A new method for vitrifying samples for cryoEM

Journal of Structural Biology

Volumn 195, Issue 2, 2016, Pages 190-198

  Razinkov, Ivan ^a   Dandey, Venkata P ^a   Wei, Hui ^a   Zhang, Zhening ^a   Melnekoff, David ^a   Rice, William J ^a   Wigge, Christoph ^a   Potter, Clinton S ^a   Carragher, Bridget ^a  

^a NEW YORK STRUCTURAL BIOLOGY CENTER   (United States)

Author keywords

Automation; CryoTEM; Nanowires; Self blotting grids

Indexed keywords

APOFERRITIN; ICE;

ARTICLE; AUTOMATION; BIOENGINEERING; CAMERA; COMPUTER; CRYOELECTRON MICROSCOPY; GEOMETRY; IMAGE PROCESSING; OPTICAL TWEEZERS; PIEZOELECTRICITY; PRIORITY JOURNAL; RANGE OF MOTION; ROBOTICS; ROOM TEMPERATURE; SCANNING ELECTRON MICROSCOPY; SOFTWARE; TRANSMISSION ELECTRON MICROSCOPY; VITRIFICATION; DEVICES; PROCEDURES; SPECIMEN HANDLING;

CRYOELECTRON MICROSCOPY; ICE; IMAGE PROCESSING, COMPUTER-ASSISTED; SPECIMEN HANDLING; VITRIFICATION;

EID: 84992046823     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2016.06.001     Document Type: Article

References (20)

1. Adrian, M., Dubochet, J., Lepault, J., McDowall, A.W., Cryo-electron microscopy of viruses. Nature 308 (1984), 32–36.
2. Cheng, Y., Single-particle Cryo-EM at crystallographic resolution. Cell 161 (2015), 450–457.
3. Dubochet, J., Adrian, M., Chang, J., Homo, J., Lepault, J., McDowall, A., Schultz, P., Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21 (1988), 129–228.
4. Ermantraut, E., Wohlfart, K., Tichelaar, W., Perforated support foils with pre-defined hole size, shape and arrangement. Ultramicroscopy 74 (1998), 75–81.
5. Fukami, A., Adachi, K., A new method of preparation of a self-perforated micro plastic grid and its application. J. Electron Microsc. (Tokyo) 14 (1965), 112–118.
6. Griffin, B., Joy, D., Probst, C., Suvorova, A., Walck, S., Gauvin, R., Texture and stability and the effect of the sample on thin metal film coatings for high resolution and low voltage SEM. Microsc. Microanal. 13 (2007), 1074–1075.
7. Jain, T., Sheehan, P., Crum, J., Carragher, B., Potter, C.S., Spotiton: a prototype for an integrated inkjet dispense and vitrification system for cryo-TEM. J. Struct. Biol. 179 (2012), 68–75.
8. Kühlbrandt, W., The resolution revolution. Science 343 (2014), 1443–1444.
9. Lander, G.C., Stagg, S.M., Voss, N.R., Cheng, A., Fellmann, D., Pulokas, J., Yoshioka, C., Irving, C., Mulder, A., Lau, P.W., Lyumkis, D., Potter, C.S., Carragher, B., Appion: an integrated, database-driven pipeline to facilitate EM image processing. J. Struct. Biol. 166 (2009), 95–102.
10. Meyerson, J.R., Rao, P., Kumar, J., Chittori, S., Banerjee, S., Pierson, J., Mayer, M.L., Subramaniam, S., Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports. Sci. Rep., 4, 2014, 7084.
11. Quispe, J., Damiano, J., Mick, S.E., Nackashi, D.P., Fellmann, D., Ajero, T.G., Carragher, B., Potter, C.S., An improved holey carbon film for cryo-electron microscopy. Microsc. Microanal. 13 (2007), 365–371.
12. Rigort, A., Bauerlein, F.J., Leis, A., Gruska, M., Hoffmann, C., Laugks, T., Bohm, U., Eibauer, M., Gnaegi, H., Baumeister, W., Plitzko, J.M., Micromachining tools and correlative approaches for cellular cryo-electron tomography. J. Struct. Biol. 172 (2010), 169–179.
13. Rohou, A., Grigorieff, N., CTFFIND4: fast and accurate defocus estimation from electron micrographs. J. Struct. Biol. 192 (2015), 216–221.
14. Roseman, A.M., FindEM – a fast, efficient program for automatic selection of particles from electron micrographs. J. Struct. Biol. 145 (2004), 91–99.
15. Russo, C.J., Passmore, L.A., Ultrastable gold substrates: properties of a support for high-resolution electron cryomicroscopy of biological specimens. J. Struct. Biol. 193 (2016), 33–44.
16. Scheres, S.H., Valle, M., Nunez, R., Sorzano, C.O., Marabini, R., Herman, G.T., Carazo, J.M., Maximum-likelihood multi-reference refinement for electron microscopy images. J. Mol. Biol. 348 (2005), 139–149.
17. Sorzano, C.O., Bilbao-Castro, J.R., Shkolnisky, Y., Alcorlo, M., Melero, R., Caffarena-Fernandez, G., Li, M., Xu, G., Marabini, R., Carazo, J.M., A clustering approach to multireference alignment of single-particle projections in electron microscopy. J. Struct. Biol. 171 (2010), 197–206.
18. Suloway, C., Pulokas, J., Fellmann, D., Cheng, A., Guerra, F., Quispe, J., Stagg, S., Potter, C.S., Carragher, B., Automated molecular microscopy: the new Leginon system. J. Struct. Biol. 151 (2005), 41–60.
19. Taylor, K.A., Glaeser, R.M., Retrospective on the early development of cryoelectron microscopy of macromolecules and a prospective on opportunities for the future. J. Struct. Biol. 163 (2008), 214–223.
20. Zhang, F., Zhang, W.B., Shi, Z., Wang, D., Jin, J., Jiang, L., Nanowire-haired inorganic membranes with superhydrophilicity and underwater ultralow adhesive superoleophobicity for high-efficiency oil/water separation. Adv. Mater. 25 (2013), 4192–4198.