFORMATIONAL FLEXIBILITY; ENZYMATIC ACTIVITIES; MEDIUM-CHAIN ACYL-COA DEHYDROGENASE; MOLECULAR MECHANISM; PRECURSOR PROTEINS; RESIDUAL ACTIVITY; STRUCTURAL INSTABILITY;

DIMERS;

MEDIUM CHAIN ACYL COENZYME A DEHYDROGENASE; ACYL COENZYME A DEHYDROGENASE; GLUTAMIC ACID; LYSINE; PROTEIN BINDING;

ARTICLE; COMPUTER MODEL; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; EXPRESSION VECTOR; GENE MUTATION; IN VITRO STUDY; MEDIUM CHAIN ACYL COENZYME A DEHYDROGENASE DEFICIENCY; MICHAELIS MENTEN KINETICS; MOLECULAR DYNAMICS; MOLECULAR PATHOLOGY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PARTICLE SIZE; PHOTON CORRELATION SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; PROTON TRANSPORT; REACTION TIME; REAL TIME POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; SIZE EXCLUSION CHROMATOGRAPHY; TRANSITION TEMPERATURE; BIOCATALYSIS; CHEMISTRY; COMPUTER SIMULATION; DEFICIENCY; DISORDERS OF LIPID AND LIPOPROTEIN METABOLISM; ENZYMOLOGY; GENETICS; HUMAN; KINETICS; MISSENSE MUTATION; MOLECULAR MODEL; MOTION; PRINCIPAL COMPONENT ANALYSIS; PROTEIN DOMAIN; PROTEIN MULTIMERIZATION; TEMPERATURE;

ACYL-COA DEHYDROGENASE; BIOCATALYSIS; COMPUTER SIMULATION; ENZYME STABILITY; GLUTAMIC ACID; HUMANS; KINETICS; LIPID METABOLISM, INBORN ERRORS; LYSINE; MODELS, MOLECULAR; MOTION; MUTATION, MISSENSE; PRINCIPAL COMPONENT ANALYSIS; PROTEIN BINDING; PROTEIN DOMAINS; PROTEIN MULTIMERIZATION; TEMPERATURE;

EID: 85008440769     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.6b00759     Document Type: Article

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