A novel method for immobilization of proteins via entrapment of magnetic nanoparticles through epoxy cross-linking

Analytical Biochemistry

Volumn 519, Issue , 2017, Pages 42-50

  Iype, Tessy ^a   Thomas, Jaiby ^a   Mohan, Sangeetha ^b   Johnson, Kochurani K ^a   George, Ligi E ^b   Ambattu, Lizebona A ^b   Bhati, Aniruddha ^a   Ailsworth, Kristen ^c   Menon, Bindu ^c   Rayabandla, Sunayana M ^d   Jesudasan, Rachel A ^d   Santhosh, Sam ^a,b   Ramchand, Chaniyilparampu N ^a  

^a MagGenome Technologies Pvt Ltd   (India)

^b SCIGENOM LABS PVT LTD   (India)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^d CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

Entrapment; Epichlorohydrin; Epoxy cross linking; Immobilization; Magnetic nanoparticles

Indexed keywords

EPICHLOROHYDRIN; ESTERASE; HORSERADISH PEROXIDASE; IMMOBILIZED ENZYME; IMMOBILIZED PROTEIN; IMMUNOGLOBULIN G ANTIBODY; PROTEIN A; ULTRASMALL SUPERPARAMAGNETIC IRON OXIDE; CROSS LINKING REAGENT; MAGNETITE NANOPARTICLE;

ADULT; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN BINDING; ARTICLE; CROSS LINKING; ENZYME IMMOBILIZATION; ENZYME STABILITY; FEMALE; HUMAN; IMMUNOPRECIPITATION; MAGNETIC SEPARATION; MALE; MOUSE; NONHUMAN; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN IMMOBILIZATION; PROTEIN PURIFICATION; RAT; SURFACE AREA; CHEMISTRY; IMMUNOASSAY; KINETICS; METABOLISM; PH; TEMPERATURE;

CROSS-LINKING REAGENTS; ENZYMES, IMMOBILIZED; EPICHLOROHYDRIN; ESTERASES; HORSERADISH PEROXIDASE; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOASSAY; KINETICS; MAGNETITE NANOPARTICLES; TEMPERATURE;

EID: 85006013237     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2016.12.007     Document Type: Article

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