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in Figure SThe adsorption kinetics of PEOXAs at RT and CP is not directly indicative of the remaining swollen thickness recorded for each brush, which was calculated by fitting the initial and the final frequency and dissipation shifts according to an extended viscoelastic model, as described in detail in the Supporting Information. For this reason, the apparent higher adsorbed mass by L-PEOXA-11 at CP with respect to L-PEOXA-6 was not reflected by the formation of a thicker brush. As shown 11 in the Supporting Information, the hydrated mass of L-PEOXA-6 was higher than the corresponding mass of L-PEOXA-11 after rinsing the functionalized sensors by ultra-pure water. It is also noteworthy that under CP conditions the formation of polymer multilayers is likely to take place. For this reason, during both QCM-D and VASE measurements, the samples were rinsed for several hours, until stable and constant values of thickness/mass could be recorded. A comprehensive stability study performed on the different brush films is reported in the Supporting Information
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in Figure SThe adsorption kinetics of PEOXAs at RT and CP is not directly indicative of the remaining swollen thickness recorded for each brush, which was calculated by fitting the initial and the final frequency and dissipation shifts according to an extended viscoelastic model, as described in detail in the Supporting Information. For this reason, the apparent higher adsorbed mass by L-PEOXA-11 at CP with respect to L-PEOXA-6 was not reflected by the formation of a thicker brush. As shown 11 in the Supporting Information, the hydrated mass of L-PEOXA-6 was higher than the corresponding mass of L-PEOXA-11 after rinsing the functionalized sensors by ultra-pure water. It is also noteworthy that under CP conditions the formation of polymer multilayers is likely to take place. For this reason, during both QCM-D and VASE measurements, the samples were rinsed for several hours, until stable and constant values of thickness/mass could be recorded. A comprehensive stability study performed on the different brush films is reported in the Supporting Information.
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2 surface) and the presence of an hydration layer at the brush–medium interface, likely affected the FS profiles measured by C-AFM on the different PEOXA brushes. These contributions resulted in an overestimate of the brush equilibrium swollen thickness by 1 nm as measured by C-AFM for both linear and cyclic PEOXA brushes if compared to the swollen thickness measured by QCM-D.
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