Direct demonstration of viable Staphylococcus aureus biofilms in an infected total joint arthroplasty: A case report

Journal of Bone and Joint Surgery

Volumn 90, Issue 8, 2008, Pages 1751-1758

  Stoodley, Paul ^a,b   Nistico, Laura ^a   Johnson, Sandra ^a   Lasko, Leslie Ann ^a   Baratz, Mark ^a   Gahlot, Vikram ^a   Ehrlich, Garth D ^a,b   Kathju, Sandeep ^a,b  

^a ALLEGHENY GENERAL HOSPITAL   (United States)

^b DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CEFALEXIN; CEFAZOLIN; CEFTRIAXONE; CLINDAMYCIN; ERYTHROMYCIN; GATIFLOXACIN; GENTAMICIN; NAFCILLIN; RIFAMPICIN; SULFAMETOXYDIAZINE; VANCOMYCIN;

ADULT; ANAMNESIS; ARTHROPLASTY; ARTICLE; BACTERIUM CULTURE; BIOFILM; CASE REPORT; DEBRIDEMENT; HUMAN; HUMAN TISSUE; HUMERUS FRACTURE; INFECTIOUS ARTHRITIS; MALE; POSTOPERATIVE CARE; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; THORAX RADIOGRAPHY;

EID: 48749118803     PISSN: 00219355     EISSN: None     Source Type: Journal    
DOI: 10.2106/JBJS.G.00838     Document Type: Article

References (21)

1. Costerton JW. Biofilm theory can guide the treatment of device-related orthopaedic infections. Clin Orthop Relat Res. 2005;437:7-11.
2. Ehrlich GD, Hu FZ, Shen K, Stoodley P, Post JC. Bacterial plurality as a general mechanism driving persistence in chronic infections. Clin Orthop Relat Res. 2005;437:20-4.
3. Gristina AG, Costerton JW. Bacterial adherence to biomaterials and tissue. The significance of its role in clinical sepsis. J Bone Joint Surg Am. 1985;67:264-73.
4. Hall-Stoodley L, Hu FZ, Gieseke A, Nistico L, Nguyen D, Hayes J, Forbes M, Greenberg DP, Dice B, Burrows A, Wackym PA, Stoodley P, Post JC, Ehrlich GD, Kerschner JE. Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media. JAMA. 2006;296:202-11.
5. Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet. 2001;358:135-8.
6. Parsek MR, Singh PK. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol. 2003;57:677-701.
7. Stoodley P, Kathju S, Hu FZ, Erdos G, Levenson JE, Mehta N, Dice B, Johnson S, Hall-Stoodley L, Nistico L, Sotereanos N, Sewecke J, Post JC, Ehrlich GD. Molecular and imaging techniques for bacterial biofilms in joint arthroplasty infections. Clin Orthop Relat Res. 2005;437:31-40.
8. Fux CA, Wilson S, Stoodley P. Detachment characteristics and oxacillin resistance of Staphylococcus aureus biofilm emboli in an in vitro catheter infection model. J Bacteriol. 2004;186:4486-91.
9. Mack D, Rohde H, Harris LG, Davies AP, Horstkotte MA, Knobloch JK. Biofilm formation in medical device-related infection. Int J Artif Organs. 2006;29:343-59.
10. Zimmerli W, Trampuz A, Ochsner PE. Prosthetic-joint infections. N Engl J Med. 2004;351:1645-54.
11. Tunney MM, Patrick S, Curran MD, Ramage G, Hanna D, Nixon JR, Gorman SP, Davis RI, Anderson N. Detection of prosthetic hip infection at revision arthroplasty by immunofluorescence microscopy and PCR amplification of the bacterial 16S rRNA gene. J Clin Microbiol. 1999;37:3281-90.
12. Trampuz A, Piper KE, Hanssen AD, Osman DR, Cockerill FR, Steckelberg JM, Patel R. Sonication of explanted prosthetic components in bags for diagnosis of prosthetic joint infection is associated with risk of contamination. J Clin Microbiol. 2006;44:628-31.
13. Neut D, Hendriks JG, van Horn JR, van der Mei HC, Busscher HJ. Pseudomonas aeruginosa biofilm formation and slime excretion on antibiotic-loaded bone cement. Acta Orthop. 2005;76:109-14.
14. Neut D, van Horn JR, van Kooten TG, van der Mei HC, Busscher HJ. Detection of biomaterial-associated infections in orthopaedic joint implants. Clin Orthop Relat Res. 2003;413:261-8.
15. Tunney MM, Patrick S, Gorman SP, Nixon JR, Anderson N, Davis RI, Hanna D, Ramage G. Improved detection of infection in hip replacements. A currently underestimated problem. J Bone Joint Surg Br. 1998;80:568-72.
16. Yugueros J, Temprano A, Sánchez M, Luengo JM, Naharro G. Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene. J Clin Microbiol. 2001;39:3693-5.
17. Rupp CJ, Fux CA, Stoodley P. Viscoelasticity of Staphylococcus aureus biofilms in response to fluid shear allows resistance to detachment and facilitates rolling migration. Appl Environ Microbiol. 2005;71:2175-8.
18. Jones SM, Morgan M, Humphrey TJ, Lappin-Scott H. Effect of vancomycin and rifampicin on methicillin-resistant Staphylococcus aureus biofilms. Lancet. 2001;357:40-1.
19. Saginur R, Stdenis M, Ferris W, Aaron SD, Chan F, Lee C, Ramotar K. Multiple combination bactericidal testing of staphylococcal biofilms from implant-associated infections. Antimicrob Agents Chemother. 2006;50:55-61.
20. Ehrlich GD, Hu FZ, Post JC. Role for biofilms in infectious disease. In: Ghannoum M, O'Toole GA, editors. Microbial biofilms. Washington, DC: ASM Press; 2004. p 332-58.
21. Post JC, Aul JJ, White GJ, Wadowsky RM, Zavoral T, Tabari R, Kerber B, Doyle WJ, Ehrlich GD. PCR-based detection of bacterial DNA after antimicrobial treatment is indicative of persistent, viable bacteria in the chinchilla model of otitis media. Am J Otolaryngol. 1996;17:106-11.