Somatic cell nuclear transfer followed by CRIPSR/Cas9 microinjection results in highly efficient genome editing in cloned pigs

International Journal of Molecular Sciences

Volumn 17, Issue 12, 2016, Pages

  Sheets, Timothy P ^a,b   Park, Chi Hun ^a,b   Park, Ki Eun ^a,b,c   Powell, Anne ^b   Donovan, David M ^b   Telugu, Bhanu P ^a,b,c  

^a UNIVERSITY OF MARYLAND   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

^c Renovate Biosciences Inc   (United States)

Author keywords

CRISPR Cas; Knockout; Microinjection; Pig; SCNT

Indexed keywords

CRISPR ASSOCIATED PROTEIN; GROWTH FACTOR RECEPTOR BOUND PROTEIN 10; RIBONUCLEOPROTEIN; GUIDE RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BLASTOCYST; BODY MASS; CELL NUCLEUS TRANSPLANTATION; CONTROLLED STUDY; GENE FREQUENCY; GENE MUTATION; GENE OVEREXPRESSION; GENOTYPE PHENOTYPE CORRELATION; GENOTYPING TECHNIQUE; INCUBATION; MEDICAL RESEARCH; MICROINJECTION; NONHUMAN; OBESITY; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SOMATIC CELL GENETICS; ANIMAL; CLONING; CRISPR CAS SYSTEM; GENE EDITING; GENETICS; GENOME; METABOLISM; PIG; PROCEDURES; ZYGOTE;

ANIMALS; CLONING, ORGANISM; CRISPR-CAS SYSTEMS; GENE EDITING; GENOME; GENOTYPING TECHNIQUES; MICROINJECTIONS; NUCLEAR TRANSFER TECHNIQUES; RNA, GUIDE; SUS SCROFA; ZYGOTE;

EID: 85003875678     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms17122031     Document Type: Article

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