Ultrastable gold substrates: Properties of a support for high-resolution electron cryomicroscopy of biological specimens

Journal of Structural Biology

Volumn 193, Issue 1, 2016, Pages 33-44

  Russo, Christopher J ^a   Passmore, Lori A ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

Cryo EM; Electron cryomicroscopy; Electron tomography; Gold supports; Protein structure; Single particle reconstruction

Indexed keywords

GOLD;

ARTICLE; CARBON FOIL; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; ELECTRON TOMOGRAPHY; FOIL; GEOMETRY; ILLUMINATION; IMAGE ANALYSIS; IMAGE QUALITY; MICROTECHNOLOGY; PRIORITY JOURNAL; STRUCTURE ANALYSIS; YOUNG MODULUS; IMAGE PROCESSING; PROCEDURES;

CRYOELECTRON MICROSCOPY; GOLD; IMAGE PROCESSING, COMPUTER-ASSISTED;

EID: 84954266695     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2015.11.006     Document Type: Article

References (46)

1. Adrian M., Dubochet J., Lepault J., McDowall A.W. Cryo-electron microscopy of viruses. Nature 1984, 308(5954):32-36.
2. Avila-Sakar A.J., Chiu W. Visualization of beta-sheets and side-chain clusters in two-dimensional periodic arrays of streptavidin on phospholipid monolayers by electron crystallography. Biophys. J. 1996, 70(1):57.
3. Bai X.-C., Fernandez I.S., McMullan G., Scheres S.H. Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles. eLife 2013, 2:e00461.
4. Berriman J.A., Rosenthal P.B. Paraxial charge compensator for electron cryomicroscopy. Ultramicroscopy 2012, 116:106-114.
5. Bharat T.A., Russo C.J., Löwe J., Passmore L.A., Scheres S.H. Advances in single-particle electron cryomicroscopy structure determination applied to sub-tomogram averaging. Structure 2015, 23:1743-1753.
6. Blue M., Danielson G. Electrical properties of arc-evaporated carbon films. J. Appl. Phys. 1957, 28(5):583-586.
7. Booy F.P., Pawley J.B. Cryo-crinkling: what happens to carbon films on copper grids at low temperature. Ultramicroscopy 1993, 48(3):273-280.
8. Brilot A.F., Chen J.Z., Cheng A., Pan J., Harrison S.C., Potter C.S., Carragher B., Henderson R., Grigorieff N. Beam-induced motion of vitrified specimen on holey carbon film. J. Struct. Biol. 2012, 177(3):630-637.
9. Bullough P., Henderson R. Use of spot-scan procedure for recording low-dose micrographs of beam-sensitive specimens. Ultramicroscopy 1987, 21(3):223-229.
10. Cheng Y. Single-particle cryo-EM at crystallographic resolution. Cell 2015, 161(3):450-457.
11. Curtis G., Ferrier R. The electric charging of electron-microscope specimens. J. Phys. D: Appl. Phys. 1969, 2(7):1035.
12. Downing K.H., Glaeser R.M. Improvement in high resolution image quality of radiation-sensitive specimens achieved with reduced spot size of the electron beam. Ultramicroscopy 1986, 20(3):269-278.
13. Dubochet J., Adrian M., Chang J.J., Homo J.C., Lepault J., McDowall A.W., Schultz P. Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 1988, 21(2):129-228.
14. Ermantraut E., Wohlfart K., Tichelaar W. Perforated support foils with pre-defined hole size, shape and arrangement. Ultramicroscopy 1998, 74(1):75-81.
15. Franklin R.E. The structure of graphitic carbon. Acta Crystallogr. 1951, 4:253-261.
16. Gjsonnes J. On the structure of evaporated carbon films. Acta Crystallogr. 1960, 13:54.
17. Glaeser R.M. Specimen flatness of thin crystalline arrays: influence of the substrate. Ultramicroscopy 1992, 46(1):33-43.
18. Glaeser R., McMullan G., Faruqi A., Henderson R. Images of paraffin monolayer crystals with perfect contrast: minimization of beam-induced specimen motion. Ultramicroscopy 2011, 111(2):90-100.
19. Handbook of Chemistry and Physics 2015, CRC. 96th ed. W.M. Haynes (Ed.).
20. Henderson R. Overview and future of single particle electron cryomicroscopy. Arch. Biochem. Biophys. 2015, 581:19-24.
21. Henderson, R., 2015b. In practice, thin carbon resistivity varies by several orders of magnitude from one batch to the next under the same nominal conditions. Personal communication.
22. Henderson R., Glaeser R.M. Quantitative analysis of image contrast in electron micrographs of beam-sensitive crystals. Ultramicroscopy 1985, 16:139-150.
23. Kühlbrandt W. The resolution revolution. Science 2014, 343(6178):1443-1444.
24. Landau L., Lifshitz E. Theory of elasticity. Course of Theoretical Physics 1986, vol. 7. Pergamon Press. 3rd ed.
25. Larson D.M., Downing K.H., Glaeser R.M. The surface of evaporated carbon films is an insulating, high-bandgap material. J. Struct. Biol. 2011, 174(2):420-423.
26. Li X., Mooney P., Zheng S., Booth C.R., Braunfeld M.B., Gubbens S., Agard D.A., Cheng Y. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat. Methods 2013, 10(6):584-590.
27. McMullan G., Vinothkumar K., Henderson R. Thon rings from amorphous ice and implications of beam-induced Brownian motion in single particle electron cryo-microscopy. Ultramicroscopy 2015, 158:26-32.
28. Meyerson J.R., Rao P., Kumar J., Chittori S., Banerjee S., Pierson J., Mayer M.L., Subramaniam S. Self-assembled monolayers improve protein distribution on holey carbon cryo-em supports. Sci. Rep. 2014, 4:7084.
29. Mindell J.A., Grigorieff N. Accurate determination of local defocus and specimen tilt in electron microscopy. J. Struct. Biol. 2003, 142(3):334-347.
30. Miyazawa A., Fujiyoshi Y., Stowell M., Unwin N. Nicotinic acetylcholine receptor at 4.6Å resolution: transverse tunnels in the channel wall. J. Mol. Biol. 1999, 288(4):765-786.
31. Mrksich M., Chen C.S., Xia Y., Dike L.E., Ingber D.E., Whitesides G.M. Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold. Proc. Nat. Acad. Sci. 1996, 93(20):10775-10778.
32. Ostuni E., Chapman R.G., Holmlin R.E., Takayama S., Whitesides G.M. A survey of structure-property relationships of surfaces that resist the adsorption of protein. Langmuir 2001, 17(18):5605-5620.
33. Prime K.L., Whitesides G.M. Self-assembled organic monolayers: model systems for studying adsorption of proteins at surfaces. Science 1991, 252(5009):1164-1167.
34. Quispe J., Damiano J., Mick S.E., Nackashi D.P., Fellmann D., Ajero T.G., Carragher B., Potter C.S. An improved holey carbon film for cryo-electron microscopy. Microsc. Microanal. 2007, 13(05):365-371.
35. Reimer L., Kohl H. Transmission Electron Microscopy: Physics of Image Formation 2008, Springer. 5th ed.
36. Rhinow D., Kühlbrandt W. Electron cryo-microscopy of biological specimens on conductive titanium-silicon metal glass films. Ultramicroscopy 2008, 108(7):698-705.
37. Russo C.J., Passmore L.A. Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas. Nat. Methods 2014, 11(6):649-652.
38. Russo C.J., Passmore L.A. Ultrastable gold substrates for electron cryomicroscopy. Science 2014, 346(6215):1377-1380.
39. Sree Harsha K.S. Principles of Physical Vapor Deposition of Thin Films 2006, Elsevier.
40. Sundaram R.S., Steiner M., Chiu H.-Y., Engel M., Bol A.A., Krupke R., Burghard M., Kern K., Avouris P. The graphene-gold interface and its implications for nanoelectronics. Nano Lett. 2011, 11(9):3833-3837.
41. Sze S.M. Physics of Semiconductor Devices 1981, John Wiley & Sons. 2nd ed.
42. Thon F. Zur Defokussierungsabhangigkeit des Phasen-kontrastes bei der elektronenmikroskopischen Abbildung. Z. Naturforsch. A 1966, 21:476-478.
43. Vonck J. Parameters affecting specimen flatness of two-dimensional crystals for electron crystallography. Ultramicroscopy 2000, 85:123-129.
44. Williams R.C., Fisher H.W. Electron microscopy of tobacco mosaic virus under conditions of minimal beam exposure. J. Mol. Biol. 1970, 52:121-123.
45. Gold: Recovery Properties and Applications 1964, D. Van Nostrand Company, Inc. E.M. Wise (Ed.).
46. Yoshioka C., Carragher B., Potter C.S. Cryomesh: a new substrate for cryo-electron microscopy. Microsc. Microanal. 2010, 16(01):43-53.