Cloning and expression of streptomycin inactivating enzymes APH(6)-Ia and APH(6)-Id

Cellular and Molecular Biology

Volumn 53, Issue 3, 2007, Pages 74-79

  Collins, A C ^a   Ashenafi, M ^a   Saunders, A A ^a   Byrnes, W Malcolm ^a  

^a HOWARD UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Aminoglycoside phosphotransferase; Expression; Streptomycin

Indexed keywords

ALANINE; AMINO ACID; AMINOGLYCOSIDE ANTIBIOTIC AGENT; APH 6 IA ENZYME; APH 6 ID ENZYME; BACTERIAL ENZYME; ENZYME; HYBRID PROTEIN; NUCLEOTIDE; PHOSPHOTRANSFERASE; SERINE; STREPTOMYCIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; BACTERIAL GENE; CONFERENCE PAPER; ENZYME ACTIVATION; ENZYME INACTIVATION; ENZYME STRUCTURE; ERWINIA AMYLOVORA; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FUNCTION; SITE DIRECTED MUTAGENESIS; TRANSPOSON;

ANIMALIA; ERWINIA AMYLOVORA; ESCHERICHIA COLI; MYCOBACTERIUM TUBERCULOSIS; STREPTOMYCES GRISEUS;

EID: 84995345845     PISSN: 01455680     EISSN: 1165158X     Source Type: Journal    
DOI: 10.1170/T799     Document Type: Article

References (25)

1. Benveniste, R. and Davies, J., Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria. Proc. Natl. Acad. Sci. USA 1973, 70: 2276-2280.
2. Boehr, D.D., Thompson, P.R., and Wright, G.D., Molecular mechanism of aminoglycoside antibiotic kinase APH(3′)-IIIa: Roles of conserved active site residues. J. Biol. Chem. 2001, 276: 23929-23936.
3. Chiou, C.-S. and Jones, A.L., Expression and identification of the strA-strB gene pair from streptomycin-resistant Erwinia amylovora. Gene 1995, 152: 47-51.
4. Davies, J. and Wright, G.D., Bacterial resistance to aminoglycoside antibiotics. Trends Microbiol. 1997, 5: 234-240.
5. Distler, J., Braun, C., Ebert, A. and Piepersberg, W., Gene cluster for streptomycin biosynthesis in Streptomyces griseus: Analysis of a central region including the major resistance gene. Mol. Gen. Genet. 1987a, 208: 204-210.
6. Distler, J., Ebert, A., Mansouri, K. Pissowotzki, K., Stockmann, M. and Piepersberg, W., Gene cluster for streptomycin biosynthesis in Streptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity. Nucleic Acids Res. 1987b, 15: 8041-8056.
7. Fourmy, D., Recht, M.I., Blanchard, S.C. and Puglisi, J.D., Structure of the A site of Escherichia coli 16S ribosomal RNA complexed with an aminoglycoside antibiotic. Science 1996, 274: 1367-371.
8. Hochhut, B., Lotfi, Y., Mazel, D., Faruque, S.M., Woodgate, R. and Waldor, M.K., Molecular analysis of antibiotic resistance gene clusters in Vibrio cholerae O139 and O1 SXT constins. Antimicrob. Agents Chemother. 2001, 45: 2991-3000.
9. Hon, W.-C., McKay, G.A., Thompson, P.R. Sweet, R.M., Yang, D.S.C., Wright, G.D. and Berghuis, A.M., Structure of an enzyme required for aminoglycoside antibiotic resistance reveals homology to eukaryotic protein kinases. Cell 1997, 89: 887-895.
10. Kocabiyik, S. and Perlin, M.H., Site-specific mutations of conserved C-terminal residues in aminoglycoside 3′-phosphotransferase II: Phenotypic and structural analysis of mutant enzymes. Biochem. Biophys. Res. Comm. 1992, 185: 925-931.
11. Lim, C.-K., Smith, M.C.M., Petty, J., Baumberg, S. and Wooton, J.C., Streptomyces griseus streptomycin phosphotransferase: Expression of its gene in Escherichia coli and sequence homology with other antibiotic phosphotransferases and with eukaryotic protein kinases. J. Gen. Microbiol. 1989, 135: 3289-3302.
12. McKay, G.A. and Wright, G.D., Kinetic mechanism of aminoglycoside phosphotransferase type IIIa: Evidence for a Theorell-Chance mechanism. J. Biol. Chem. 1995, 270: 24686-24692.
13. Nurizzo, D., Shewry, S.C., Perlin, M.H., Brown, S.A., Dholakia, J.N., Fuchs, R.L., Deva, T., Baker, E.N. and Smith, C.A., The crystal structure of aminoglycoside-3′-phosphotransferase-IIa, an enzyme responsible for antibiotic resistance. J. Mol. Biol. 2003, 327: 491-506.
14. Piepersberg, W., Streptomycin and related aminoglycosides. In: Genetics and Biochemistry of Antibiotic Production, Vining, LC. and Stuttard, C. (eds.), Butterworth-Heinemann, Halifax, Nova Scotia, 1996, pp. 531-570.
15. Pomp, D. and Medrano, J.F., Organic solvents as facilitators of polymerase chain reaction. Biotechniques, 1991, 10: 58-59.
16. Shaw, K.J., Rather, P.N., Hare, R.S. and Miller, G. H., Molecular genetics of aminioglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol. Rev. 1993, 57: 138-163.
17. Shaw, P.C., Liang, A.C., Kam, K.M. and King, J.M., Presence of strA-strB genes within a streptomycin-resistance operon in a clinical isolate of Shigella flexneri. Pathology 1996, 28: 356-358.
18. Sinkawa, H., Sugiyama, M. and Nimi, O., The nucleotide sequence of the streptomycin 6-phosphotransferase gene from a streptomycin producer. J. Gen. Microbiol. 1987, 133: 1289-1296.
19. Sugiyama, M. Sakamoto, M., Mochizuki, H., Nimi, O. and Nomi, R., Purification and characterization of streptomycin 6-kinase, an enzyme implicated in self-protection of a streptomycin-producing microorganism. J. Gen. Microbiol. 1983, 129: 1683-1687.
20. Sundin, G.W., Examination of base pair variants of the strA-strB streptomycin resistance genes from bacterial pathogens of humans, animals and plants. J. Antimicrob. Chemother. 2000, 46: 847-863.
21. Sundin, G.W. and Bender, C.L., Dissemination of the strA-strB streptomycin-resistance genes among commensal and pathogenic bacteria from humans, animals, and plants. Mol. Ecol. 1996, 5: 13-143.
22. Thompson, P.R., Schwartzenhauer, J., Hughes, D.W., Berghuis, A.M. and Wright, G.D., The COOH terminus of aminoglycoside phosphotransferase (3′)-IIIa is critical for antibiotic recognition and resistance. J. Biol. Chem. 1999, 274: 30697-30706.
23. Thompson, P.R., Boehr, D.D., Berghuis, A.M. and Wright, G.D., Mechanism of aminoglycoside antibiotic kinase APH(3′)-IIIa: Role of the nucleotide positioning loop. Biochemistry 2002, 41: 7001-7007.
24. Wright, G.D., Aminoglycoside-modifying enzymes. Curr. Opin. Microbiol. 1999, 2: 499-503.
25. Wright, G.D. and Thompson, P.R., Aminoglycoside phosphotransferases: Proteins, structure and mechanism. Frontiers Biosci. 1999, 4: 9-21.