Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9

Biosensors and Bioelectronics

Volumn 91, Issue , 2017, Pages 175-182

  Attayek, Peter J ^a   Waugh, Jennifer P ^b   Hunsucker, Sally A ^c   Grayeski, Philip J ^b   Sims, Christopher E ^b,d   Armistead, Paul M ^b,c   Allbritton, Nancy L ^a,c,d  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF NORTH CAROLINA   (United States)

^d University of North Carolina at Chapel Hill   (United States)

Author keywords

Cell array; Cell sorting; CRISPR Cas9; Cytometry; Microraft

Indexed keywords

AUTOMATION; CELLS; EFFICIENCY; GENE EXPRESSION; GENES; HARDWARE;

CELL ARRAY; CELL SORTING; CRISPR-CAS9; CYTOMETRY; MICRORAFT;

CYTOLOGY;

CELL SELECTION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CYTOMETRY; GENE EXPRESSION; GENE MUTATION; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; SOFTWARE; TIME LAPSE IMAGING; CRISPR CAS SYSTEM; DEVICES; EQUIPMENT DESIGN; GENETIC PROCEDURES; GENETIC TRANSFECTION; GENETICS; IMAGE PROCESSING; K-562 CELL LINE; LEUKEMIA; MUTATION; PROCEDURES; REPORTER GENE; TISSUE MICROARRAY;

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; SPLICING FACTOR U2AF; U2AF1 PROTEIN, HUMAN;

BIOSENSING TECHNIQUES; CRISPR-CAS SYSTEMS; EQUIPMENT DESIGN; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; K562 CELLS; LEUKEMIA; MUTATION; SPLICING FACTOR U2AF; TISSUE ARRAY ANALYSIS; TRANSFECTION;

EID: 85006802609     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2016.12.019     Document Type: Article

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