Comparing culture and molecular methods for the identification of microorganisms involved in necrotizing soft tissue infections

BMC Infectious Diseases

Volumn 16, Issue 1, 2016, Pages

  Rudkjøbing, Vibeke Børsholt ^a   Thomsen, Trine Rolighed ^a,b   Xu, Yijuan ^a,b   Melton Kreft, Rachael ^c   Ahmed, Azad ^c   Eickhardt, Steffen ^d   Bjarnsholt, Thomas ^d,e   Poulsen, Steen Seier ^d   Nielsen, Per Halkjær ^a   Earl, Joshua P ^f,g,h   Ehrlich, Garth D ^f,g,h,i   Moser, Claus ^e  

^a AALBORG UNIVERSITY   (Denmark)

^b DANISH TECHNOLOGICAL INSTITUTE   (Denmark)

^c ALLEGHENY SINGER RESEARCH INSTITUTE   (United States)

^d UNIVERSITY OF COPENHAGEN   (Denmark)

^e University of Copenhagen   (Denmark)

^f Center for Applied Genomics   (United States)

^g Institute of Molecular Medicine and Infectious Disease   (United States)

^h Departments of Microbiology   (United States)

ⁱ DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

16S rRNA; 454 pyrosequencing; Cloning; Direct Sanger sequencing; FISH; Ibis T5000 biosensor; Microorganisms; Necrotizing soft tissue infections; QPCR

Indexed keywords

RNA 16S;

ACINETOBACTER BAUMANNII; ADULT; AGED; ARTICLE; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; FUNGUS; FUSOBACTERIUM NECROPHORUM; HUMAN; HUMAN TISSUE; MICROBIAL DIVERSITY; MICROBIAL IDENTIFICATION; MOLECULAR CLONING; MYCOPLASMA; NECROTIZING SOFT TISSUE INFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PYROSEQUENCING; QUANTITATIVE ASSAY; RNA SEQUENCE; SOFT TISSUE INFECTION; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS PYOGENES; TURNAROUND TIME; COMPARATIVE STUDY; DEBRIDEMENT; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MIDDLE AGED; NECROSIS; PATHOGENICITY; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS INFECTION;

AGED; BACTERIOLOGICAL TECHNIQUES; DEBRIDEMENT; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MIDDLE AGED; NECROSIS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SOFT TISSUE INFECTIONS; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS PYOGENES;

EID: 84994397490     PISSN: None     EISSN: 14712334     Source Type: Journal    
DOI: 10.1186/s12879-016-1976-2     Document Type: Article

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